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HOME > Product search results > Code No. D221-3 Anti-Sap155 mAb

Code No.　D221-3

Anti-Sap155 mAb

Price

¥52,800

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 19, 2024 in JST）

Size

100 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry
Clonality Monoclonal Clone 16
Isotype (Immunized Animal) Mouse IgG2b
Applications
WB
1 µg/mL  
IP
1 µg/200 µL of cell extract from 5x106 cells  
IC
10 µg/mL  
Immunogen (Antigen) Recombinant Sap155 (98-198 a.a.)
Reactivity [Gene ID]

Human[23451](PMID: 18842582), Mouse[81898]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names SF3B1, MDS, PRP10, Hsh155, PRPF10, SF3b155/TA-8, Prp10, Targ4, SAP155, SF3b155
Background SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the major spliceosome. SF3 contains the Spliceosome-Associated Proteins, Sap 49, 130, 145, and 155. Sap155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) spliceosome. Sap155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two-hybrid system. Sap155 contains numerous Cdk consensus phosphorylation sites in its N-terminus and is phosphorylated prior to catalytic step II of the splicing pathway. Sap155 serves as a substrate for cyclin E-cdk2 in vitro, suggesting that pre-mRNA splicing may be linked to the cell cycle machinery in mammalian cells.
Related products D138-3 Anti-Sap155 mAb
D139-3 Anti-Ring1B mAb
RN085PW Anti-U2AF1 pAb
Citations

Western Blotting

  1. Kotake Y et al. Splicing factor SF3b as a target of the antitumor natural product pladienolide. Nat Chem Biol. 3, 570-5 (2007)(PMID:17643112)
  2. Yamasaki S et al. Mincle is an ITAM-coupled activating receptor that senses damaged cells. Nat Immunol. 9, 1179-1188 (2008)(PMID:18776906)
  3. Tanuma N et al. Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1 (PP1) to dephosphorylate the U2 small nuclear ribonucleoprotein particle (snRNP) component, spliceosome-associated protein 155 (Sap155). J Biol Chem. 283, 35805-14 (2008)(PMID:18842582)
  4. Yokoi A et al. Biological validation that SF3b is a target of the antitumor macrolide pladienolide. FEBS J. 278, 4870-80 (2011)(PMID:21981285)
  5. Takemura R et al. Multiple factors in the early splicing complex are involved in the nuclear retention of pre-mRNAs in mammalian cells. Genes Cells. 16, 1035-49 (2011)(PMID:21929696)
  6. Manceau V et al. The protein kinase KIS impacts gene expression during development and fear conditioning in adult mice. PLoS One 7, e43946 (2012)(PMID:22937132)
  7. Kajiwara T et al. SAP155-mediated c-myc suppressor far-upstream element-binding protein-interacting repressor splicing variants are activated in colon cancer tissues. Cancer Sci.104, 149-56 (2013)(PMID:23113893)
  8. Gao Y et al. Regulation of HPV16 E6 and MCL1 by SF3B1 inhibitor in head and neck cancer cells. Sci Rep. 4, 6098 (2014)(PMID:25139387)
  9. Kfir N et al. SF3B1 association with chromatin determines splicing outcomes. Cell Rep. 11, 618-29 (2015)(PMID:25892229)
  10. Schreiber CA et al. An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses. PLoS Pathog. 11, e1005082 (2015)(PMID:26244496)
  11. Lee SC et al. Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins. Nat Med. 22, 672-8 (2016)(PMID:27135740)
  12. Cossa G et al. Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription. Mol Cell. 77, 1322-1339.e11 (2019)(PMID:32006464)

Immunoprecipitation

  1. Kotake Y et al. Splicing factor SF3b as a target of the antitumor natural product pladienolide. Nat Chem Biol. 3, 570-5 (2007)(PMID:17643112)
  2. Tanuma N et al. Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1 (PP1) to dephosphorylate the U2 small nuclear ribonucleoprotein particle (snRNP) component, spliceosome-associated protein 155 (Sap155). J Biol Chem. 283, 35805-14 (2008)(PMID:18842582)
  3. Kfir N et al. SF3B1 association with chromatin determines splicing outcomes. Cell Rep. 11, 618-29 (2015)(PMID:25892229)

Immunohistochemistry

  1. Sciarrillo R et al. Splicing modulation as novel therapeutic strategy against diffuse malignant peritoneal mesothelioma. EBioMedicine 39, 215-225 (2019)(PMID:30581150)
Product category
Research area
RNA-RNP Network
Epigenetics
Cancer
この製品に関するお問い合わせをする

  • The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.