抗体 製品詳細情報

Code No. RN003M
Anti-EIF2C2 (AGO2) (Human) mAb
価格(税別) ¥66,000
包装 200 µL (1 mg/mL)
在庫 10以上
（2024/04/24 00:05時点）
クローナリティー Monoclonal
クローン 1B1-E2H5
アイソタイプ
(免疫動物)		 Mouse IgG2aλ
使用法 WB　1-4 µg/mL  
IP　1.5 µg/500 µL of cell extract from 1.5 x 106 cells  
RIP　15 µg/500 µL of cell extract from 1.5 x 107 cells  
免疫原(抗原) Recombinant partial EIF2C2 (N-terminal region, approximately 180 a.a.)
交差性 [Gene ID]

Human[27161], Mouse(-), Rat(-)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 eukaryotic translation initiation factor 2C2, Q10
背景 Eukaryotic translation initiation factor 2C, subunit 2 (EIF2C2; AGO2), is a member of the argonaute protein family. It is a core component of RNA-induced silencing complex (RISC). The EIF2C2 belonging to the human argonaute protein family possesses endonuclease activity and functions as a slicer in gene silencing pathways. It recognizes and cleaves target mRNA by RNA interference. In small interference RNA (siRNA) pathway, EIF2C2 forms a complex with EIF2C1 and siRNA, and in microRNA (miRNA) pathway, it binds EIF2C3 to miRNA. EIF2C2 expression is controlled by epidermal growth factor receptor and mitogen-activated protein kinase signaling in human breast cancer cell line.
関連製品 RN1001 RIP-Assay Kit
RN1005 RIP-Assay Kit for microRNA
RN030PW Anti-DICER1 (Human) pAb
RN058PW Anti-TARBP2 pAb
RN063PW Anti-DHX9 pAb
RN033P Anti-TNRC6A (GW182) (Human) pAb
PD009 Anti-rck (p54) pAb
PM044 Anti-PIWIL2 (MILI) (Mouse) pAb
D365-3 Anti-PIWIL2 (MILI) (Mouse) mAb
製品カテゴリー 分野:RNA-RNPネットワーク
ブランド:RiboCluster Profiler™
データ



使用文献

Western Blotting

  1. Fukao A et al. MicroRNAs Trigger Dissociation of eIF4AI and eIF4AII from Target mRNAs in Humans. Mol Cell 56, 79-89 (2014)(PMID:25280105)
  2. Degrauwe N et al. The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing. Cell Rep. 15, 1634-47 (2016)(PMID:27184842)
  3. Yamane D et al. Differential hepatitis C virus RNA target site selection and host factor activities of naturally occurring miR-122 3' variants. Nucleic Acids Res. 45, 4743-4755 (2017)(PMID:28082397)
  4. Cui J, Placzek WJ. PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 and promotes miR-101-induced apoptosis. Cell Death Dis. 9, 552 (2018)(PMID:29748555)

RNP Immunoprecipitation

  1. Zhang LY et al. MicroRNA-144 promotes cell proliferation, migration and invasion in nasopharyngeal carcinoma through repression of PTEN. Carcinogenesis 34, 454-63 (2013)(PMID:23125220)
  2. Comincini S et al. microRNA-17 regulates the expression of ATG7 and modulates the autophagy process, improving the sensitivity to temozolomide and low-dose ionizing radiation treatments in human glioblastoma cells. Cancer Biol Ther. 14, 574-86 (2013)(PMID:23792642)
  3. Marchesi N et al. Autophagy is modulated in human neuroblastoma cells through direct exposition to low frequency electromagnetic fields. J Cell Physiol. 229, 1776-86 (2014)(PMID:24676932)
  4. Selitsky SR et al. Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C. Sci Rep. 5, 7675 (2015)(PMID:25567797)
  5. Lopez P et al. RNA Activation of the Vascular Endothelial Growth Factor Gene (VEGF) Promoter by Double-Stranded RNA and Hypoxia: Role of Noncoding VEGF Promoter Transcripts. Mol Cell Biol. 36, 1480-93 (2016)(PMID:26976645)
  6. Yamane D et al. Differential hepatitis C virus RNA target site selection and host factor activities of naturally occurring miR-122 3' variants. Nucleic Acids Res. 45, 4743-4755 (2017)(PMID:28082397)
  7. Al-Haidari AA et al. MiR-155-5p positively regulates CCL17-induced colon cancer cell migration by targeting RhoA. Oncotarget. 8, 14887-14896 (2017)(PMID:28146427)
  8. Cui J, Placzek WJ. PTBP1 enhances miR-101-guided AGO2 targeting to MCL1 and promotes miR-101-induced apoptosis. Cell Death Dis. 9, 552 (2018)(PMID:29748555)
  9. Bach DH et al. BMP4 Upregulation Is Associated with Acquired Drug Resistance and Fatty Acid Metabolism in EGFR-Mutant Non-Small-Cell Lung Cancer Cells. Mol Ther Nucleic Acids. 12, 817-828 (2018)(PMID:30153566)
  10. Wen J et al. miR-424 coordinates multilayered regulation of cell cycle progression to promote esophageal squamous cell carcinoma cell proliferation. EBioMedicine. 37, 110-124 (2018) (PMID:30361064)

※在庫につきましては2024年04月24日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。

MBLCopyright (C)2015 Medical ＆ Biological Laboratories Co., Ltd. All Rights Reserve