Anti-Caspase-7 mAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Western Blotting Immunoprecipitation
Clonality	Monoclonal	Clone	4G2
Isotype (Immunized Animal)	Mouse IgG2b
Applications	WB 1 µg/mL IP 2 µg/200 µL of cell extract from 2.5 x 10⁶ cells
Immunogen (Antigen)	Recombinant Caspase-7 (N-terminal :1-198 a.a.)
Reactivity [Gene ID]	Human[840], Mouse[12369], Rat[64026]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	CASP7
Background	Apoptosis is a major form of cell death characterized by several morphological features that include chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. These morphological changes occur via signaling pathway that leads to the recruitment and activation of caspases, a family of cysteine-containing, aspartate-specific proteases. Caspases exist as inactive proenzymes in cells and are activated through their processing into two subunits in response to apoptotic stimulation. Activated caspases cleave a variety of important cellular proteins, other caspases, and Bcl-2 family members, leading to a commitment to cell death. Caspase-7 (also known as Mch-3 / ICE-LAP3 / CMH-1) is a 35 kDa protein that has the highest similarity to caspase-3 (52% amino acid identity) between all caspase members. It has been involved in ''effector'' caspases (which include caspase 3, 6, 7) that are cleaved by ''initiator'' caspases (which include caspase 8, 9) into active form, and then, in turn cleave various cellular proteins for apoptosis. Recent study says that in Fas mediated hepatocyte apoptosis, active caspase-7 is associated almost exclusively with the mitochondrial and microsomal fractions, whereas active caspase-3 is confined primarily to the cytosol. It implies the different role of caspase-3 and -7 in the execution of apoptosis.
Related products	M032-3 Anti-Caspase-8 (Human) mAb M058-3 Anti-Caspase-8 (Human) mAb M029-3 Anti-Caspase-4 (Human) mAb M044-3 Anti-XIAP (MIHA/ILP-a) mAb M087-3 Anti-Caspase-3 (Human) mAb M097-3 Anti-Caspase-3 (Human) mAb
Citations	Western Blotting Li M et al. Clearance of HIV infection by selective elimination of host cells capable of producing HIV. Nat Commun. 11, 4051 (2020) (PMID:32792548)
Product category	Research area Apoptosis

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.