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HOME > Product search results > Code No. M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb

Code No. M218-3

Anti-5-hydroxymethylcytosine (5hmC) mAb

Price

¥60,000

Availability (in Japan)

10 or more

(In Japan at 01:30,
Jun 23, 2017 in JST)

Size

100 µg/100 µL

Anti-5-hydroxymethylcytosine (5hmC) mAb is useful for dot blot and hMeDIP. 5hmC is enriched in brain, liver and stem cells and may function as a regulator of gene activation.
Data
  • Western Blotting

  • Immunoprecipitation

    hMeDIP後のサンプルについて、Spike-in controlの合成DNAに特異的なプライマーを用いてreal-time qPCRを行い、合成DNAに対する%inputを算出した。

  • Immunohistochemistry

Clonality Monoclonal Clone 1G10
Isotype (Immunized Animal) Rabbit IgG
Applications
WB
0.2 μg/mL  
IP
1.0 μg/mL  
IH
Can be used. (paraffin section)  
Immunogen (Antigen) BSA-conjugated 5-hydroxymethylcytidine
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names 5-hmC
Background DNA methylation inheritance is the process of copying pre-existing methylation patterns onto newly replicated DNA strand after DNA replication. Dnmt1 is the maintenance DNA methyltransferase, which methylates hemimethylated CpGs that appear after DNA repairs and replications. The mouse Np95, also known as Uhrf1, is a nuclear protein, which contains an ubiquitin-like domain, a tandem Tudor domain, a PHD (Plant Homeodomain) finger, an SRA (SET-and RING-associated) domain, and a RING (Really Interesting New Gene) finger domain. Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.
Related products PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb
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PM035 Normal Rabbit IgG
PM085 Anti-ZBTB24 pAb
PM086 Anti-ZBTB24 (Human) pAb
M227-3 Anti-Digoxigenin (DIG) mAb
M229-3 Anti-MECP2 mAb
M228-3 Anti-FITC mAb
RN019M Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
RN131P Anti-N6-methyladenosine (m6A) pAb
RN016M Anti-7-methylguanosine (m7G)-Cap mAb
RN017M Anti-7-methylguanosine (m7G) mAb
PM098 Anti-Inosine pAb
Notes Available in China and Japan only.
Product category
Research area
Epigenetics
Brands
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  • The availability is based on the information in Japan at 01:30, Jun 23, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.