Anti-Sap155 mAb

Price

¥52,800

Availability (in Japan)

1-9

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting
Clonality	Monoclonal	Clone	1A5
Isotype (Immunized Animal)	Mouse IgG2b
Applications	WB 1 µg/mL
Immunogen (Antigen)	Recombinant Mouse Sap155
Reactivity [Gene ID]	Human[23451], Mouse[81898](PMID: 20153721), Hamster
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	SF3B1, MDS, PRP10, Hsh155, PRPF10, SAP155, SF3b155
Background	SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the major spliceosome. SF3 contains the Spliceosome-Associated Proteins, SAP 49, 130, 145, and 155. SAP155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) spliceosome. SAP155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two-hybrid system. SAP155 contains numerous Cdk consensus phosphorylation sites in its N terminus and is phosphorylated prior to catalytic step II of the splicing pathway. SAP155 serves as a substrate for cyclin E-cdk2 in vitro, suggesting that pre-mRNA splicing may be linked to the cell cycle machinery in mammalian cells.
Related products	D221-3 Anti-Sap155 mAb D139-3 Anti-Ring1B mAb RN085PW Anti-U2AF1 pAb
Citations	Western Blotting Kotake et al. Splicing factor SF3b as a target of the antitumor natural product pladienolide. Nat Chem Biol. 3, 570-5 (2007)(PMID:17643112) Eto K et al. Phosphorylated SAP155, the spliceosomal component, is localized to chromatin in postnatal mouse testes. Biochem Biophys Res Commun. 393, 577-81 (2010)(PMID:20153721) Eto K et al. The kinase DYRKIA regulates pre-mRNA splicing in spermatogonia and proliferation of spermatogonia and Sertoli cells by phosphorylating a spliceosomal component, SAP155, in postnatal murine testes. Mol Cell Biochem. 355, 217-22 (2011)(PMID:21553260) Atlasi Y et al. Wnt signaling regulates the lineage differentiation potential of mouse embryonic stem cells through Tcf3 down-regulation. PLoS Genet. 9, e1003424 (2013)(PMID:23658527) Kralovicova J et al. Exon-centric regulation of ATM expression is population-dependent and amenable to antisense modification by pseudoexon targeting. Sci Rep. 6, 18741 (2016)(PMID:26732650) De Maio A et al. RBM17 Interacts with U2SURP and CHERP to Regulate Expression and Splicing of RNA-Processing Proteins. Cell Rep. 25, 726-736.e7 (2018)(PMID:30332651) Immunoprecipitation Eto K et al. Phosphorylated SAP155, the spliceosomal component, is localized to chromatin in postnatal mouse testes. Biochem Biophys Res Commun. 393, 577-81 (2010)(PMID:20153721)
Product category	Research area RNA-RNP Network Epigenetics Cancer

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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