抗体 製品詳細情報

Code No. M152-3MS
Anti-LC3 mAb for My select sampler set
価格(税別) ¥13,200
包装 20 µL (2 mg/mL)
在庫 1-9
（2024/04/20 17:00時点）
クローナリティー Monoclonal
クローン 4E12
アイソタイプ
(免疫動物)		 Mouse IgG1 κ
使用法 WB　5 µg/mL  
IP　5 µg/300 µL of cell extract from 1x107 cells  
FCM　40 µg/mL (final concentration)  
IC　40 µg/mL  
IH*　reported.  （PMID: 21957238 / 19955365 / 19915056）
Other(Immuno-EM)　20 µg/mL  
免疫原(抗原) Recombinant Human LC3 (MAP1LC3B: 1-120 a.a.)
交差性 [Gene ID]

Human[81631/84557], Mouse[67443/66734], Rat[64862/362245], Hamster[100760576/100769810]
性状 2 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 microtubule-associated protein 1 light chain 3 beta, microtubule-associated protein 1 light chain 3 alpha, LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, MAP1LC3A
背景 Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I. A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II. This antibody can detect both forms of LC3.
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PM036-PN Positive control for anti-LC3 antibody
M133-3 Anti-Atg3 mAb
PM036 Anti-LC3 pAb
M186-3 Anti-LC3 mAb
PD014 Anti-LC3 pAb
PM072 Anti-VMP1 pAb
PM045 Anti-p62 (SQSTM1) pAb
M162-3 Anti-p62 (SQSTM1) (Human) mAb
PM066 Anti-p62 C-terminal pAb
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PM037 Anti-GABARAP pAb
8486 Autophagy Watch
備考 FAQ：よくあるお問い合わせを見る→
本製品はMy select sampler set対象品です。詳細はこちらをご覧ください。
製品カテゴリー 分野:オートファジー
データ


使用文献

Western Blotting

  1. Ho H et al. WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition. J Biol Chem. 286, 12509-23 (2011)(PMID:21317285)
  2. Saiki S et al. Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition. Autophagy 7, 176-187 (2011)(PMID:21081844)
  3. Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
  4. Wang Y et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 283,　4766-77 (2008)(PMID:18073215)
  5. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)

Immunoprecipitation

  1. Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)(PMID:19955365)

Immunocytochemistry

  1. Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
  2. Nishitsuji K et al. The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Am J Pathol. 174, 957-69 (2009)(PMID:19164507)
  3. Wang Y et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 283,　4766-77 (2008)(PMID:18073215)
  4. Chalazonitis A et al. Homeodomain interacting protein kinase 2 regulates postnatal development of enteric dopaminergic neurons and glia via BMP signaling. J Neurosci. 31, 13746-57 (2011)(PMID:21957238)
  5. Mori H et al. Induction of selective autophagy in cells replicating hepatitis C virus genome. J Gen Virol. 99, 1643-1657 (2018)(PMID:30311874)

Immunohistochemistry

  1. Kasai M et al. Autophagic compartments gain access to the MHC class II compartments in thymic epithelium. J Immunol. 183, 7278-85 (2009)(PMID:19915056)
  2. Mookerjee S et al. Posttranslational modification of ataxin-7 at lysine 257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment. J Neurosci. 29, 15134-44 (2009)(PMID:19955365)

Other(Immuno-EM)

  1. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)

※在庫につきましては2024年04月20日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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