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HOME > Product search results > Code No. M084-3 Anti-Podocalyxin (PCLP1) (Human) mAb

Code No. M084-3

Anti-Podocalyxin (PCLP1) (Human) mAb

Price

¥48,000

Availability (in Japan)

1-9

(In Japan at 00:05,
Aug 25, 2019 in JST)

Size

100 µg/100 µL

Data
  • Flow Cytometry

Clonality Monoclonal Clone 53D11
Isotype (Immunized Animal) Mouse IgG2a
Applications
FCM
10-20 µg/mL (final concentration)  
Immunogen (Antigen) CHO cells expressing full-length human Podocalyxin/PCLP1
Reactivity [Gene ID]

Human[5420]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names PODXL, podocalyxin like, PC, PCLP, Gp200, PCLP-1
Background Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. Hara et al. molecularly cloned podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the AGM (aorta-gonad-mesonephros) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1+ CD45- cells in the AGM region incorporated acetylated low-density lipoprotein and produced both hematopoietic and endothelial cells when co-cultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1+ CD45- cells were injected into neonatal liver of busulfan-treated mice. Today it is reported that the PCLP1 is identical with the Podocalyxin.
Related products D340-3 Anti-SM22α mAb
M084-4 Anti-Podocalyxin (PCLP1) (Human) mAb-FITC
M084-5 Anti-Podocalyxin (PCLP1) (Human) mAb-PE
M076-3 Mouse IgG2a (isotype control)
Citations

Flow Cytometry

  1. Itai S et al. Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma. Oncotarget 9, 22480-22497 (2018)(PMID:29854293)
References
  1. Hara T et al. Identification of podocalyxin-like protein 1 as a novel cell surface marker for hemangioblasts in the murine aorta-gonad-mesonephros region. Immunity 11, 567-78 (1999)(PMID: 10591182
Product category
Research area
Stem cell research
Cell surface antigens
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 00:05, Aug 25, 2019 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.