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HOME > Product search results > Code No. M177-3 Anti-β-Actin mAb

Code No.　M177-3

Anti-β-Actin mAb

Price

¥27,500

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunoprecipitation
Clonality Monoclonal Clone 6D1
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
WB
1 µg/mL  
IP
2 µg/200 µL of cell extract from 2 x 106 cells  
Immunogen (Antigen) N-terminal β-Actin synthetic peptide
Reactivity [Gene ID]

Human, Mouse, Rat, Hamster, Chicken, Porcine*(PMID: 32768229)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Actin is a 42 kDa protein found in eukaryotic cells. Actin is also one of the most highly conserved proteins. Actin participates in many important cellular activities including muscle contraction, cell movement, cell division and the formation of the cytoskeleton. The beta actin exists in most cell types as components of the cytoskeleton.
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Citations

Western Blotting

  1. Watanabe-Nakayama T et al. Requirement of LIM domains for the transient accumulation of paxillin at damaged stress fibres. Biol Open. 2, 667-74 (2013)(PMID:23862014)
  2. Xing M et al. Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway. Nat Commun. 6, 6233 (2015)(PMID:25670504)
  3. Fukuda T et al. Autophagy inhibition augments resveratrol-induced apoptosis in Ishikawa endometrial cancer cells. Oncol Lett. 12, 2560-2566 (2016)(PMID:27698828)
  4. Sun H et al. Resistance of glioma cells to nutrient-deprived microenvironment can be enhanced by CD133-mediated autophagy. Oncotarget 7, 76238-76249 (2016)(PMID:27780926)
  5. Song KH et al. REP1 inhibits FOXO3-mediated apoptosis to promote cancer cell survival. Cell Death Dis. 8, e2536 (2017)(PMID:28055019)
  6. Hori I et al. Defects in autophagosome-lysosome fusion underlie Vici syndrome, a neurodevelopmental disorder with multisystem involvement. Sci Rep. 7, 3552 (2017)(PMID:28615637)
  7. Yoshioka H et al. Sasa veitchii extracts suppress acetaminophen-induced hepatotoxicity in mice. Environ Health Prev Med. 22, 54 (2017)
  8. Xu J et al. Nuclear carbonic anhydrase 6B associates with PRMT5 to epigenetically promote IL-12 expression in innate response. PNAS 114, 8620-8625 (2017)(PMID:28739930)
  9. Tsurudome M et al. The Hemagglutinin-Neuraminidase (HN) Head Domain and the Fusion (F) Protein Stalk Domain of the Parainfluenza Viruses Affect the Specificity of the HN-F Interaction. Front Microbiol. 9, 391 (2018)(PMID:29593671)
  10. Ohkuro M et al. Calreticulin and integrin alpha dissociation induces anti-inflammatory programming in animal models of inflammatory bowel disease. Nat Commun. 9, 1982 (2018)(PMID:29773794)
  11. Nakano T et al. Hepatitis C virus core protein triggers abnormal porphyrin metabolism in human hepatocellular carcinoma cells. PLoS One. 13, e019834 (2018)(PMID:29856826)
  12. Ji M et al. Hepatitis B core VLP-based mis-disordered tau vaccine elicits strong immune response and alleviates cognitive deficits and neuropathology progression in Tau.P301S mouse model of Alzheimer's disease and frontotemporal dementia. Alzheimers Res Ther. 10, 55 (2018)(PMID:29914543)
  13. Fujimoto M et al. Transcriptional repression of human epidermal growth factor receptor 2 by ClC-3 Cl- /H+ transporter inhibition in human breast cancer cells. Cancer Sci. 109, 2781-2791 (2018)(PMID:29949674)
  14. Yang L et al. Reconstituting Arabidopsis CRY2 Signaling Pathway in Mammalian Cells Reveals Regulation of Transcription by Direct Binding of CRY2 to DNA. Cell Rep. 24, 585-593.e4 (2018)(PMID:30021157)
  15. Tong L et al. RDM1 plays an oncogenic role in human lung adenocarcinoma cells. Sci Rep. 8, 11525 (2018)(PMID:30069034)
  16. Yasuoka Y et al. Fludrocortisone stimulates erythropoietin production in the intercalated cells of the collecting ducts. Biochem Biophys Res Commun. 503, 3121-3127 (2018)(PMID:30146260)
  17. Gao S et al. An OB-fold complex controls the repair pathways for DNA double-strand breaks. Nat Commun. 9, 3925 (2018)(PMID:30254264)
  18. Park OJ et al. Streptococcus gordonii induces bone resorption by increasing osteoclast differentiation and reducing osteoblast differentiation. Microb Pathog. 126, 218-223 (2018)(PMID:30414445)
  19. Azami T et al. Klf5 suppresses ERK signaling in mouse pluripotent stem cells. PLoS One 13, e0207321 (2018)(PMID:30452437)
  20. Shu T et al. Ebola virus VP35 has novel NTPase and helicase-like activities. Nucleic Acids Res. 47, 5837-5851 (2019)(PMID:31066445)
  21. Song KH et al. HSP90A Inhibition Promotes Anti-Tumor Immunity by Reversing Multi-Modal Resistance and Stem-Like Property of Immune-Refractory Tumors. Nat Commun. 11, 562 (2020)(PMID:31992715)
  22. Saotome H et al. Generation of a Quantitative Luciferase Reporter for Sox9 SUMOylation. Int J Mol Sci. 21, 1274 (2020)(PMID:32070068)
  23. Yasuoka Y et al. Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation. Physiol Rep. 8, e14485 (2020)(PMID:32592328)
  24. Naoto Matsumoto N et al. PP1C and PP2A are p70S6K Phosphatases Whose Inhibition Ameliorates HLD12-Associated Inhibition of Oligodendroglial Cell Morphological Differentiation. Biomedicines. 8, 89 (2020)(PMID:32316234)
  25. Liu S et al. Porcine deltacoronavirus (PDCoV) infection antagonizes interferon-λ1 production. Vet Microbiol. 247, 108785 (2020)(PMID:32768229)
  26. Ichimaru Y et al. Sasa veitchii extract induces anticancer effects via inhibition of cyclin D1 expression in MCF-7 cells. Nagoya J Med Sci. 82, 509-518 (2020)(PMID:33132435)
  27. Gao X et al. The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells. Nat Commun. 11, 3904 (2020)(PMID:32764536)
  28. Yamamuro T et al. Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy. Nat Commun. 11, 4150 (2020) (PMID:32811819)
  29. Chida J et al. Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice. PLoS Pathog. 16, e1008823 (2020) (PMID:32845931)
  30. Sakane H et al. Involvement of lysosomal integral membrane protein-2 in the activation of autophagy. Biochem Biophys Res Commun. 533, 976-982 (2020) (PMID:33010890)
  31. Miyata S et al. Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice. Mol Brain. 14, 5 (2020) (PMID:33413507)
Product category
Tools
Control antibodies
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  • The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.