抗体 製品詳細情報

Code No. RN014MW
Anti-SFPQ (PSF) mAb
価格(税別) ¥52,800
包装 100 µL (1 mg/mL)
在庫 10以上
(2024/04/18 17:00時点)
クローナリティー Monoclonal
クローン C23
アイソタイプ
(免疫動物)
Mouse IgG2aκ
使用法 WB 1 µg/mL  
IP 2 µg/sample  
IC 0.5 µg/mL  
IH 1 µg/mL  
免疫原(抗原) KLH-conjugated synthetic peptide, CAGYGRGREEYEGPNKKPRF (corresponding to amino acid residues 681-699 of mouse Sfpq)
交差性 [Gene ID]

Human[6421], Mouse[71514], Rat[252855], Hamster[100761355]

性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 POMP100, PPP1R140
背景 Paraspeckle is one of newly identified subnuclear bodies consists of RNAs and RNA-binding proteins. SFPQ, also known as PSF, is a core component of paraspeckle and belongs to DBHS protein family. SFPQ forms heteromer by binding to other DHBS protein family, such as p54/NONO, and participates in mRNA splicing and transcriptional regulation in the nucleus. In Alzheimer's and Pick's diseases known as tauopathy, SFPQ is accumulated in cytoplasm in neurons and astrocytes. Altered localization of SFPQ together with tau mutation may be involved in pathogenesis of neurodegenerative diseases.
関連製品 RN013MW Anti-Nono (P54NRB) mAb
RN092PW Anti-NONO (P54NRB) pAb
M019-3 Anti-Nucleolin mAb
M041-3 Anti-PML (Human) mAb
PM064 Anti-Lamin B1 pAb
PM091 Anti-Per1 (Mouse) pAb
PM093 Anti-NR1D2 (Rev-erbβ) pAb
製品カテゴリー 分野:RNA-RNPネットワーク エピジェネティクス
ブランド:RiboCluster Profiler™
データ





使用文献

Western Blotting

  1. Yamazaki T et al. Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation. Mol Cell. 70, 1038-1053.e7. (2018)(PMID:29932899)

Immunocytochemistry

  1. Yamazaki T et al. Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation. Mol Cell. 70, 1038-1053.e7. (2018)(PMID:29932899)
参考文献
  1. Imamura K et al. Long noncoding RNA NEAT1-dependent SFPQ relocation from promoter region to paraspeckle mediates IL8 expression upon immune stimuli. Mol Cell 53, 393-406 (2014) (PMID:24507715)
  2. Hirose T et al. NEAT1 long noncoding RNA regulates transcription via protein sequestration within subnuclear bodies. Mol Biol Cell 25,169-83 (2014) (PMID:24173718)
  3. Elzbieta K et al. Distinct roles of DBHS family members in the circadian transcriptional feedback loop. Mol Cell Biol. 32, 4585-94 (2012) (PMID:22966205)
  4. Nakagawa S, Hirose T. Paraspeckle nuclear bodies--useful uselessness? Cell Mol Life Sci. 69, 3027–36 (2012) (PMID:22476590)
  5. Nakagawa S et al. Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice. J Cell Biol. 193, 31-9 (2011) (PMID:21444682)
  6. Kuwahara S et al. PSPC1, NONO, and SFPQ are expressed in mouse Sertoli cells and may function as coregulators of androgen receptor-mediated transcription. Biol Reprod. 75, 352-9 (2006) (PMID:16641145)
  7. Fox AH et al. Paraspeckles: a novel nuclear domain. Curr Biol. 12, 13-25 (2002) (PMID:11790299)

※在庫につきましては2024年04月18日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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