Anti-IL-18 (Mouse) mAb (Functional Grade)

Price

¥79,200

Availability (in Japan)

1-9

（In Japan at 00:05,
Apr 25, 2024 in JST）

Size

100 µL (1 mg/mL)

Data Sheet

SDS

Data	Immunoprecipitation Neutralization Induction of IFN-γ by mouse IL-18 receptor transfected KG-1 cell (KG-1 cell: Human myelomonocyte: ATCC CCL246) in response to the 30 ng/mL recombinant mouse IL-18 was neutralized by this antibody. This table shows neutralization activity of lot 001.
Clonality	Monoclonal	Clone	93-10C
Isotype (Immunized Animal)	Rat IgG1
Applications	IP 2 μg/0.2 μg of recombinant mouse IL-18 NT 0.5-5 µg/mL
Immunogen (Antigen)	Mouse IL-18 (recombinant)
Reactivity [Gene ID]	Mouse[16173]
Storage buffer	1 mg/mL in PBS. Azide free, 0.22 μm sterile-filtered
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	interleukin 18 (interferon-gamma-inducing factor), IGIF, IL1F4, IL-1g, IL-18
Background	Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
Related products	D048-3 Anti-IL-18 (Mouse) mAb D048-6 Anti-IL-18 (Mouse) mAb-Biotin D046-3 Anti-IL-18 (Mouse) mAb D047-3 Anti-IL-18 (Mouse) mAb 7625 Mouse IL-18 ELISA Kit B002-5 Recombinant Mouse IL-18 B004-5 Recombinant Mouse IL-18 (without BSA) M080-3M2 Rat IgG1 (isotype control) (Functional Grade) D045-3 Anti-IL-18 (Human) mAb
References	Okamura H et al. Cloning of a new cytokine that induces IFN-gamma production by T cells. Nature 378, 88-91 (1995)（PMID: 7477296） Ushio S et al. Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein. J Immunol. 156, 4274-9 (1996)（PMID: 8666798） Micallef M et al. Interferon-gamma-inducing factor enhances T helper 1 cytokine production by stimulated human T cells: synergism with interleukin-12 for interferon-gamma production. Eur J Immunol. 26, 1647-51 (1996)（PMID: 8766574） Dao T et al. Interferon-gamma-inducing factor, a novel cytokine, enhances Fas ligand-mediated cytotoxicity of murine T helper 1 cells. Cell Immunol. 173, 230-5 (1996)（PMID: 8912881） Rogers TB et al. Mesenchymal stem cells stimulate protective genetic reprogramming of injured cardiac ventricular myocytes. J Mol Cell Cardiol. 50, 346-56 (2011)（PMID: 20837021） Zanoni I et al. IL-15 cis presentation is required for optimal NK cell activation in lipopolysaccharide-mediated inflammatory conditions. Cell Rep. 26, 1235-49 (2013)（PMID: 24055061） Hirano Y et al. IL-18 is not therapeutic for neovascular age-related macular degeneration. Nat Med. 20, 1372-5 (2014)（PMID: 25473914） Abdel Fattah E et al. Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency. J Immunol. 194, 5407-16 (2015)（PMID: 25888640）
Product category	Research area Immunology Cytokine & Growth Factors

The availability is based on the information in Japan at 00:05, Apr 25, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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