Anti-OsTIR1 pAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 16, 2024 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Western Blotting
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit IgG
Applications	WB 1:1,000 IC* reported. （PMID: 33177522）
Immunogen (Antigen)	Recombinant full-length Oryza sativa Transport inhibitor response 1 (OsTIR1) protein
Reactivity [Gene ID]	Other (Oryza sativa)
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	Oryza sativa Transport inhibitor response 1
Background	The auxin-inducible degron (AID) system allows the rapid and reversible proteolysis of proteins of interest, which have been invented by Dr. Masato Kanemaki in National Institute of Genetics in Japan. OsTIR1 is a F-box family protein specifically expressed in a rice plant. Since OsTIR1 can function under a culture condition at 37 degree, it is integrated in the AID system to work it in mammalian cells. Briefly, OsTIR1 binds to endogenous Skp1 to form the Skp1-Cullin1-OsTIR1 complex (SCF^TIR1). SCF^TIR1 binds and ubiquitinates the AID tag in the presence of auxin, and promotes proteasome-dependent degradation of the AID-tagged target protein. Human HCT116 cell lines harboring OsTIR1 (CMV-OsTIR1 RCB4662 or Tet-OsTIR1) are available from the RIKEN Cell Bank.
Related products	M214-3 Anti-mini-AID-tag mAb
Citations	Immunocytochemistry Yesbolatova A et al. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 11, 5701 (2020) (PMID:33177522)
References	Natsume T et al. Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Cell Rep. 15, 210-8 (2016)（PMID: 27052166）
Product category	Research area Ubiquitin proteasome Tools Others

The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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