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HOME > Product search results > Code No. 562-5 Anti-Myc-tag pAb

Code No. 562-5

Anti-Myc-tag pAb

Price

¥88,000

Availability (in Japan)

10 or more

(In Japan at 17:00,
Apr 20, 2024 in JST)

Size

500 µL

Data
  • Immunocytochemistry

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit IgG
Applications
WB
1:1,000  
IP
2 µL/sample  
IC
1:250  
IH*
reported.  (PMID: 18552836 / 19168675
ELISA*
reported.  (PMID: 23526492
ChIP*
reported.  (PMID: 21573214
FCM*
reported.  (PMID: 29966723
Immunogen (Antigen) synthetic peptide EQKLISEEDL (Myc-tag)-KLH
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Background Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
Related products 562 Anti-Myc-tag pAb
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M047-10 Anti-Myc-tag mAb-Magnetic Agarose
M047-A48 Anti-Myc-tag mAb-Alexa Fluor™ 488
M047-A59 Anti-Myc-tag mAb-Alexa Fluor™ 594
M047-A64 Anti-Myc-tag mAb-Alexa Fluor™ 647
M192-3 Anti-Myc-tag mAb
M192-7 Anti-Myc-tag mAb-HRP-DirecT
M047-11 Anti-Myc-tag mAb-Magnetic Beads
M192-6 Anti-Myc-tag mAb-Biotin
Citations

Western Blotting

  1. Matsushita T et al. Functional SNP of ARHGEF10 confers risk of atherothrombotic stroke. Hum Mol Genet. 19, 1137-46 (2010)(PMID:20042462)
  2. Xue JF et al. DEDD negatively regulates transforming growth factor-beta1 signaling by interacting with Smad3. FEBS Lett. 584, 3028-34 (2010)(PMID:20553715)
  3. Bai N et al. Dock3 interaction with a glutamate-receptor NR2D subunit protects neurons from excitotoxicity. Mol Brain 6, 22 (2013)(PMID:23641686)
  4. Sato M et al. Development and validation of scFv-conjugated affinity silk protein for specific detection of carcinoembryonic antigen. Sci Rep. 7, 16077 (2017)(PMID:29167497)
  5. Komiya M et al. A genetic screen to discover SUMOylated proteins in living mammalian cells. Sci Rep. 7, 17443 (2017)(PMID:29234079)
  6. Baba K et al. Gradient-reading and mechano-effector machinery for netrin-1-induced axon guidance. Elife 7, e34593 (2018)(PMID:30082022)
  7. Wang H et al. A potato STRUBBELIG-RECEPTOR FAMILY member, StLRPK1, associates with StSERK3A/BAK1 and activates immunity. J Exp Bot. 69, 5573-5586 (2018)(PMID:30137408)
  8. Rasila T et al. Astroprincin (FAM171A1, C10orf38): A Regulator of Human Cell Shape and Invasive Growth. Am J Pathol. 189, 177-189 (2019)(PMID:30312582)
  9. Sun P et al. A Mosquito Salivary Protein Promotes Flavivirus Transmission by Activation of Autophagy. Nat Commun. 11, 260 (2020)(PMID:31937766)
  10. Nishida T, Yamada Y. RNF4-mediated SUMO-targeted ubiquitination relieves PARIS/ZNF746-mediated transcriptional repression. Biochem Biophys Res Commun. 526, 110-116 (2020)(PMID:32197837)
  11. Hitomi S et al. Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells. In Vivo. 34, 2437-2441 (2020) (PMID: 32871770)

ChIP

  1. Isogai E et al. Oncogenic LMO3 collaborates with HEN2 to enhance neuroblastoma cell growth through transactivation of Mash1. PLoS One 6, e19297 (2011)(PMID:21573214)

Immunoprecipitation

  1. Xue JF et al. DEDD negatively regulates transforming growth factor-beta1 signaling by interacting with Smad3. FEBS Lett. 584, 3028-34 (2010)(PMID:20553715)

Flow Cytometry

  1. Gomi F et al. Up-regulation of NSP3 by Oligomeric Aβ Accelerates Neuronal Death Through Cas-independent Rap1A Activation. Neuroscience 386, 182-193 (2018)(PMID:29966723)

Immunocytochemistry

  1. Fukunaka A et al. Tissue nonspecific alkaline phosphatase is activated via a two-step mechanism by zinc transport complexes in the early secretory pathway. J Biol Chem. 286, 16363-73 (2011)(PMID:21402707)
  2. Nakamura S et al. Gene therapy for a mouse model of glucose transporter-1 deficiency syndrome. Mol Genet Metab Rep. 10, 67-74 (2017)(PMID:28119822)

Immunohistochemistry

  1. Nakaya Y et al. RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation. Nat Cell Biol. 10, 765-75 (2008)(PMID:18552836)
  2. Shin M et al. Notch mediates Wnt and BMP signals in the early separation of smooth muscle progenitors and blood/endothelial common progenitors. Development 136, 595-603 (2009)(PMID:19168675)
  3. Hiramatsu N et al. ER membrane protein complex is required for the insertions of late-synthesized transmembrane helices of Rh1 in Drosophila photoreceptors. Mol Biol Cell. 30, 2890-2900 (2019)(PMID:31553680)
  4. Watanabe K et al. The fornix acts as a permissive corridor for septal neuron migration beyond the diencephalic-telencephalic boundary. Sci Rep. 10, 8315 (2020)(PMID:32433594)

ELISA

  1. Sangawa T et al. A multipurpose fusion tag derived from an unstructured and hyperacidic region of the amyloid precursor protein. Protein Sci. 22, 840-50 (2013)(PMID:23526492)
Product category
Tools
Epitope tags
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  • The availability is based on the information in Japan at 17:00, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.