Code No. | D336-3 | |
Anti-IDH1 mAb | ||
Anti-IDH1 (Human) mAb (Clone: RcMab-1) reacts with wild type as well as mutated forms of IDH1 proteins such as R132H, R132S, R132L and R132G. | ||
Price | ¥37,400 | |
Size | 100 µL (1 mg/mL) | |
Availability (in Japan) | 10 or more
(In Japan at 00:05, May 13, 2024 in JST) |
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Clonality | Monoclonal | |
Clone | RcMab-1 | |
Isotype (Immunized Animal) |
Rat IgG2aκ | |
Applications | WB 0.5-1 μg/mL IC* reported. (PMID: 23750482) IH* reported. (PMID: 26006098) |
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Reactivity [Gene ID] | ||
Storage buffer | 1 mg/mL in PBS/50% glycerol, pH 7.2 | |
Storage temp. | -20°C | |
Conjugate | Unlabeled | |
Manufacturer | MBL | |
Alternative names | isocitrate dehydrogenase 1 (NADP+), soluble | |
Background | Isocitrate dehydrogenase 1 (IDH1) mutations, which have been identified as early and frequent genetic alterations in astrocytomas, oligodendrogliomas, and oligoastrocytomas, as well as in secondary glioblastomas, might be the initiating event in these glioma subtypes. In contrast, primary glioblastomas, as well as other systemic cancers, rarely contain IDH1 mutations. The IDH1 mutations are remarkably specific to a single codon in the conserved and functionally important Arginine 132 residue (R132) in IDH1. Mutations in the analogous IDH2-R172 codon also occur at a lower rate in these cancers. IDH1/2 mutations were observed subsequently in 22% of acute myeloid leukemias. IDH1 mutations were found to result in the ability of the enzyme to catalyze the reduced NADP-dependent reduction of α-ketoglutarate to R(-)-2-hydroxyglutarate (2-HG). Reduction of α-ketoglutarate by 2-HG or mutant IDH1 results in a lower level of prolyl hydroxylases and promotes the accumulation of hypoxia-inducible factor (HIF) 1α. HIF-1α levels were greater in human gliomas harboring an IDH1 mutation than in tumors without the mutation. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway. | |
Related products | D300-3 Anti-IDH1-R132S (Human) mAb D299-3 Anti-IDH1-R132H (Human) mAb D331-3 Anti-IDH1-R132G (Human) mAb D338-3 Anti-IDH2-R172W (Human) mAb D337-3 Anti-IDH2-R172M (Human) mAb D309-3 Anti-IDH1 mAb D330-3 Anti-IDH2 mAb D328-3 Anti-IDH2-R172K (Human) mAb D332-3 Anti-Mutated IDH1/2 (Human) mAb |
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Product category | Research area:Cancer Neuroscience | |
Data | ||
Citations | Western Blotting
Immunocytochemistry
Immunohistochemistry
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