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HOME > Product search results > Code No. D345-3 Anti-1-methyladenosine (m1A) mAb

Code No.　D345-3

Anti-1-methyladenosine (m1A) mAb

Price

¥66,000

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 18, 2024 in JST）

Size

100 µL (1 mg/mL)

Data

  • Immunoprecipitation

  • Immunocytochemistry

  • Immunohistochemistry
Clonality Monoclonal Clone AMA-2
Isotype (Immunized Animal) Mouse IgG2bκ
Applications
IP
15 µg/sample  
IC
1 µg/mL  
IH
0.1 µg/mL  
ELISA**
Inhibition ELISA (Info. from the licenser. Not tested by MBL.)  
DB*
reported.  （PMID: 26863196 / 26863410）
Other(Immuno-Nothern blotting)
  （PMID: 26606401）
Immunogen (Antigen) KLH-conjugated 1-methyladenosine (KLH-m1A)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names m1Ado
Background In eukaryote, newly synthesized RNA is chemically modified at specific nucleotide position after transcription. N1-methyladenosine (m1A) is one of major modified nucleosides present in most eukaryotic tRNAs and also found in yeast rRNAs. m1A affects structural stability of tRNA and its binding to polysome in translation. Urinary excretion of modified nucleosides including m1A is known to be increased in the patients with various cancers and AIDS. It is reported that the amount of urinary m1A correlates with m1A level in tissue from same patients with esophageal carcinoma. Moreover, elevated serum levels of m1A are also detected under stress conditions.
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Citations

Dot Blotting

  1. Dominissini D et al. The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA. Nature 530, 441-6 (2016)(PMID:26863196)
  2. Li X et al. Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome. Nat Chem Biol. 12, 311-6 (2016)(PMID:26863410)
  3. Liu F et al. ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell 167, 816-828.e16. (2016)(PMID:27745969)
  4. Ueda Y et al. AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells. Sci Rep. 7, 42271 (2017)(PMID:28205560)

Northern Blotting

  1. Elkordy A et al. tiRNAs as a novel biomarker for cell damage assessment in in vitro ischemia-reperfusion model in rat neuronal PC12 cells. Brain Res. 1714, 8-17 (2019)(PMID:30779912)

Immunoprecipitation

  1. Dominissini D et al. The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA. Nature 530, 441-6 (2016)(PMID:26863196)
  2. Li X et al. Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome. Nat Chem Biol. 12, 311-6 (2016)(PMID:26863410)
  3. Waku et al. NML-mediated rRNA base methylation links ribosomal subunit formation to cell proliferation in a p53-dependent manner. J. Cell Sci. 129, 2382-93 (2016)(PMID:27149924)
  4. Safra M et al. The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution. Nature 551, 251-255 (2017)(PMID:29072297)
  5. Li X et al. Base-Resolution Mapping Reveals Distinct m1A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts. Mol Cell  68, 993-1005 (2017)(PMID:29107537)

Immunocytochemistry

  1. Ueda Y et al. AlkB homolog 3-mediated tRNA demethylation promotes protein synthesis in cancer cells. Sci Rep. 7, 42271 (2017)(PMID:28205560)

Other(Immuno-Nothern blotting)

  1. Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
  2. Alaa E et al. Stress-induced tRNA cleavage and tiRNA generation in rat neuronal PC12 cells. J Neurochem. (2018) In press.(PMID:29431851)
  3. Mishima E and Abe T. Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified Nucleosides. Methods Mol Biol. 1870, 179-187 (2019) (PMID:30539555)
  4. Sato K et al. Stress Induced tRNA Halves (tiRNAs) as Biomarkers for Stroke and Stroke Therapy; Pre-clinical Study. Neuroscience. 434, 44-54 (2020)(PMID:32200075)
References
  1. Itoh K et al. Preparation of a monoclonal antibody specific for 1-methyladenosine and its application for the detection of elevated levels of 1-methyladenosine in urines from cancer patients. Jpn J Cancer Res. 79, 1130-8 (1988)（PMID: 3143701）
  2. Itoh K et al. Diagnostic use of anti-modified nucleoside monoclonal antibody. Tohoku J Exp Med. 168, 329-31 (1992)1306318（PMID: 1306318）
  3. Masuda M et al. An immunohistochemical analysis for cancer of the esophagus using monoclonal antibodies specific for modified nucleosides.Cancer 72, 3571-8 (1993)（PMID: 8252470）
  4. Mishima E et al. Conformational Change in Transfer RNA Is an Early Indicator of Acute Cellular Damage. J Am Soc Nephrol. 25, 2316-26 (2014)（PMID: 24833129）
  5. Chi KR. The RNA code comes into focus. Nature 542, 503-506 (2017)（PMID: 28230128）
Product category
Research area
RNA-RNP Network
Epigenetics
Cancer
Cellular stress
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  • The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.