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HOME > Product search results > Code No. RN014MW Anti-SFPQ (PSF) mAb

Code No. RN014MW

Anti-SFPQ (PSF) mAb

Price

¥48,000

Availability (in Japan)

1-9

(In Japan at 17:00,
Sep 15, 2019 in JST)

Size

100 µg/100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

  • Immunocytochemistry

  • Immunohistochemistry

Clonality Monoclonal Clone C23
Isotype (Immunized Animal) Mouse IgG2aκ
Applications
WB
1 µg/mL  
IP
2 µg/sample  
IC
0.5 µg/mL  
IH
1 µg/mL  
Immunogen (Antigen) KLH-conjugated synthetic peptide, CAGYGRGREEYEGPNKKPRF (corresponding to amino acid residues 681-699 of mouse Sfpq)
Reactivity [Gene ID]

Human[6421], Mouse[71514], Rat[252855], Hamster[100761355]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names POMP100, PPP1R140
Background Paraspeckle is one of newly identified subnuclear bodies consists of RNAs and RNA-binding proteins. SFPQ, also known as PSF, is a core component of paraspeckle and belongs to DBHS protein family. SFPQ forms heteromer by binding to other DHBS protein family, such as p54/NONO, and participates in mRNA splicing and transcriptional regulation in the nucleus. In Alzheimer's and Pick's diseases known as tauopathy, SFPQ is accumulated in cytoplasm in neurons and astrocytes. Altered localization of SFPQ together with tau mutation may be involved in pathogenesis of neurodegenerative diseases.
Related products RN013MW Anti-Nono (P54NRB) mAb
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M041-3 Anti-PML (Human) mAb
PM064 Anti-Lamin B1 pAb
PM091 Anti-Per1 (Mouse) pAb
PM093 Anti-NR1D2 (Rev-erbβ) pAb
Citations

Western Blotting

  1. Yamazaki T et al. Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation. Mol Cell. 70, 1038-1053.e7. (2018)(PMID:29932899)

Immunocytochemistry

  1. Yamazaki T et al. Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation. Mol Cell. 70, 1038-1053.e7. (2018)(PMID:29932899)
References
  1. Imamura K et al. Long noncoding RNA NEAT1-dependent SFPQ relocation from promoter region to paraspeckle mediates IL8 expression upon immune stimuli. Mol Cell 53, 393-406 (2014) (PMID:24507715)
  2. Hirose T et al. NEAT1 long noncoding RNA regulates transcription via protein sequestration within subnuclear bodies. Mol Biol Cell 25,169-83 (2014) (PMID:24173718)
  3. Elzbieta K et al. Distinct roles of DBHS family members in the circadian transcriptional feedback loop. Mol Cell Biol. 32, 4585-94 (2012) (PMID:22966205)
  4. Nakagawa S, Hirose T. Paraspeckle nuclear bodies--useful uselessness? Cell Mol Life Sci. 69, 3027–36 (2012) (PMID:22476590)
  5. Nakagawa S et al. Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice. J Cell Biol. 193, 31-9 (2011) (PMID:21444682)
  6. Kuwahara S et al. PSPC1, NONO, and SFPQ are expressed in mouse Sertoli cells and may function as coregulators of androgen receptor-mediated transcription. Biol Reprod. 75, 352-9 (2006) (PMID:16641145)
  7. Fox AH et al. Paraspeckles: a novel nuclear domain. Curr Biol. 12, 13-25 (2002) (PMID:11790299)
Product category
Research area
RNA-RNP Network
Epigenetics
Brands

RiboCluster Profiler™
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  • The availability is based on the information in Japan at 17:00, Sep 15, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.