抗体 製品詳細情報

Code No. K0205-3
Anti-Txnip (VDUP1) mAb
価格(税別) ¥33,000
包装 100 µL (1 mg/mL)
在庫 10以上
（2024/04/18 00:05時点）
クローナリティー Monoclonal
クローン JY2
アイソタイプ
(免疫動物)		 Mouse IgG1
使用法 WB　1 µg/mL  
IP　2 µg/200 µL of cell extract from 5x106 cells  
IC*　reported.  （PMID: 27929088）
IH*　reported.  （PMID: 19959470 / 23584857）
免疫原(抗原) Recombinant Human Txnip/VDUP1
交差性 [Gene ID]

Human[10628], Mouse[56338], Rat*[117514](PMID: 19258488/23803610/26858253)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 thioredoxin interacting protein, THIF, VDUP1
背景 Thioredoxin (TRX)-binding protein (Txnip), also called Vitamin D3 up-regulated protein 1 (VDUP1) is an endogenous inhibitor of TRX. Redox-dependent regulation of Txnip by mitogenic factors through Reactive oxygen species (ROS) and the specific binding of Txnip to the redox-sensitive cysteine-sulfide center of TRX modulate intracellular levels of ROS and the mitogenic activity of TRX. It has been reported that Txnip plays important roles in diverse cellular processes, including the regulation of cellular redox balance, apoptosis, proliferation, and differentiation.
関連製品 K0204-3 Anti-Txnip (VDUP1) mAb
M013-3 Anti-Thioredoxin (Trx-tag) mAb
M075-3 Mouse IgG1 (isotype control)
製品カテゴリー 分野:細胞ストレス
データ


使用文献
  1. Pang ST et al. Thioredoxin-interacting protein: an oxidative stress-related gene is upregulated by glucose in human prostate carcinoma cells. J Mol Endocrinol. 42, 205-14 (2009)(PMID:19052253)

Western Blotting

  1. Yoshioka J et al. Targeted deletion of thioredoxin-interacting protein regulates cardiac dysfunction in response to pressure overload. Circ Res. 101, 1328-38 (2007)(PMID:17916779)
  2. Chen J et al. Thioredoxin-interacting protein: a critical link between glucose toxicity and beta-cell apoptosis. Diabetes 57, 938-44 (2008)(PMID:18171713)
  3. Chen J et al. Diabetes induces and calcium channel blockers prevent cardiac expression of proapoptotic thioredoxin-interacting protein. Am J Physiol Endocrinol Metab. 296, E1133-9 (2009)(PMID:19258488)
  4. Patwari P et al. Thioredoxin-independent regulation of metabolism by the alpha-arrestin proteins. J Biol Chem. 284, 24996-5003 (2009)(PMID:19605364)
  5. Saxena G et al. Intracellular shuttling and mitochondrial function of thioredoxin-interacting protein. J Biol Chem. 285, 3997-4005 (2010)(PMID:19959470)
  6. Kibbe C et al. FOXO1 competes with carbohydrate response element-binding protein (ChREBP) and inhibits thioredoxin-interacting protein (TXNIP) transcription in pancreatic beta cells. J Biol Chem. 288, 23194-202 (2013)(PMID:23803610)
  7. Jing G et al. Thioredoxin-interacting protein promotes islet amyloid polypeptide expression through miR-124a and FoxA2. J Biol Chem. 289, 11807-15 (2014)(PMID:24627476)
  8. Hong K et al. Cytokines Regulate β-Cell Thioredoxin-interacting Protein (TXNIP) via Distinct Mechanisms and Pathways. J Biol Chem. 291, 8428-39 (2016)(PMID:26858253)
  9. Ahn B et al. MondoA coordinately regulates skeletal myocyte lipid homeostasis and insulin signaling. J Cin Invest. 126, 3567-79 (2016)(PMID:27500491)
  10. Hulmi JJ et al. Effects of muscular dystrophy, exercise and blocking activin receptor IIB ligands on the unfolded protein response and oxidative stress. Free Radic  Biol Med. 99, 308-322 (2016)(PMID:27554968)
  11. Du RH et al. Uncoupling protein 2 modulation of the NLRP3 inflammasome in astrocytes and its implications in depression. Redox Biol. 9, 178-187 (2016)(PMID:27566281)
  12. Coucha M et al. THigh fat diet dysregulates microRNA-17-5p and triggers retinal inflammation: Role of endoplasmic-reticulum-stress. World J Diabetes. 8, 56-65 (2017)(PMID:28265343)
  13. Morita S et al. Targeting ABL-IRE1α Signaling Spares ER-Stressed Pancreatic β Cells to Reverse Autoimmune Diabetes. Cell Metab. 25, 863-897.e8. (2017)(PMID:28380378)
  14. Park S et al. Proteasome inhibitor-induced cleavage of HSP90 is mediated by ROS generation and caspase 10-activation in human leukemic cells. Redox Biol. 13, 470-476 (2017)(PMID:28715732)
  15. Hoshikawa H et al. Combined treatment with D-allose, docetaxel and radiation inhibits the tumor growth in an in vivo model of head and neck cancer. Oncol Lett. 15, 3422-3428 (2018)(PMID:29456721)
  16. Belaya I et al. Long-Term Exercise Protects against Cellular Stresses in Aged Mice. Oxid Med Cell Longev. 2018, 2894247 (2018)(PMID:29765493)
  17. Panse M　et al. Palmitate and insulin counteract glucose-induced thioredoxin interacting protein (TXNIP) expression in insulin secreting cells via distinct mechanisms. PLoS One. 13, e0198016 (2018)(PMID:29813102)
  18. Gunes A et al. Thioredoxin interacting protein promotes invasion in hepatocellular carcinoma. Oncotarget. 9, 36849-36866 (2018) (PMID:30627326)
  19. Danzl K et al. Early inhibition of endothelial retinoid uptake upon myocardial infarction restores cardiac function and prevents cell, tissue, and animal death. J Mol Cell Cardiol. 126 105-117 (2019)(PMID:30472251)
  20. Levring TB et al. Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells. Sci Rep. 9, 16725 (2019) (PMID:31723203)
  21. Ishibashi T et al. Nicotinic acetylcholine receptor signaling regulates inositol-requiring enzyme 1α activation to protect β-cells against terminal unfolded protein response under irremediable endoplasmic reticulum stress. J Diabetes Investig. 11, 801-813 (2020)(PMID:31925927)

Immunocytochemistry

  1. Jung H et al. Thioredoxin-interacting protein regulates haematopoietic stem cell ageing and rejuvenation by inhibiting p38 kinase activity. Nat Commun. 7, 13674 (2016)(PMID:27929088)

Immunohistochemistry

  1. Saxena G et al. Intracellular shuttling and mitochondrial function of thioredoxin-interacting protein. J Biol Chem. 285, 3997-4005 (2010)(PMID:19959470)
  2. Hand LE et al. Induction of the metabolic regulator Txnip in fasting-induced and natural torpor. Endocrinology 154, 2081-91 (2013)(PMID:23584857)
  3. Morrison JA et al. Thioredoxin interacting protein (TXNIP) is a novel tumor suppressor in thyroid cancer. Mol Cancer. 13, 62 (2014)(PMID:24645981)
  4. Danzl K et al. Early inhibition of endothelial retinoid uptake upon myocardial infarction restores cardiac function and prevents cell, tissue, and animal death. J Mol Cell Cardiol. 126 105-117 (2019) (PMID:30472251 )

※在庫につきましては2024年04月18日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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