Anti-TERT (Human) mAb

Price

¥74,800

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting ChIP Immunoprecipitated DNA was analyzed by dot blotting using telomere restriction fragment (TRF) probe.
Clonality	Monoclonal	Clone	10E9-2
Isotype (Immunized Animal)	Mouse IgG2bκ
Applications	WB 0.5-10 µg/mL （PMID: 24550003） IP* reported. （PMID: 24550003 / 26830230） IC* reported. （PMID: 24550003） ChIP* Can be used. （PMID: 24550003）
Immunogen (Antigen)	Recombinant C-terminal His-tagged hTERT protein of 157 amino acids of hTERT (position 304 to 460)
Reactivity [Gene ID]	Human[7015](PMID: 24550003)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	TP2, TRT, CMM9, EST2, TCS1, hTRT, DKCA2, DKCB4, PFBMFT1
Background	Human telomerase reverse transcriptase (hTERT) is 1132 amino acids, 127 kDa protein, that is the catalytic subunit of the telomerase. Telomerase is an RNA-dependent DNA polymerase that catalyzes the addition of telomeric repeat unit, 5'-TTAGGG-3', to chromosome ends. Human telomerase consists of three major subunits: TERT, a template RNA called hTR, and telomerase-associated protein (TEP-1). hTERT and hTR are minimally required to reconstitute telomerase activity in vitro. In these subunits, hTR is constitutively expressed; TERT transcription is a primary mechanism for regulation of telomerase activity. In most human somatic cells, telomerase activity is undetectable, whereas approximately 85% of human cancers, including breast, prostate, stomach, bladder, colon, and liver cancer, have telomerase activity. Activation of telomerase is implicated in cell immortalization and cancer cell pathogenesis.
Related products	M077-3 Mouse IgG2b (isotype control)
Citations	Western Blotting Maida Y et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 34, 1576-93 (2014)(PMID:24550003) ChIP Maida Y et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 34, 1576-93 (2014)(PMID:24550003) Immunoprecipitation Maida Y et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 34, 1576-93 (2014)(PMID:24550003) Maida Y et al.De Novo RNA Synthesis by RNA-Dependent RNA Polymerase Activity of Telomerase Reverse Transcriptase. Mol Cell Biol. 36, 1248-59 (2016)(PMID:26830230) Takahashi M et al. Eribulin penetrates brain tumor tissue and prolongs survival of mice harboring intracerebral glioblastoma xenografts. Cancer Sci. 110, 2247-2257 (2019)(PMID:31099446) Immunocytochemistry Maida Y et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 34, 1576-93 (2014)(PMID:24550003)
Product category	Research area Epigenetics Cancer

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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