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HOME > Product search results > Code No. M019-3 Anti-Nucleolin mAb

Code No.　M019-3

Anti-Nucleolin mAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 18, 2024 in JST）

Size

100 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunohistochemistry
Clonality Monoclonal Clone 4E2
Isotype (Immunized Animal) Mouse IgG1
Applications
WB
1 µg/mL  
IP*
reported.  （PMID: 12185196 / 12938232）
FCM
  
IC
1-10 µg/mL  
IH
1-10 µg/mL  
Other(inhibition for calcium oxalate monohydrate crystal binding)
reported.  （PMID: 12538728）
Immunogen (Antigen) human nucleolin from Raji cell extract
Reactivity [Gene ID]

Human[4691], Mouse(-), Rat(-), Bovine*[497013](PMID: 14613906), Monkey*[103218056](PMID: 12642096), Dog*[608445](PMID: 12538728/15100100)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names NCL, C23
Background Nucleolin is a major nucleolar phosphoprotein which is associated with preribosomal RNA and is implicated in the early stage of preribosomal RNP assembly and processing. This 100 kDa protein can be subdivided into three major domains: The N-terminal domain comprises long acidic stretches interspersed with basic repeats, similar to the structure of a high mobility group-type protein. This domain is responsible for the ability of nucleolin to modulate chromatin condensation. In contrast, the central domain contains four RNA binding elements. Finally, the C-terminal domain, approximately 85 amino acids long, is strikingly rich in glycine, arginine, and phenylalanine residues. Nucleolin fluctuates in parallel to DNA synthesis. The intact 100 kDa nucleolin molecule is the major species in actively dividing cells, whereas the degraded forms are relatively abundant in nondividing cells. Stability of nucleolin molecule is cell proliferation-dependent. Nucleolin is found to be identical to the human DNA helicase IV. Nucleolin/DNA helicase IV can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Furthermore, nucleolin directly interacts with DNA topoisomerase I.
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Citations

Western Blotting

  1. Bates PJ et al. Antiproliferative activity of G-rich oligonucleotides correlates with protein binding. J Biol Chem. 274, 26369-77 (1999)(PMID:10473594)
  2. Morimoto H et al. Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells. J Histochem Cytochem. 50, 1187-93 (2002)(PMID:12185196)
  3. Watson WH, Jones DP. Oxidation of nuclear thioredoxin during oxidative stress. FEBS Lett. 543, 144-7 (2003)(PMID:12753922)
  4. Barel M et al. Activation of Epstein-Barr virus/C3d receptor (gp140, CR2, CD21) on human cell surface triggers pp60src and Akt-GSK3 activities upstream and downstream to PI 3-kinase, respectively. Eur J Immunol. 33, 2557-66 (2003)(PMID:12938232)
  5. Gueven N et al. Aprataxin, a novel protein that protects against genotoxic stress. Hum Mol Genet. 13, 1081-93 (2004)(PMID:15044383)
  6. Bose S et al. Role of nucleolin in human parainfluenza virus type 3 infection of human lung epithelial cells. J Virol. 78, 8146-58 (2004)(PMID:15254186)
  7. Sorokina EA et al. An acidic peptide sequence of nucleolin-related protein can mediate the attachment of calcium oxalate to renal tubule cells. J Am Soc Nephrol. 15, 2057-65 (2004)(PMID:15284292)
  8. Kawauchi K et al. IL-2 increases human telomerase reverse transcriptase activity transcriptionally and posttranslationally through phosphatidylinositol 3'-kinase/Akt, heat shock protein 90, and mammalian target of rapamycin in transformed NK cells. J Immunol. 174, 5261-9 (2005)(PMID:15843522)
  9. Sakaguchi M et al. Bifurcated converging pathways for high Ca2+- and TGFbeta-induced inhibition of growth of normal human keratinocytes. PNAS. 102, 13921-6 (2005)(PMID:16172401)
  10. Wu YL et al. Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths. J Cell Sci. 119, 2797-806 (2006)(PMID:16772337)
  11. Becherel OJ et al. Nucleolar localization of aprataxin is dependent on interaction with nucleolin and on active ribosomal DNA transcription. Hum Mol Genet. 15, 2239-49 (2006)(PMID:16777843)
  12. Shiohama A et al. Nucleolar localization of DGCR8 and identification of eleven DGCR8-associated proteins. Exp Cell Res. 313, 4196-207 (2007)(PMID:17765891)
  13. Kitao S et al. A compartmentalized phosphorylation/dephosphorylation system that regulates U snRNA export from the nucleus. Mol Cell Biol. 28, 487-97 (2008)(PMID:17967890)
  14. Pickering B et al. Nucleolin protein interacts with microprocessor complex to affect biogenesis of microRNAs 15a and 16. J Biol Chem. 286, 44095-103 (2011)(PMID:22049078)
  15. Hayashi Y et al. The hierarchical structure of the perichromosomal layer comprises Ki67, ribosomal RNAs, and nucleolar proteins. Biochem Biophys Res Commun. 493, 1043-1049 (2017)(PMID:28935370)

Immunoprecipitation

  1. Morimoto H et al. Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells. J Histochem Cytochem. 50, 1187-93 (2002)(PMID:12185196)
  2. Barel M et al. Activation of Epstein-Barr virus/C3d receptor (gp140, CR2, CD21) on human cell surface triggers pp60src and Akt-GSK3 activities upstream and downstream to PI 3-kinase, respectively. Eur J Immunol. 33, 2557-66 (2003)(PMID:12938232)
  3. Wu YL et al. Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths. J Cell Sci. 119, 2797-806 (2006)(PMID:16772337)

Immunocytochemistry

  1. Takagi M et al. Chmadrin: a novel Ki-67 antigen-related perichromosomal protein possibly implicated in higher order chromatin structure. J Cell Sci. 112, 2463-72 (1999)(PMID:10393802)
  2. Morimoto H et al. Interaction of protein phosphatase 1 delta with nucleolin in human osteoblastic cells. J Histochem Cytochem. 50, 1187-93 (2002)(PMID:12185196)
  3. Guo YX et al. Identification of nucleolus localization signal of betanodavirus GGNNV protein alpha. Virology. 306, 225-35 (2003)(PMID:12642096)
  4. Baran V et al. Immunolocalization of upstream binding factor and pocket protein p130 during final stages of bovine oocyte growth. Biol Reprod. 70, 877-86 (2004)(PMID:14613906)
  5. Gueven N et al. Aprataxin, a novel protein that protects against genotoxic stress. Hum Mol Genet. 13, 1081-93 (2004)(PMID:15044383)
  6. Kumar V et al. Renal epithelial cells constitutively produce a protein that blocks adhesion of crystals to their surface. Am J Physiol Renal Physiol. 287, F373-83 (2004)(PMID:15100100)
  7. Sorokina EA et al. An acidic peptide sequence of nucleolin-related protein can mediate the attachment of calcium oxalate to renal tubule cells. J Am Soc Nephrol. 15, 2057-65 (2004)(PMID:15284292)
  8. Becherel OJ et al. Nucleolar localization of aprataxin is dependent on interaction with nucleolin and on active ribosomal DNA transcription. Hum Mol Genet. 15, 2239-49 (2006)(PMID:16777843)
  9. Zhu CH et al. SGNP: an essential Stress Granule/Nucleolar Protein potentially involved in 5.8s rRNA processing/transport. PLoS One. 3, e3716 (2008)(PMID:19005571)
  10. Gorsler T et al. DNA damage-induced translocation of S100A11 into the nucleus regulates cell proliferation. BMC Cell Biol. 11, 100 (2010)(PMID:21167017)
  11. Uozumi N et al. Detection of O-propargyl-puromycin with SUMO and ubiquitin by click chemistry at PML-nuclear bodies during abortive proteasome activities. Biochem Biophys Res Commun. 474, 247-251 (2016)(PMID:27125456)
  12. Kadono M et al. Biological implications of somatic DDX41 p.R525H mutation in acute myeloid leukemia. Exp Hematol. 44, 745-754 (2016)(PMID:27174803)
  13. Hayashi Y et al. Nucleolar integrity during interphase supports faithful Cdk1 activation and mitotic entry. Sci Adv. 4, eaap7777 (2018)(PMID:29881774)

Immunohistochemistry

  1. Osawa N et al. Diagnosis of parathyroid carcinoma using immunohistochemical staining against hTERT. Int J Mol Med. 24, 733-41 (2009)(PMID:19885612)

Other(inhibition for calcium oxalate monohydrate crystal binding)

  1. Kumar V et al. Annexin II is present on renal epithelial cells and binds calcium oxalate monohydrate crystals. J Am Soc Nephrol. 14, 289-97 (2003)(PMID:12538728)
Product category
Research area
RNA-RNP Network
Epigenetics
Cancer
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  • The availability is based on the information in Japan at 17:00, Apr 18, 2024 in JST.
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  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.