Anti-Mincle (Mouse) mAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Data	Immunoprecipitation Flow Cytometry
Clonality	Monoclonal	Clone	4A9
Isotype (Immunized Animal)	Rat IgG1 κ
Applications	WB 1-5 µg/mL IP 5-10 µg/200 µL cell extract from 1 x 10⁶ cells FCM 5-10 µg/mL (final concentration) Function 1-10 µg/mL (final concentration) （PMID: 18776906）
Immunogen (Antigen)	Mouse Mincle (full length) transfected RBL-2H3 cells
Reactivity [Gene ID]	Mouse[56619]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	Clec4e, C-type lectin domain family 4, member e, C86253, Clecsf9
Background	Macrophage-inducible C-type lectin (Mincle, also called as Clec4e and Clecsf9), a type II transmembrane C-type lectin receptor, is expressed mainly in macrophages. Mincle is selectively associated with the Fc receptor common g-chain which contains immunoreceptor tyrosine-based activation motif (ITAM) and activates macrophages to produce inflammatory cytokines and chemokines. Mincle expressing cells are activated by spliceosome associated protein 130 (SAP130) which is a component of small nuclear ribonucleoprotein released from dead cells. Recently, Mincle is reported to recognize Mycobacterium tuberculosis as well as pathogenic fungus Malassezia species. Mincle is demonstrated to be an essential receptor for a mycobacterial glycolipid, trehalose-6,6’-dimycolate (TDM). The cytokine/chemokine production of macrophages which Malassezia or M.tuberculosis induces in Mincle-/- mice is significantly impaired. Mincle is considered to play a crucial role in immune responses to pathogens.
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Citations	Western Blotting Mohanraj M et al. The Mycobacterial Adjuvant Analogue TDB Attenuates Neuroinflammation via Mincle-Independent PLC-γ1/PKC/ERK Signaling and Microglial Polarization. Mol Neurobiol. (2018) In press.(PMID:29876879) Tan RZ et al. Curcumin relieved cisplatin-induced kidney inflammation through inhibiting Mincle-maintained M1 macrophage phenotype. Phytomedicine 52, 284-294 (2019)(PMID:30599909) Flow Cytometry Miyake Y et al. C-type Lectin MCL Is an FcR?A-Coupled Receptor that Mediates the Adjuvanticity of Mycobacterial Cord Factor. Immunity. 38, 1050-62 (2013)(PMID:23602766) Toyonaga K et al. Characterization of the receptors for mycobacterial cord factor in Guinea pig. PLoS One 9, e88747 (2014)(PMID:24533147) Mohanraj M et al. The Mycobacterial Adjuvant Analogue TDB Attenuates Neuroinflammation via Mincle-Independent PLC-γ1/PKC/ERK Signaling and Microglial Polarization. Mol Neurobiol. (2018) In press.(PMID:29876879) Fites JS et al. An unappreciated role for neutrophil-DC hybrids in immunity to invasive fungal infections. PLoS Pathog. 14, e1007073 (2018)(PMID:29782541) Function Yamasaki S et al. Mincle is an ITAM-coupled activating receptor that senses damaged cells. Nat Immunol. 9, 1179-88 (2008)(PMID:18776906)
Product category	Research area Immunology

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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