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HOME > Product search results > Code No. M152-3 Anti-LC3 mAb

Code No. M152-3

Anti-LC3 mAb

Price

¥48,000

Availability (in Japan)

10 or more

(In Japan at 01:30,
Oct 24, 2017 in JST)

Size

200 µg/100 µL

Data
  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

  • Other

Clonality Monoclonal Clone 4E12
Isotype (Immunized Animal) Mouse IgG1 κ
Applications
WB
5 µg/mL  
IP
5 µg/300 µL of cell extract from 1x107 cells  
FCM
40 µg/mL  
IC
40 µg/mL  
IH*
reported.  (PMID: 21957238 / 19955365 / 19915056
Other(Immuno-EM)
20 µg/mL  (PMID: 24553140
Other(Image-based flow cytometry)
reported.  (PMID: 24553140
Immunogen (Antigen) Recombinant Human LC3 (MAP1LC3B: 1-120 a.a.)
Reactivity [Gene ID]

Human[81631/84557], Mouse[67443/66734], Rat[64862/362245], Hamster[100760576/100769810]

Storage buffer 2 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, MAP1LC3A
Background Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.
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Notes Clone 4E12 reacts with LC3 (MAP1LC3A and MAP1LC3B).
Citations
  1. Wang Z et al. Autophagy regulates myeloid cell differentiation by p62/SQSTM1-mediated degradation of PML-RARα oncoprotein. Autophagy. 7, 401-11 (2011)(PMID:21187718)
  1. Hartleben B et al. Autophagy influences glomerular disease susceptibility and maintains podocyte homeostasis in aging mice. J Clin Invest. 120, 1084-96 (2010)(PMID:20200449)

Western Blotting

  1. Frankel LB et al. microRNA-101 is a potent inhibitor of autophagy. EMBO J. 30, 4628-41 (2011)(PMID:21915098)
  2. Starr T et al. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host Microbe. 11, 33-45 (2012)(PMID:22264511)
  3. Shi CS et al. Activation of autophagy by inflammatory signals limits IL-1β production by targeting ubiquitinated inflammasomes for destruction. Nat Immunol. 13, 255-63 (2012)(PMID:22286270)
  4. Morris CR et al. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. PLoS One. 7, e34308 (2012)(PMID:22479596)
  5. Ho H et al. WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. J Biol Chem. 286, 12509-23 (2011)(PMID:21317285)
  6. Saiki S et al. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy. 7, 176-87 (2011)(PMID:21081844)

Flow Cytometry

  1. Eng KE et al. A novel quantitative flow cytometry-based assay for autophagy. Autophagy. 6, 634-41 (2010)(PMID:20458170)

Immunocytochemistry

  1. Moreau K et al. Autophagosome precursor maturation requires homotypic fusion. Cell. 146, 303-17 (2011)(PMID:21784250)
  2. Gillis JM et al. Aminopeptidase-resistant peptides are targeted to lysosomes and subsequently degraded. Traffic. 12, 1897-910 (2011)(PMID:21883763)
  3. Seto S et al. Coronin-1a inhibits autophagosome formation around Mycobacterium tuberculosis-containing phagosomes and assists mycobacterial survival in macrophages. Cell Microbiol. 14, 710-27 (2012)(PMID:22256790)
  4. Starr T et al. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host Microbe. 11, 33-45 (2012)(PMID:22264511)
  5. Sims JJ et al. Polyubiquitin-sensor proteins reveal localization and linkage-type dependence of cellular ubiquitin signaling. Nat Methods. 9, 303-9 (2012)(PMID:22306808)
  6. McKnight NC et al. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. EMBO J. 31, 1931-46 (2012)(PMID:22354037)
  7. Morris CR et al. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. PLoS One. 7, e34308 (2012)(PMID:22479596)
  8. Joubert PE et al. Chikungunya virus-induced autophagy delays caspase-dependent cell death. J Exp Med. 209,1029-47 (2012)(PMID:22508836)
  9. Traver MK et al. Immunity-related GTPase M (IRGM) proteins influence the localization of guanylate-binding protein 2 (GBP2) by modulating macroautophagy. J Biol Chem. 286, 30471-80 (2011)(PMID:21757726)
  10. Cai T et al. Deletion of Ia-2 and/or Ia-2β in mice decreases insulin secretion by reducing the number of dense core vesicles. Diabetologia. 54, 2347-57 (2011)(PMID:21732083)
  11. Kovacs JR et al. Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery. Cell Death Differ. 19, 144-52 (2012)(PMID:21660048)
  12. Thayanidhi N et al. Alpha-synuclein delays endoplasmic reticulum (ER)-to-Golgi transport in mammalian cells by antagonizing ER/Golgi SNAREs. Mol Biol Cell. 21, 1850-63 (2010)(PMID:20392839)

Immunohistochemistry

  1. Chalazonitis A et al. Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. J Neurosci. 31, 13746-57 (2011)(PMID:21957238)
Product category
Research area
Autophagy
Brands
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  • The availability is based on the information in Japan at 01:30, Oct 24, 2017 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.