Anti-Ace (CD143) (Mouse) mAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Data Sheet

SDS

This antibody (clone 1D5) reacts with both somatic and testis isoforms of Ace (PMID: 16870943). Useful for Western blotting and Immunohistochemistry.

Data	Western Blotting Immunohistochemistry
Clonality	Monoclonal	Clone	1D5
Isotype (Immunized Animal)	Mouse IgG2bκ
Applications	WB 1 µg/mL IH 1 µg/mL (Heat treatment is necessary for paraffin embedded sections.)
Immunogen (Antigen)	Recombinant protein corresponding to N-terminal region of mouse tACE (testicular ACE) without the signal peptide
Reactivity [Gene ID]	Mouse[11421]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	angiotensin I converting enzyme (peptidyl-dipeptidase A) 1, DCP, ACE1, DCP1
Background	Angiotensin Converting Enzyme（ACE; アンジオテンシン変換酵素）は、Angiotensin I を活性体であるAngiotensin IIに変換することによって血圧を制御する触媒酵素であり、細胞膜上に存在するタンパク質である。ACEには全身の細胞に広く発現するsomatic ACE（sACE）と、限られた組織にのみ発現するgerminal ACE（gACE）が存在し、gACEは精巣に発現していることが知られている。本モノクローナル抗体（クローン 1D5は、マウス精巣のACE（testicular ACE）を抗原として作製された。Testis-specific isozymeであるtACEは、未成熟精子細胞と成熟した精子の両方に発現していて、受精の際に精子の透明帯結合に関わる分子の一つである。 Calmegin、ADAM1、ADMA2、ADAM3とともにノックアウトすると透明帯に結合できなくなることが知られている。
Related products	M207-3 Anti-MitoPLD (Pld6) mAb PM044 Anti-PIWIL2 (MILI) (Mouse) pAb D356-3 Anti-Jmjd1c (Mouse) mAb M077-3 Mouse IgG2b (isotype control) D363-3 Anti-Ly6k (Mouse) mAb D365-3 Anti-PIWIL2 (MILI) (Mouse) mAb
References	Hagaman JR et al. Angiotensin-converting enzyme and male fertility. PNAS. 95, 2552-7 (1998)（PMID: 9482924） Yamaguchi R et al. Aberrant distribution of ADAM3 in sperm from both angiotensin-converting enzyme (Ace)- and calmegin (Clgn)-deficient mice. Biol Reprod. 75, 760-6 (2006)（PMID: 16870943） Deguchi E et al. Dipeptidase-inactivated tACE action in vivo: selective inhibition of sperm-zona pellucida binding in the mouse. Biol Reprod. 77, 794-802 (2007)（PMID: 17634445） Fujihara Y et al. Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa. PNAS 110, 8111-6 (2013)（PMID: 23633567） Fujihara Y et al. GPI-anchored protein complex, LY6K/TEX101, is required for sperm migration into the oviduct and male fertility in mice. Biol Reprod. 90, 60 (2014)（PMID: 24501175）
Product category	Research area Stem cell research Cell surface antigens

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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