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HOME > Product search results > Code No. M207-3 Anti-MitoPLD (Pld6) mAb

Code No. M207-3

Anti-MitoPLD (Pld6) mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 01:30,
Jun 29, 2017 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Immunohistochemistry

Clonality Monoclonal Clone 26C46-6
Isotype (Immunized Animal) Mouse IgG2bκ
Applications
WB
2-5 µg/mL  
IP
3 µg/mL  
IH
10 µg/mL  
Immunogen (Antigen) KLH conjugated synthetic peptide, EFDPTKYSFFPQKHRGH (corresponding to amino acidresidues 205-221 of mouse MitoPLD (Pld6)
Reactivity [Gene ID]

Mouse[194908]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names mZuc (Zucchini), Zuc, Gm10, phospholipase D family, member 6
Background MitoPLD (Pld6), the ortholog of drosophila Zucchini (Zuc), is a member of the phospholipase D superfamily. MitoPLD is strongly expressed in gonads especially in testis, and is located on the outer mitochondrial membrane. It is reported that MitoPLD hydrolyzes cardiolipin to generate phosphatidic acid (PA) at mitochondrial surface, and promotes mitochondrial fusion. PA, generated by MitoPLD, may work as a signaling molecule to regulate the localization and formation of mitochondria and intermitochondrial cement (nuage). It is also reported that the drosophila MitoPLD ortholog, Zuc, is an endoribonuclease essential for primary piRNA biogenesis by processing long non-coding RNA precursors. MitoPLD-/- mice show meiotic arrest during spermatogenesis, demethylation and derepression of retrotransposons, and defects in primary piRNA biogenesis, indicating that the role of MitoPLD/Zuc in the piRNA pathway is highly conserved among species.
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Citations

Western Blotting

  1. Kabayama Y et al. Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes. Nucleic Acids Res. 45, 5387-5398 (2017)(PMID:28115634)
References
  1. Nishimasu H et al. Structure and function of Zucchini endoribonuclease in piRNA biogenesis. Nature. 491, 284-7 (2012)(PMID: 23064230
  2. Gao Q, Frohman MA. Roles for the lipid-signaling enzyme MitoPLD in mitochondrial dynamics, piRNA biogenesis, and spermatogenesis. BMB Rep. 45, 7-13 (2012)(PMID: 22281006
  3. Huang H et al. piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling. Dev Cell. 20, 376-87 (2011)(PMID: 21397848
  4. Watanabe T et al. MITOPLD is a mitochondrial protein essential for nage formation and piRNA biogenesis in the mouse germline. Dev Cell. 20, 364-75 (2011)(PMID: 21397847
  5. Choi SY et al. A common lipid links Mfn-mediated mitochondrial fusion and SNARE-regulated exocytosis. Nat Cell Biol. 8, 1255-62 (2006)(PMID: 17028579
Product category
Research area
Stem cell research
RNA-RNP Network
Epigenetics
Brands
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  • The availability is based on the information in Japan at 01:30, Jun 29, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.