抗体 製品詳細情報

Code No. RN004P プリントプリント
Anti-ELAVL1 (HuR) pAb
価格(税別) ¥60,000
包装 200 µg/200 µL
在庫 10以上
(2019/09/16 17:00時点)
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit Ig (aff.)
使用法 WB 1:1,000  
IP 5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC Can be used.  
RIP 15 µg/500 µL of cell extract from 1.5 x 107 cells  
免疫原(抗原) KLH-conjugated synthetic peptide MSNGYEDHMAEDCRGDIGR (1-19 aa)
交差性 [Gene ID]

Human[1994](23176121), Mouse[15568], Rat[363854], Hamster[100754131]

性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R), HUR, Hua, MelG, ELAV1/HUR, W91709, 2410055N02Rik
背景 ELAVL1/HuR, like other ELAVL proteins, has a short N-terminal region followed by 2 RNA-binding motifs, a basic linker domain, and a third RNA-binding motif. The basic hinge region bears a novel nuclear shuttling sequence which is implicated in the nucleocytoplasmic HuR shuttling. Unlike its neuron-specific ELAV relatives (HuB, HuC and HuD), HuR itself is ubiquitously expressed. Structure-function analyses of HuR have shown that it binds to AU-rich mRNA sequences in the nucleus and may be involved in their nuclear export.
関連製品 RN1001 RIP-Assay Kit
RN1005 RIP-Assay Kit for microRNA
RN1011 RiboTrap Kit
RN005P Anti-ELAVL2 (HuB) (Human) pAb
RN006P Anti-ELAVL3 (HuC) pAb
RN007M Anti-ELAVL1 (HuR) mAb
製品カテゴリー 分野:RNA-RNPネットワーク がん
ブランド:使用文献・データ募集プレゼント対象製品 RiboCluster Profiler™
データ



使用文献

Western Blotting

  1. Onomoto K et al. Critical role of an antiviral stress granule containing RIG-I and PKR in viral detection and innate immunity. PLoS One. 7, e43031 (2012)(PMID:22912779)

RNP Immunoprecipitation

  1. Williams TK et al. pp32 (ANP32A) expression inhibits pancreatic cancer cell growth and induces gemcitabine resistance by disrupting HuR binding to mRNAs. PLoS One. 5, e15455 (2010)(PMID:21152064)
  2. Pineda DM et al. HuR's post-transcriptional regulation of Death Receptor 5 in pancreatic cancer cells. Cancer Biol Ther. 13, 946-55 (2012)(PMID:22785201)
  3. Banadakoppa M et al. Role of transcription factor Sp1 and RNA binding protein HuR in the downregulation of Dr(+) Escherichia coli receptor protein decay accelerating factor (DAF or CD55) by nitric oxide. FEBS J. 280, 840-54 (2013)(PMID:23176121)
  4. Jinbo M et al. Targeting the mRNA-binding protein HuR impairs malignant characteristics of pancreatic ductal adenocarcinoma cells. Oncotarget 6, 27312-31 (2015)(PMID:26314962)
  5. Suhl JA et al. A 3' untranslated region variant in FMR1 eliminates neuronal activity-dependent translation of FMRP by disrupting binding of the RNA-binding protein HuR. PNAS 112, E6553-61 (2015)(PMID:26554012)
  6. Huang YH et al. Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy. Oncotarget 7, 21812-24 (2016)(PMID:26943573)
  7. Romeo C et al. HuR contributes to TRAIL resistance by restricting Death Receptor 4 expression in pancreatic cancer cells. Mol Cancer Res. 14, 599-611 (2016)(PMID:27053682)
  8. Popovitchenko T et al. The RNA binding protein HuR determines the differential translation of autism-associated FoxP subfamily members in the developing neocortex. Sci. Rep. 6, 28998 (2016)(PMID:27383233)
  9. Zhou H et al. GPRC5A is a potential oncogene in pancreatic ductal adenocarcinoma cells that is upregulated by gemcitabine with help from HuR. Cell Death Dis. 7, e2294 (2016)(PMID:27415424)

※在庫につきましては2019年09月16日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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