抗体 製品詳細情報

Code No. PM044
Anti-PIWIL2 (MILI) (Mouse) pAb
価格(税別) ¥52,800
包装 100 µL
在庫 10以上
（2024/04/25 17:00時点）
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)		 Rabbit Ig (aff.)
使用法 WB　1:1,000  
IP　1 µL/sample  
IH*　reported.  （PMID: 29018194）
RIP*　reported.  （PMID: 18381894 / 19735482 / 20439430）
免疫原(抗原) synthetic peptide VRKDREEPRSSLPDPS corresponding to Mouse PIWIL2 (107-122 a.a.)
交差性 [Gene ID]

Mouse[57746]
性状 PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
背景 The Piwi/Argonaute protein family plays important roles in stem-cell self-renewal, RNA silencing and translational regulation in various organisms. The family members are defined by conserved both PAZ (Piwi Argonaut and Zwille) and Piwi domains participate in RNA binding. Piwi-interacting RNA (piRNA) is a class of small RNA molecules that is expressed uniquely in mammalian testes and forms RNA-protein complexes with Piwi proteins. These small regulatory RNAs have been implicated in spermatogenesis, repression of retrotransposon transposition in germline cells, epigenetic regulation and positive regulation of translation and mRNA stability. MIWI (PIWIL1) and MILI (PIWIL2) are mouse homologues of Piwi. MILI is expressed from primordial germ cells (PGC) in embryo, whereas MIWI is detected later from the mid-pachytene stage to early round spermatids. Their expression overlaps in the mid-pachytene and early round spermatid stage. The expression patterns and knockout phenotypes of mammalian Piwi members indicated MILI is an essential factor for meiotic differentiation during spermatogenesis.
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製品カテゴリー 分野:幹細胞研究 RNA-RNPネットワーク エピジェネティクス がん
データ

使用文献

Western Blotting

  1. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  2. Kuramochi-Miyagawa S et al. MVH in piRNA processing and gene silencing of retrotransposons. Genes Dev. 24, 887-92 (2010)(PMID:20439430)
  3. Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194)
  4. Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670)
  5. Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019) (PMID:30590800)

Immunoprecipitation

  1. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  2. Shiromoto Y et al. GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells. RNA 19, 803-10 (2013)(PMID:23611983)

Immunohistochemistry

  1. Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194)
  2. Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670)
  3. Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019)(PMID:30590800)

RNP Immunoprecipitation

  1. Kuramochi-Miyagawa S et al. DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. Genes Dev. 22, 908-17 (2008)(PMID:18381894)
  2. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  3. Kuramochi-Miyagawa S et al. MVH in piRNA processing and gene silencing of retrotransposons. Genes Dev. 24, 887-92 (2010)(PMID:20439430)
  4. Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194)
  5. Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670)
  6. Nishimura T et al. PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep. 19, e44957 (2018)(PMID:29444933)
  7. Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019)(PMID:30590800)

※在庫につきましては2024年04月25日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
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※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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