Antibody detailed product information
| Code No. | D366-3 | |
| Anti-SaCas9 mAb | ||
| Price | ¥52,800 | |
| Size | 100 µL (1 mg/mL) | |
| Availability (in Japan) | 1-9
（In Japan at 00:05, Apr 24, 2024 in JST） |
|
| Clonality | Monoclonal | |
| Clone | 11E7-9 | |
| Isotype (Immunized Animal) |
Mouse IgG2aκ | |
| Applications | WB 0.5 µg/mL IP 1 µg/sample IC 1 µg/mL |
|
| Reactivity [Gene ID] | Human**, Mouse**, Rat**, Other (Staphylococcus aureus) |
|
| Storage buffer | 1 mg/mL in PBS/50% glycerol, pH 7.2 | |
| Storage temp. | -20°C | |
| Conjugate | Unlabeled | |
| Manufacturer | MBL | |
| Alternative names | Staphylococcus aureus Cas9, Sa Cas9 | |
| Background | The CRISPR/Cas9 system is an innovative tool for genome editing. In 2015, a new Cas9 gene was cloned from Staphylococcus aureus. The SaCas9 (Staphylococcus aureus Cas9) gene is 1 kb shorter than the SpCas9 (Streptococcus pyogenes Cas9) gene, which was originally used for genome editing (Ran FA, et al., 2015, Nature). The protospacer adjacent motif (PAM) sequence of SaCas9, required for DNA cleavage, is longer than that of SpCas9. These molecular characteristics of SaCas9 are expected to enable more efficient genome editing. | |
| Related products | D366-7 Anti-SaCas9 mAb-HRP-DirecT |
|
| Notes | Clone 11E7-9 does not cross-react with SpCas9 (Streptococcus pyogenes Cas9). | |
| Product category | Tools:Others | |
| Data |    |
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| References |
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- The availability is based on the information in Japan at 00:05, Apr 24, 2024 in JST.
- The special price is shown in red color.
- Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
- Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization - For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
- For storage temparature: RT: room temparature
- Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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