抗体 製品詳細情報

Code No. RN011P プリントプリント
Anti-PTBP1 (Human) pAb
価格(税別) ¥60,000
包装 200 µg/200 µL
在庫 10以上
(2019/09/17 17:00時点)
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit Ig (aff.)
使用法 WB 1:1,000  
IP 5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC* reported.  (PMID: 24865424 / 26990993)
RIP 15 µg/500 µL of cell extract from 6 x 106 cells  
Other(eCLIP) reported.  (PMID: 27018577)
免疫原(抗原) KLH-conjugated synthetic peptide KFKGDSRSAGVPSR (46-59 a. a.)
交差性 [Gene ID]

Human[5725], Mouse(-), Rat(-), Hamster(-)

性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 polypyrimidine tract binding protein 1, HNRNP-I, HNRNPI, HNRPI
背景 The polypyrimidine tract binding protein 1 (PTBP1), a 57 kDa RNA binding protein also known as hnRNP1, has been implicated in multiple aspect of mRNA metabolism, including regulation of alternative splicing, internal ribosome entry site-driven translation, mRNA localization, and polyadenylation. Four of tandem RNA recognition motif domains give PTBP1 a strong binding activity to RNA. Posttranscriptional regulation of gene expression by PTBP1 has been reported on many genes, including CD154, insulin, VEGF and Human inducible nitric oxide synthase.
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RN1005 RIP-Assay Kit for microRNA
RN052PW Anti-HNRNPC pAb
RN060PW Anti-HNRNPD (AUF1) pAb
RN019P Anti-HNRNPK pAb
RN024P Anti-PCBP1 pAb
RN025P Anti-PCBP2 pAb
RN011P Anti-PTBP1 (Human) pAb
RN114PW Anti-HNRNPA1 pAb
製品カテゴリー 分野:RNA-RNPネットワーク
ブランド:使用文献・データ募集プレゼント対象製品 RiboCluster Profiler™
データ



使用文献

Western Blotting

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
  2. Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893)
  3. Cui J, Placzek WJ. PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell Death Differ. 23, 1681-90 (2016)(PMID:27367564)
  4. Nussbacher JK, Yeo GW. Systematic Discovery of RNA Binding Proteins that Regulate MicroRNA Levels. Mol Cell. 69, 1005-1016.e7 (2018)(PMID:29547715)
  5. Cui J, Placzek WJ. PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 and promotes miR-101-induced apoptosis. Cell Death Dis. 9, 552 (2018)(PMID:29748555)

Immunoprecipitation

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)

Immunocytochemistry

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
  2. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)

RNP Immunoprecipitation

  1. Ferrarese R et al. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression. J Clin Invest. 124, 2861-74 (2014)(PMID:24865424)
  2. Cui J, Placzek WJ. PTBP1 modulation of MCL1 expression regulates cellular apoptosis induced by antitubulin chemotherapeutics. Cell Death Differ. 23, 1681-90 (2016)(PMID:27367564)
  3. Wang ZN et al. High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin. Oncotarget 8, 36185-36202 (2017)(PMID:28404950)

Other(eCLIP)

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)

※在庫につきましては2019年09月17日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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