Code No. | M148-3M | |
Anti-monomeric Kusabira-Green N-terminal fragment mAb | ||
Price | ¥52,800 | |
Size | 100 µL (1 mg/mL) | |
Availability (in Japan) | 1-9
(In Japan at 17:33, Apr 26, 2024 in JST) |
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Clonality | Monoclonal | |
Clone | 1E6 | |
Isotype (Immunized Animal) |
Mouse IgG2b | |
Applications | WB 1 µg/mL IC* (PMID: 22911572) |
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Immunogen (Antigen) |
mKG_N (1-168 a.a.) | |
Storage buffer | 1 mg/mL in PBS/50% glycerol, pH 7.2 | |
Storage temp. | -20°C | |
Conjugate | Unlabeled | |
Manufacturer | MBL | |
Alternative names | mKG_N | |
Background | CoralHue® Fluo-chase Kit can detect protein-protein interactions as fluorescent signals using the protein fragment complementation method. The gene of CoralHue® monomeric Kusabira-Green (mKG), a reporter protein, is divided into two fragments (CoralHue® mKG_N fragment and CoralHue® mKG_C fragment) which are respectively fused to the target protein genes to investigate the interactions. When the expressed target proteins don’t interact, CoralHue® mKG_N fragment and CoralHue® mKG_C fragment cannot approach each other and can not emit fluorescence. However, when target proteins interact, divided CoralHue® mKG fragments spatially approach each other and the local effective concentration increases. As a result, mKG fragments form a steric structure before dividing and the chromophore emits fluorescence. The fluorescent signals can be detected depending on the fused target protein-protein interactions. | |
Related products | M149-3M Anti-monomeric Kusabira-Green C-terminal fragment mAb M168-3M Anti-monomeric Kusabira-Orange 2 mAb |
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Notes | This antibody (clone 1E6) has the epitope on CoralHue® mKG_N fragment and can detect the fusion protein with CoralHue® mKG_N specifically. | |
Product category | Tools:Fluorescent proteins | |
Data | ||
Citations | Immunocytochemistry
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