Antibody detailed product information

Code No. D162-5
Anti-Flavocytochrome b558 (Human) mAb-PE
Price ¥33,000
Size 1 mL (50 tests)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 23, 2024 in JST）
Clonality Monoclonal
Clone 7D5
Isotype
(Immunized Animal) 		Mouse IgG1
Applications FCM　20 µL (Ready for use.)  
Immunogen
(Antigen)		 Human cytochrome b rich fraction
Reactivity [Gene ID]

Human[1535/ 1536], Mouse(-)
Storage buffer PBS/1% BSA/0.09% NaN3
Storage temp. 4°C
Conjugate PE
Manufacturer MBL
Alternative names CYBA, CYBB, p22-PHOX, cytochrome b-245 light chain, p22phox, cytochrome b light chain, p22 phagocyte B-cytochrome
Background The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2-), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2-. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.
Related products D162-3 Anti-Flavocytochrome b558 (Human) mAb
D162-4 Anti-Flavocytochrome b558 (Human) mAb-FITC
Product category Research area:Signal transduction Cellular stress
Data

Citations

Flow Cytometry

  1. Zou J et al. Oxidase-deficient neutrophils from X-linked chronic granulomatous disease iPS cells: functional correction by zinc finger nuclease-mediated safe harbor targeting. Blood 117, 5561-72 (2011)(PMID:21411759)
  2. Kawai C et al. Monoclonal antibody 7D5 recognizes the R147 epitope on the gp91 phox , phagocyte flavocytochrome b 558 large subunit. Microbiol Immunol. 62, 269-280 (2018) (PMID:29573449)
  • The availability is based on the information in Japan at 00:05, Apr 23, 2024 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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