Antibody detailed product information

Code No. D346-3
Anti-5-methylcytidine (m5C) mAb
Anti-5-methylcytidine (m5C) mAb (Clone: FMC-9) reacts with 5-methylcytidine (m5C or m5Cyd), 5-methyl-2’-deoxycytidine (m5dC or m5dCyd) and 5-methylcytosine (5mC, 5-mC or m5Cyt).
Price ¥66,000
Size 100 µL (1 mg/mL)
Availability (in Japan) 1-9
（In Japan at 17:00, Apr 26, 2024 in JST）
Clonality Monoclonal
Clone FMC-9
Isotype
(Immunized Animal) 		Mouse IgG2aλ
Applications IP　Can be used.  
IC　5 µg/mL (for cytoplasmic staining), 1 µg/mL (for nuclear staining, HCl-treated)  
IH　10 µg/mL (For nuclear staining, heat treatment or HCl treatment is necessary.)  
ELISA　Inhibition ELISA (Info. from the licenser. Not tested by MBL.)  
DB　Can be used.  
Other(Immuno-Nothern blotting)　Can be used.  （PMID: 26606401）
Immunogen
(Antigen)		 KLH-conjugated 5-methylcytidine (KLH-m5C)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names m5Cyd
Background 5-Methylcytosine (5mC), a modified nucleobase derived from cytosine, is present in both DNA and RNA molecules. 5mC in DNA is an essential epigenetic mark associated with gene silencing and genome stability during embryonic development. 5mC in ribonucleoside, called 5-methylcytidine (m5C), is involved in structural stability, aminoacylation and codon recognition of tRNA. In rRNA, m5C affects translational fidelity and tRNA recognition. m5C-modified nucleobase also have been identified in mRNA and other non-coding RNA but the biological functions are still unclear. m5C is detected not only in tissues and cells but also in urine. It is reported that the levels of m5C change in urine or tissues from patients with various diseases such as bladder, breast and colon cancers.
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RN019M Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
RN016M Anti-7-methylguanosine (m7G)-Cap mAb
RN017M Anti-7-methylguanosine (m7G) mAb
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Product category Research area:RNA-RNP Network Epigenetics
Data





Citations

Dot Blotting

  1. Garvilles RG et al. Dual Functions of the RFTS Domain of Dnmt1 in Replication-Coupled DNA Methylation and in Protection of the Genome from Aberrant Methylation. PLoS One 10, e0137509 (2015)(PMID:26383849)

Immunofluorescence

  1. Totsuka T et al. Production of mouse androgenetic embryos using spindle perturbation. Sci Rep. 10, 6556 (2020)(PMID:32300113)

Other(Immuno-Nothern blotting)

  1. Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
References
  1. Seki Y et al. Extensive and orderly reprogramming of genome-wide chromatin modifications associated with specification and early development of germ cells in mice. Dev Biol. 278, 440-58 (2005)（PMID: 15680362）
  2. Sakai Y et al. Expression of DNA methyltransferase (Dnmt1) in testicular germ cells during development of mouse embryo. Cell Struct Funct. 26, 685-91 (2001)（PMID: 11942627）
  3. Mizugaki M et al. Preparation of a monoclonal antibody specific for 5-methyl-2'-deoxycytidine and its application for the detection of DNA methylation levels in human peripheral blood cells. Biol Pharm Bull. 19, 1537-40 (1996)（PMID: 8996634）
  • The availability is based on the information in Japan at 17:00, Apr 26, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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