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HOME > Product search results > Code No. D115-3 Anti-CENP-A (Human) mAb

Code No.　D115-3

Anti-CENP-A (Human) mAb

Price

¥44,000

Availability (in Japan)

10 or more

（In Japan at 17:00,
Apr 20, 2024 in JST）

Size

100 µL (1 mg/mL)

Data

  • Western Blotting

  • Immunocytochemistry

  • Immunohistochemistry
Clonality Monoclonal Clone 3-19
Isotype (Immunized Animal) Mouse IgG1
Applications
WB
1 µg/mL  
IC
10 µg/mL  
IH
5 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
ChIP*
reported.  （PMID: 11884609 / 15009096）
Immunogen (Antigen) Human CENP-A peptide (3-19 aa)
Reactivity [Gene ID]

Human[1058], Mouse(-), Rat(-)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names CenH3, CENPA
Background Centromere protein A (CENP-A) is a　17-19 kDa centromere-specific histone variant that is 62%　identical to the carboxy-terminal domain of histone H3. In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A, and H2B. CENP-A defines active centromere regions by forming centromere-specific nucleosomes on which kinetochores are assembled. CENP-A is specifically located to the inner plate of the kinetochore and it is essential for kinetochore targeting of CENP-C and other kinetochore components. CENP-A is required for nucleosomal packaging of centromeric DNA at interphase.
Related products M146-3 Anti-Nuf2 (Human) mAb
Citations

Western Blotting

  1. Obuse C et al. Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase. Genes Cells. 9, 105-20 (2004)(PMID:15009096)
  2. Slattery SD et al. Aurora-C and Aurora-B share phosphorylation and regulation of CENP-A and Borealin during mitosis. Cell Cycle. 7, 787-95 (2008)(PMID:18239465)
  3. Okada M et al. CENP-H-containing complex facilitates centromere deposition of CENP-A in cooperation with FACT and CHD1. Mol Biol Cell. 20, 3986-95 (2009)(PMID:19625449)
  4. Ando S et al. CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells. Mol Cell Biol. 22, 2229-41 (2002)(PMID:11884609)
  5. Slattery SD et al. Aurora-C and Aurora-B share phosphorylation and regulation of CENP-A and Borealin during mitosis. Cell Cycle 7, 787-95 (2008)(PMID:18239465)
  6. Kazami T et al. Nuclear accumulation of annexin A2 contributes to chromosomal instability by coilin-mediated centromere damage. Oncogene 34, 4177-89 (2015)(PMID:25347736)

Immunoprecipitation

  1. Ando S et al. CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells. Mol Cell Biol. 22, 2229-41 (2002)(PMID:11884609)

Immunocytochemistry

  1. Mimori-Kiyosue Y et al. Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment. Genes Cells. 11, 845-57 (2006)(PMID:16866869)
  2. Izuta H et al. Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells. Genes Cells. 11, 673-84 (2006)(PMID:16716197)
  3. Obuse C et al. Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase. Genes Cells. 9, 105-20 (2004)(PMID:15009096)
  4. Marshall OJ et al. Three-dimensional localization of CENP-A suggests a complex higher order structure of centromeric chromatin. J Cell Biol. 183, 1193-202 (2008)(PMID:19114591)
  5. Okada M et al. CENP-H-containing complex facilitates centromere deposition of CENP-A in cooperation with FACT and CHD1. Mol Biol Cell. 20, 3986-95 (2009)(PMID:19625449)
  6. Chi YH et al. Histone acetyltransferase hALP and nuclear membrane protein hsSUN1 function in de-condensation of mitotic chromosomes. J Biol Chem. 282, 27447-58 (2007)(PMID:17631499)
  7. Orthaus S et al. RNAi knockdown of human kinetochore protein CENP-H. Biochem Biophys Res Commun 348, 36-46 (2006)(PMID:16875666)
  8. Ando S et al. CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells. Mol Cell Biol. 22, 2229-41 (2002)(PMID:11884609)
  9. Hemmerich P et al. Dynamics of inner kinetochore assembly and maintenance in living cells. J Cell Biol. 180, 1101-14 (2008)(PMID:18347072)
  10. Okada M et al. CENP-H-containing complex facilitates centromere deposition of CENP-A in cooperation with FACT and CHD1. Mol Cell Biol. 20, 3986-95 (2009)(PMID:19625449)
  11. Marshall OJ et al. Three-dimensional localization of CENP-A suggests a complex higher order structure of centromeric chromatin. J Cell Biol. 183, 1193-202 (2008)(PMID:19114591)

Immunohistochemistry

  1. Gobin M et al. A DNA Repair and Cell-Cycle Gene Expression Signature in Primary and Recurrent Glioblastoma: Prognostic Value and Clinical Implications. Cancer Res. 79, 1226-1238 (2019) (PMID:30674534)

Other

  1. Obuse C et al. Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase. Genes Cells. 9, 105-20 (2004)(PMID:15009096)
  2. Ando S et al. CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells. Mol Cell Biol. 22, 2229-41 (2002)(PMID:11884609)
Product category
Research area
Epigenetics
Cancer
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  • The availability is based on the information in Japan at 17:00, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.