Anti-5-methylcytidine (m⁵C) mAb

Price

¥66,000

Availability (in Japan)

1-9

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Anti-5-methylcytidine (m⁵C) mAb (Clone: FMC-9) reacts with 5-methylcytidine (m⁵C or m5Cyd), 5-methyl-2’-deoxycytidine (m5dC or m5dCyd) and 5-methylcytosine (5mC, 5-mC or m5Cyt).

Data	Immunoprecipitation MeDIP was performed using mouse genomic DNA fragments containing synthesized DNAs. IP'd samples were analyzed by real-time qPCR using primers specific to synthetic DNA spike-in controls or Loci 1-3. Immunocytochemistry Immunohistochemistry Immunohistochemistry Dot Blotting
Clonality	Monoclonal	Clone	FMC-9
Isotype (Immunized Animal)	Mouse IgG2aλ
Applications	IP Can be used. IC 5 µg/mL (for cytoplasmic staining), 1 µg/mL (for nuclear staining, HCl-treated) IH 10 µg/mL (For nuclear staining, heat treatment or HCl treatment is necessary.) ELISA Inhibition ELISA (Info. from the licenser. Not tested by MBL.) DB Can be used. Other(Immuno-Nothern blotting) Can be used. （PMID: 26606401）
Immunogen (Antigen)	KLH-conjugated 5-methylcytidine (KLH-m⁵C)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	m5Cyd
Background	5-Methylcytosine (5mC), a modified nucleobase derived from cytosine, is present in both DNA and RNA molecules. 5mC in DNA is an essential epigenetic mark associated with gene silencing and genome stability during embryonic development. 5mC in ribonucleoside, called 5-methylcytidine (m⁵C), is involved in structural stability, aminoacylation and codon recognition of tRNA. In rRNA, m⁵C affects translational fidelity and tRNA recognition. m⁵C-modified nucleobase also have been identified in mRNA and other non-coding RNA but the biological functions are still unclear. m⁵C is detected not only in tissues and cells but also in urine. It is reported that the levels of m⁵C change in urine or tissues from patients with various diseases such as bladder, breast and colon cancers.
Related products	D345-3 Anti-1-methyladenosine (m¹A) mAb PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb MI-11-3 Anti-Bromodeoxyuridine mAb M076-3 Mouse IgG2a (isotype control) PM085 Anti-ZBTB24 pAb PM086 Anti-ZBTB24 (Human) pAb D347-3 Anti-Pseudouridine mAb M227-3 Anti-Digoxigenin (DIG) mAb M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb M228-3 Anti-FITC mAb RN019M Anti-2,2,7-trimethylguanosine (m₃G/TMG) mAb RN016M Anti-7-methylguanosine (m⁷G)-Cap mAb RN017M Anti-7-methylguanosine (m⁷G) mAb D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb M202-3 Anti-Np95 (Uhrf1) mAb RN127PW Anti-NSUN2 (Human) pAb PM098 Anti-Inosine pAb
Citations	Dot Blotting Garvilles RG et al. Dual Functions of the RFTS Domain of Dnmt1 in Replication-Coupled DNA Methylation and in Protection of the Genome from Aberrant Methylation. PLoS One 10, e0137509 (2015)(PMID:26383849) Immunofluorescence Totsuka T et al. Production of mouse androgenetic embryos using spindle perturbation. Sci Rep. 10, 6556 (2020)(PMID:32300113) Other(Immuno-Nothern blotting) Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
References	Seki Y et al. Extensive and orderly reprogramming of genome-wide chromatin modifications associated with specification and early development of germ cells in mice. Dev Biol. 278, 440-58 (2005)（PMID: 15680362） Sakai Y et al. Expression of DNA methyltransferase (Dnmt1) in testicular germ cells during development of mouse embryo. Cell Struct Funct. 26, 685-91 (2001)（PMID: 11942627） Mizugaki M et al. Preparation of a monoclonal antibody specific for 5-methyl-2'-deoxycytidine and its application for the detection of DNA methylation levels in human peripheral blood cells. Biol Pharm Bull. 19, 1537-40 (1996)（PMID: 8996634）
Product category	Research area RNA-RNP Network Epigenetics

The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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