Antibody detailed product information

Code No. BMP001
Anti-HP1α pAb
Price ¥49,500
Size 100 µL (500 µg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 20, 2024 in JST）
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal) 		Rabbit IgG
Applications WB　0.25-1 µg/mL  
IP　1 µg/200 µL of cell extract from 5x106 cells  
IC　10 µg/mL  
IH　10 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
ChIP*　reported.  （PMID: 24561205）
Immunogen
(Antigen)		 synthetic peptide (CHAYPEDAENKEKETAKS) (Human HP1alpha, 175-191 a.a.)
Reactivity [Gene ID]

Human[23468], Mouse[12419], Rat(-)
Storage buffer 500 µg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names chromobox 5, CBX5, HP1A, HEL25
Related products BMP002 Anti-HP1β pAb
BMP003 Anti-HP1γ pAb
Product category Research area:Epigenetics
Data

Citations

Western Blotting

  1. Kashiwagi K et al. DNA methyltransferase 3b preferentially associates with condensed chromatin. Nucleic Acids Res. 39, 874-88 (2011)(PMID:20923784)
  2. Okumura F et al. TRIM8 regulates Nanog via Hsp90β-mediated nuclear translocation of STAT3 in embryonic stem cells. Biochim Biophs Acta. 1813, 1784-92 (2011)(PMID:21689689)
  3. Shiomi Y, Nishitani H. Alternative replication factor C protein, Elg1, maintains chromosome stability by regulating PCNA levels on chromatin. Genes Cells. 18, 946-59 (2013)(PMID:23937667)
  4. Nishibuchi G et al. N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity. Nucleic Acids Res. 42, 12498-511 (2014)(PMID:25332400)

ChIP

  1. Shen WY et al. CTCF-mediated reduction of vigilin binding affects the binding of HP1α to the satellite 2 locus. FEBS Lett. 588, 1549-55 (2014)(PMID:24561205)
  • The availability is based on the information in Japan at 17:00, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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