Antibody detailed product information

Code No.	M133-3
Anti-Atg3 mAb
Price	¥52,800
Size	100 µL (1 mg/mL)
Availability (in Japan)	1-9 （In Japan at 00:05, Apr 25, 2024 in JST）
Clonality	Monoclonal
Clone	3E8
Isotype (Immunized Animal)	Mouse IgG2bκ
Applications	WB　1 µg/mL IP　2.5 µg/300 µL of cell extract from 3x10⁶ cells IC　0.5 μg/mL
Immunogen (Antigen)	Recombinant Human Atg3
Reactivity [Gene ID]	Human[64422], Mouse[67841], Rat[171415], Hamster[100765208]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	autophagy related 3, ATG3, APG3, APG3L, PC3-96, APG3-LIKE
Related products	PM050 Anti-Atg5 pAb M153-3 Anti-Atg5 mAb PM039 Anti-Atg7 (Human) pAb PD042 Anti-Atg9A pAb M154-3 Anti-Atg12 (Human) mAb PM040 Anti-Atg16L pAb M150-3 Anti-Atg16L mAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb PM037 Anti-GABARAP pAb M135-3 Anti-GABARAP mAb PM038 Anti-GATE-16 pAb M134-3 Anti-Atg4B mAb PM072 Anti-VMP1 pAb PM090 Anti-Atg8 (Filamentous fungi) pAb M152-3MS Anti-LC3 mAb for My select sampler set M186-3 Anti-LC3 mAb PD014 Anti-LC3 pAb
Product category	Research area:Autophagy Cellular stress
Data
Citations	Western Blotting Otomo C et al. Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy. Nat Struct Mol Biol. 20, 59-66 (2013)(PMID:24191030) Metlagel Z et al. Structural basis of ATG3 recognition by the autophagic ubiquitin-like protein ATG12. PNAS. 110, 18844-9 (2013)(PMID:24191030) Choi J et al. The parasitophorous vacuole membrane of Toxoplasma gondii is targeted for disruption by ubiquitin-like conjugation systems of autophagy. Immunity. 40, 924-35 (2014)(PMID:24931121) Yamashita SI et al. Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. J Cell Biol. 215, 649-665 (2016)(PMID:27903607) Allavena G et al. Suppressed translation and ULK1 degradation as potential mechanisms of autophagy limitation under prolonged starvation. Autophagy 12, 2085–2097 (2016)(PMID:27629431)

The availability is based on the information in Japan at 00:05, Apr 25, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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