Code No. |
K0055-3 |
Print out |
Anti-FHL2 mAb
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This clone dose not cross-react with FHL1, FHL3, FHL4 and ACT. |
Price |
¥30,000
|
Size |
100 µL (1 mg/mL) |
Availability (in Japan) |
10 or more
(In Japan at 00:05, Mar 29, 2024 in JST)
|
Clonality |
Monoclonal |
Clone |
11-134 |
Isotype
(Immunized Animal) |
Mouse IgG2a |
Applications |
WB 1-5 µg/mL IP 3 µg/200-300 µL of cell extract IC* reported. (PMID: 25855776 / 26320172 / 26973248) IH 1-5 µg/mL (Heat treatment is necessary for paraffin embedded sections.) ChIP* reported. (PMID: 18378678 / 19265191 / 26025865)
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Immunogen
(Antigen) |
Recombinant Human full length FHL2 |
Reactivity [Gene ID] |
Human[2274], Mouse[14200] |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2 |
Storage temp. |
-20°C |
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
four and a half LIM domains 2, DRAL, AAG11, FHL-2, SLIM3, SLIM-3 |
Background |
Proteins containing LIM domains (which are double zinc finger motifs implicated in protein binding) are important regulators of cell growth, cell differentiation, and remodeling of the cell cytoskeleton. Human four-and-a-half LIM domains 2 (FHL2), also known as DRAL/Slim3 is a 32 kDa protein expressed predominantly in human heart and to a lesser extent in skeletal muscle, testis, and prostate epithelium. Since FHL2 is abundant in heart tissue, it may play a role in the regulation of myofibrillogenesis of heart via LIM-domain binding to focal adhesions. FHL2 has also been identified as a coactivator of the androgen receptor where it promotes androgen receptor transcriptional activity. Stimulation of the Rho signaling pathway induces translocation of FHL2 to the nucleus and subsequent activation of FHL2- and androgen receptor-dependent genes. FHL2 also acts as a transcriptional repressor in muscle cells and is involved in modulation of β-catenin-dependent transcription of Wnt-responsive genes. |
Related products |
597 Anti-TRAF6 (Mouse) pAb
|
Product category |
Research area:Epigenetics Cancer |
Data |
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Citations |
Western Blotting - Weinert S et al. M line-deficient titin causes cardiac lethality through impaired maturation of the sarcomere. J Cell Biol. 173, 559-70 (2006)(PMID:16702235)
- Kang DE et al. Presenilins mediate phosphatidylinositol 3-kinase/AKT and ERK activation via select signaling receptors. Selectivity of PS2 in platelet-derived growth factor signaling. J Biol Chem. 280, 31537-47 (2005)(PMID:16014629)
- Sun J et al. FHL2/SLIM3 decreases cardiomyocyte survival by inhibitory interaction with sphingosine kinase-1. Circ Res. 99, 468-76 (2006)(PMID:16888242)
- Wang J et al. Suppression of FHL2 expression induces cell differentiation and inhibits gastric and colon carcinogenesis. Gastroenterology 132, 1066-76 (2007)(PMID:17383428)
- Labalette C et al. The LIM-only protein FHL2 regulates cyclin D1 expression and cell proliferation. J Biol Chem. 283, 15201-8 (2008)(PMID:18378678)
- Labalette C et al. The LIM-only protein FHL2 mediates ras-induced transformation through cyclin D1 and p53 pathways. PLoS One 3, e3761 (2008)(PMID:19018287)
- Ewen EP et al. The Mef2A transcription factor coordinately regulates a costamere gene program in cardiac muscle. J Biol Chem. 286, 29644-53 (2011)(PMID:21724844)
- Hojayev B et al. FHL2 binds calcineurin and represses pathological cardiac growth. Mol Cell Biol. 32, 4025-34 (2012)(PMID:22851699)
- Li SY et al. Four-and-a-Half LIM Domains Protein 2 Is a Coactivator of Wnt Signaling in Diabetic Kidney Disease. J Am Soc Nephrol. 26, 3072-84 (2015)(PMID:25855776)
- Rachmin I et al. FHL2 switches MITF from activator to repressor of Erbin expression during cardiac hypertrophy. Int J Cardiol. 195, 85-94 (2015)(PMID:26025865)
- Yan Q et al. KLF8 promotes tumorigenesis, invasion and metastasis of colorectal cancer cells by transcriptional activation of FHL2. Oncotarget 6, 25402-17 (2015)(PMID:26320172)
- Jin H et al. Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells. Oncogene 35, 5106-18 (2016)(PMID:26973248)
- Wu M et al. FOXK1 interaction with FHL2 promotes proliferation, invasion and metastasis in colorectal cancer. Oncogenesis. 5, e271 (2016)(PMID:27892920)
- Verset L et al. ADAM-17/FHL2 colocalisation suggests interaction and role of these proteins in colorectal cancer. Tumour Biol. 39, 1010428317695024 (2017)(PMID:28349819)
- Zhou SG et al. FHL2 participates in renal interstitial fibrosis by altering the phenotype of renal tubular epithelial cells via regulating the β-βcatenin pathway. Eur Rev Med Pharmacol Sci. 22, 2734-2741 (2018)(PMID:29771425)
ChIP - Labalette C et al. The LIM-only protein FHL2 regulates cyclin D1 expression and cell proliferation. J Biol Chem. 283, 15201-8 (2008)(PMID:18378678)
- Neuman NA et al. The four-and-a-half LIM domain protein 2 regulates vascular smooth muscle phenotype and vascular tone. J Biol Chem. 284, 13202-12 (2009)(PMID:19265191)
- Rachmin I et al. FHL2 switches MITF from activator to repressor of Erbin expression during cardiac hypertrophy. Int J Cardiol. 195, 85-94 (2015)(PMID:26025865)
Immunoprecipitation - Sun J et al. FHL2/SLIM3 decreases cardiomyocyte survival by inhibitory interaction with sphingosine kinase-1. Circ Res. 99, 468-76 (2006)(PMID:16888242)
- Rachmin I et al. FHL2 switches MITF from activator to repressor of Erbin expression during cardiac hypertrophy. Int J Cardiol. 195, 85-94 (2015)(PMID:26025865)
Immunocytochemistry - Li SY et al. Four-and-a-Half LIM Domains Protein 2 Is a Coactivator of Wnt Signaling in Diabetic Kidney Disease. J Am Soc Nephrol. 26, 3072-84 (2015)(PMID:25855776)
- Yan Q et al. KLF8 promotes tumorigenesis, invasion and metastasis of colorectal cancer cells by transcriptional activation of FHL2. Oncotarget 6, 25402-17 (2015)(PMID:26320172)
- Jin H et al. Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells. Oncogene 35, 5106-18 (2016)(PMID:26973248)
- Wu M et al. FOXK1 interaction with FHL2 promotes proliferation, invasion and metastasis in colorectal cancer. Oncogenesis. 5, e271 (2016)(PMID:27892920)
Immunohistochemistry - Wang J et al. Suppression of FHL2 expression induces cell differentiation and inhibits gastric and colon carcinogenesis. Gastroenterology 132, 1066-76 (2007)(PMID:17383428)
- Ewen EP et al. The Mef2A transcription factor coordinately regulates a costamere gene program in cardiac muscle. J Biol Chem. 286, 29644-53 (2011)(PMID:21724844)
- Hojayev B et al. FHL2 binds calcineurin and represses pathological cardiac growth. Mol Cell Biol. 32, 4025-34 (2012)(PMID:22851699)
- Li SY et al. Four-and-a-Half LIM Domains Protein 2 Is a Coactivator of Wnt Signaling in Diabetic Kidney Disease. J Am Soc Nephrol. 26, 3072-84 (2015)(PMID:25855776)
- Yan Q et al. KLF8 promotes tumorigenesis, invasion and metastasis of colorectal cancer cells by transcriptional activation of FHL2. Oncotarget 6, 25402-17 (2015)(PMID:26320172)
- Jin H et al. Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells. Oncogene 35, 5106-18 (2016)(PMID:26973248)
- Wu M et al. FOXK1 interaction with FHL2 promotes proliferation, invasion and metastasis in colorectal cancer. Oncogenesis. 5, e271 (2016)(PMID:27892920)
- Zhou SG et al. FHL2 participates in renal interstitial fibrosis by altering the phenotype of renal tubular epithelial cells via regulating the β-βcatenin pathway. Eur Rev Med Pharmacol Sci. 22, 2734-2741 (2018)(PMID:29771425)
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