This site is for customers in Japan. Customers in other regions, please go to Global page.
HOME > Product search results > Code No. RN010MW Anti-PIWIL1 (MIWI) mAb

Code No. RN010MW

Anti-PIWIL1 (MIWI) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 01:30,
Jun 24, 2017 in JST)

Size

100 µg/100 µL

Anti-PIWIL1 (MIWI) pAb (Clone 2D9) shows high specificity for IHC/IF.
Data
  • Immunohistochemistry

  • Immunofluorescence

Clonality Monoclonal Clone 2D9
Isotype (Immunized Animal) Mouse IgG1λ
Applications
IH
0.2 µg/mL (paraffin section)  
IF**
under evaluation  (PMID: 24914035
Reactivity [Gene ID]

Human(-), Mouse[57749], Rat*[363912], Rabbit*[100355343], Marmoset*[100399337](24914035/ 25624265)

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names piwi-like RNA-mediated gene silencing 1
Background Piwi-interacting RNAs (piRNAs) are a distinct class of germ line-specific small non-coding RNAs. piRNAs associate with germ line-specific Argonaute proteins, PIWI proteins, to form piRNA-induced silencing complexes (piRISCs) and function in transposon silencing. In mice, MIWI (PIWIL1), MILI (PIWIL2) and MIWI2 (PIWIL4) are classified as PIWI proteins. Mutations in the Piwi genes cause de-repression of transposons in testes and male sterility. However, each PIWI protein shows different expression pattern through development and associate with distinct populations of piRNAs. MIWI proteins are expressed from pachytene spermatocytes to elongated spermatids and localized in cytoplasmic high electron dense granules known as chromatoid bodies or nuages. MIWI-piRISCs exhibit endonuclease activity, called Slicer. MIWI Slicer mutant male mice show sterility and increases transposon expression, indicating that MIWI-piRISCs regulate transposons by the Slicer activity.
Related products PM043 Anti-PIWIL2 (MILI) (Mouse) pAb
PM044 Anti-PIWIL2 (MILI) (Mouse) pAb
RN028P Anti-EIF2C1 (AGO1) pAb
RN028PW Anti-EIF2C1 (AGO1) pAb
RN003M Anti-EIF2C2 (AGO2) (Human) mAb
RN005M Anti-EIF2C2 (AGO2) mAb
RN029PW Anti-EIF2C2 (AGO2) pAb
RN030PW Anti-DICER1 (Human) pAb
RN057PW Anti-TARBP1 pAb
RN058PW Anti-TARBP2 pAb
RN1005 RIP-Assay Kit for microRNA
M207-3 Anti-MitoPLD (Pld6) mAb
D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb
D356-3 Anti-Jmjd1c (Mouse) mAb
M202-3 Anti-Np95 (Uhrf1) mAb
RN125PW Anti-HENMT1 pAb
D357-3 Anti-Ace (CD143) (Mouse) mAb
D362-3 Anti-Tex101 (Mouse) mAb
D363-3 Anti-Ly6k (Mouse) mAb
Notes *The reactivity of 2D9 to Rat and Rabbit was confirmed by IF with frozen section. (Information from the licenser)
Citations

Immunofluorescence

  1. Hirano H et al. Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate. RNA (2014) In press.(PMID:24914035)
References
  1. Hirano H et al. Small RNA profiling and characterization of piRNA clusters in the adult testes of the common marmoset, a model primate. RNA 20, 1223-37 (2014)(PMID: 24914035
  2. Lin ZY et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol. 400, 43-58 (2015)(PMID: 25624265
Product category
Research area
RNA-RNP Network
Epigenetics
Brands

RiboCluster Profiler™
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 01:30, Jun 24, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.