Anti-IL-18 (Human) mAb (Functional Grade)

Price

¥79,200

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 16, 2024 in JST）

Size

100 µL (1 mg/mL)

Data Sheet

SDS

Data	Immunoprecipitation Neutralization Induction of IFN-γ by human IL-18 receptor expressed on KG-1 cell (KG-1 cell: Human myelomonocyte: ATCC CCL246) in response to the 40 ng/mL recombinant human IL-18 was neutralized by this antibody. This table shows neutralization activity of l
Clonality	Monoclonal	Clone	125-2H
Isotype (Immunized Animal)	Mouse IgG1 κ
Applications	IP 2 μg/0.2 μg recombinant human IL-18 NT 0.1-1 µg/mL
Immunogen (Antigen)	Human IL-18 (recombinant)
Reactivity [Gene ID]	Human[3606], Mouse(-)
Storage buffer	1 mg/mL in 0.1M NaPB (pH 6.0)/0.15 M NaCl. Azide free, 0.22 μm sterile-filtered
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	interleukin 18 (interferon-gamma-inducing factor), IGIF, IL1F4, IL-1g, IL-18
Background	Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-γ in T cells, enhancement of Fas ligand expression by Th1 cells.
Related products	D043-3 Anti-IL-18 (Human) mAb D044-3 Anti-IL-18 (Human) mAb D045-3 Anti-IL-18 (Human) mAb D045-6 Anti-IL-18 (Human) mAb-Biotin PM014 Anti-IL-18 (Human) pAb 7620 Human IL-18 ELISA Kit B001-5 Recombinant Human IL-18 B003-5 Recombinant Human IL-18 (without BSA) M075-3M2 Mouse IgG1 (isotype control) (Functional Grade)
References	Okamura H et al. Cloning of a new cytokine that induces IFN-gamma production by T cells. Nature 378, 88-91 (1995)（PMID: 7477296） Ushio S et al. Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein. J Immunol. 156, 4274-9 (1996)（PMID: 8666798） Micallef M et al. Interferon-gamma-inducing factor enhances T helper 1 cytokine production by stimulated human T cells: synergism with interleukin-12 for interferon-gamma production. Eur J Immunol. 26, 1647-51 (1996)（PMID: 8766574） Dao T et al. Interferon-gamma-inducing factor, a novel cytokine, enhances Fas ligand-mediated cytotoxicity of murine T helper 1 cells. Cell Immunol. 173, 230-5 (1996)（PMID: 8912881） Sugawara S et al. Neutrophil proteinase 3-mediated induction of bioactive IL-18 secretion by human oral epithelial cells. J Immunol. 167, 6568-75 (2001)（PMID: 11714826） Wu C et al. IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction. J Immunol. 170, 5571-7 (2003)（PMID: 12759435） Tu A et al. TLR-dependent cross talk between human Kupffer cells and NK cells. J Exp Med. 205, 233-44 (2008)（PMID: 18195076） Nussbaumer O et al. DC-like cell-dependent activation of human natural killer cells by the bisphosphonate zoledronic acid is regulated by γδ T lymphocytes. Blood 118, 2743-51 (2011)（PMID: 21673342）
Product category	Research area Immunology Cytokine & Growth Factors

The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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