Anti-Atg7 (Human) pAb

Price

¥41,800

Availability (in Japan)

1-9

（In Japan at 17:00,
Apr 18, 2024 in JST）

Size

100 µL

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation
Clonality	Polyclonal	Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB 1:1,000-1:2,000 IP 5 µL/300 µL of cell extract from 3x10⁶ cells
Immunogen (Antigen)	Human Atg7 C-terminal synthetic peptide
Reactivity [Gene ID]	Human[10533], Mouse(-), Rat(-), Hamster(-)
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	autophagy related 7, GSA7, APG7L, APG7-LIKE
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Microtubule-associated protein light chain 3 (LC3) is a homologue of yeast Atg8, an essential component of autophagy. Following synthesis, the C-terminus of LC3 is cleaved by a cysteine protease-Atg4, to produce LC3-I, which is located in cytosolic fraction. LC3-I is activated by the E1-like enzyme Atg7 and forms a Atg7-LC3-I thioester. Atg7-LC3-I is transferred to Atg3 to form Atg3-LC3-I thioester. Atg3 is an E2-like enzyme that catalyzes the conjugation of LC3-I and phosphatidylethanolamine (PE) to form LC3-II. The LC3-II-PE conjugate is essential for binding tightly to autophagosomal membrane.
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Citations	Western Blotting Fujita K et al. Nrf2-mediated induction of p62 controls Toll-like receptor-4-driven aggresome-like induced structure formation and autophagic degradation. PNAS. 108, 1427-32 (2011)(PMID:21220332) Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013)(PMID:24141421) Horikawa I et al. Autophagic degradation of the inhibitory p53 isoform Δ133p53α as a regulatory mechanism for p53-mediated senescence. Nat Commun. 5, 4706 (2014)(PMID:25144556) Xu D et al. Modification of BECN1 by ISG15 plays a crucial role in autophagy regulation by type I IFN/interferon. Autophagy 11, 617-28 (2015)(PMID:25906440) He R et al. Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy 11, 740-7 (2015)(PMID:25955014) Dutta P et al. MIR494 reduces renal cancer cell survival coinciding with increased lipid droplets and mitochondrial changes. BMC Cancer 16, 33 (2016)(PMID:26794413) Kim HJ et al. Subcellular localization of FOXO3a as a potential biomarker of response to combined treatment with inhibitors of PI3K and autophagy in PIK3CA-mutant cancer cells. Oncotarget. 8, 6608-6622 (2017)(PMID:28036259) He R et al. Glucosyltransferase Activity of Clostridium difficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep. 7, 10532 (2017)(PMID:28874882) Liu WJ et al. Lysosome restoration to activate podocyte autophagy: a new therapeutic strategy for diabetic kidney disease. Cell Death Dis. 10, 806 (2019)(PMID:31649253) Chhabra R, Nanjundan M. Lysophosphatidic acid reverses Temsirolimus-induced changes in lipid droplets and mitochondrial networks in renal cancer cells. PLoS One.15, e0233887 (2020)(PMID:32492043) Immunofluorescence Liu WJ et al. Lysosome restoration to activate podocyte autophagy: a new therapeutic strategy for diabetic kidney disease. Cell Death Dis. 10, 806 (2019)(PMID:31649253)
Product category	Research area Autophagy

The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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