抗体 製品詳細情報

Code No. RN009P
Anti-IGF2BP3 (IMP3) pAb
価格(税別) ¥66,000
包装 200 µL (1 mg/mL)
在庫 10以上
（2024/04/18 17:00時点）
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)		 Rabbit Ig (aff.)
使用法 WB　1:1,000  
IP　5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC*　（PMID: 26990993 / 27068461）
RIP　15 µg/500 µL of cell extract from 1.2 x 107 cells  
Other(eCLIP)　reported.  （PMID: 27068461 / 27108577）
Other(iCLIP)　reported.  （PMID: 26974154）
免疫原(抗原) KLH-conjugated synthetic peptide HQQQKALQSGPPQSRRK (563-579 aa)
交差性 [Gene ID]

Human[10643], Mouse[140488], Rat(-), Hamster(-)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 IGF2BP3, insulin-like growth factor 2 mRNA binding protein 3, CT98, KOC1, IMP-3, VICKZ3/IMP3, Koc13, mimp3, Neilsen
背景 IGF2BP3/IMP3 is identical to the KH domain containing protein overexpressed in cancer (KOC). The protein encoded by this gene was found to bind in vivo to IGF2 leader 3 and leader 4 mRNAs and H19 RNA. Binding of IMP3 to the 5' UTR of the IGF2 leader 3 mRNA represses translation of IGF2 during late development. The encoded protein contains several KH domains, which are important for RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. The growth regulating gene IMP3 has been characterized as a candidate for Silver-Russell syndrome.
関連製品 RN1001 RIP-Assay Kit
RN008P Anti-IGF2BP2 (IMP2) pAb
RN007P Anti-IGF2BP1 (IMP1) pAb
RN001M Anti-IGF2BP1 (IMP1) mAb
RN1005 RIP-Assay Kit for microRNA
製品カテゴリー 分野:RNA-RNPネットワーク がん
ブランド:RiboCluster Profiler™
データ



使用文献

Western Blotting

  1. Ueki A et al. Up-regulation of Imp3 confers in vivo tumorigenicity on murine osteosarcoma cells. PLoS One. 7, e50621 (2012)(PMID:23226335)
  2. Lederer M et al. The role of the oncofetal IGF2 mRNA-binding protein 3 (IGF2BP3) in cancer. Semin Cancer Biol. 29, 3-12 (2014)(PMID:25068994)
  3. Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA ｔargets in ｈuman pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  4. Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep 15, 1634-47 (2016)(PMID:27184842)
  5. JnBaptiste CK et al. Dicer loss and recovery induce an oncogenic switch driven by transcriptional activation of the oncofetal Imp1-3 family. Genes Dev. 31, 674-687 (2017)(PMID:28446596)
  6. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell. 171, 1559-1572 (2017)(PMID:29373831)

Immunoprecipitation

  1. Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA ｔargets in ｈuman pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  2. Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell. 171, 1559-1572 (2017)(PMID:29373831)

Immunocytochemistry

  1. Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
  2. Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA ｔargets in ｈuman pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  3. Markmiller S et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell. 172, 590-604.e13 (2018)(PMID:29373831)

RNP Immunoprecipitation

  1. Pasiliao CC et al. The involvement of insulin-like growth factor 2 binding protein 3 (IMP3) in pancreatic cancer cell migration, invasion, and adhesion. BMC Cancer 15, 266 (2015)(PMID:25886367)
  2. Ennajdaoui H et al. IGF2BP3 modulates the Interaction of invasion-associated transcripts with RISC. Cell Rep. 15, 1876-83 (2016)(PMID:27210763)
  3. Kim HY et al. IMP2 and IMP3 cooperate to promote the metastasis of triple-negative breast cancer through destabilization of progesterone receptor. Cancer Lett. 415, 30-39 (2018)(PMID:29217458)

Other(eCLIP)

  1. Conway AE et al. Enhanced CLIP uncovers IMP protein-RNA ｔargets in ｈuman pluripotent stem cells important for cell adhesion and survival. Cell Rep. 15, 666-79 (2016)(PMID:27068461)
  2. Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27108577)

Other(iCLIP)

  1. Palanichamy JK et al. RNA-binding protein IGF2BP3 targeting of oncogenic transcripts promotes hematopoietic progenitor proliferation. J Clin Invest. 126, 1495-511 (2016)(PMID:26974154)

※在庫につきましては2024年04月18日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。

MBLCopyright (C)2015 Medical ＆ Biological Laboratories Co., Ltd. All Rights Reserve