抗体 製品詳細情報

Code No. K0055-3
Anti-FHL2 mAb
This clone dose not cross-react with FHL1, FHL3, FHL4 and ACT.
価格(税別) ¥33,000
包装 100 µL (1 mg/mL)
在庫 10以上
（2024/04/25 00:05時点）
クローナリティー Monoclonal
クローン 11-134
アイソタイプ
(免疫動物)		 Mouse IgG2a
使用法 WB　1-5 µg/mL  
IP　3 µg/200-300 µL of cell extract  
IC*　reported.  （PMID: 25855776 / 26320172 / 26973248）
IH　1-5 µg/mL (Heat treatment is necessary for paraffin embedded sections.)  
ChIP*　reported.  （PMID: 18378678 / 19265191 / 26025865）
免疫原(抗原) Recombinant Human full length FHL2
交差性 [Gene ID]

Human[2274], Mouse[14200]
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 four and a half LIM domains 2, DRAL, AAG11, FHL-2, SLIM3, SLIM-3
背景 Proteins containing LIM domains (which are double zinc finger motifs implicated in protein binding) are important regulators of cell growth, cell differentiation, and remodeling of the cell cytoskeleton. Human four-and-a-half LIM domains 2 (FHL2), also known as DRAL/Slim3 is a 32 kDa protein expressed predominantly in human heart and to a lesser extent in skeletal muscle, testis, and prostate epithelium. Since FHL2 is abundant in heart tissue, it may play a role in the regulation of myofibrillogenesis of heart via LIM-domain binding to focal adhesions. FHL2 has also been identified as a coactivator of the androgen receptor where it promotes androgen receptor transcriptional activity. Stimulation of the Rho signaling pathway induces translocation of FHL2 to the nucleus and subsequent activation of FHL2- and androgen receptor-dependent genes. FHL2 also acts as a transcriptional repressor in muscle cells　and is involved in modulation of β-catenin-dependent transcription of Wnt-responsive genes.
関連製品 597 Anti-TRAF6 (Mouse) pAb
製品カテゴリー 分野:エピジェネティクス がん
データ

使用文献

Western Blotting

  1. Weinert S et al. M line-deficient titin causes cardiac lethality through impaired maturation of the sarcomere. J Cell Biol. 173, 559-70 (2006)(PMID:16702235)
  2. Kang DE et al. Presenilins mediate phosphatidylinositol 3-kinase/AKT and ERK activation via select signaling receptors. Selectivity of PS2 in platelet-derived growth factor signaling. J Biol Chem. 280, 31537-47 (2005)(PMID:16014629)
  3. Sun J et al. FHL2/SLIM3 decreases cardiomyocyte survival by inhibitory interaction with sphingosine kinase-1. Circ Res. 99, 468-76 (2006)(PMID:16888242)
  4. Wang J et al. Suppression of FHL2 expression induces cell differentiation and inhibits gastric and colon carcinogenesis. Gastroenterology 132, 1066-76 (2007)(PMID:17383428)
  5. Labalette C et al. The LIM-only protein FHL2 regulates cyclin D1 expression and cell proliferation. J Biol Chem. 283, 15201-8 (2008)(PMID:18378678)
  6. Labalette C et al. The LIM-only protein FHL2 mediates ras-induced transformation through cyclin D1 and p53 pathways. PLoS One 3, e3761 (2008)(PMID:19018287)
  7. Ewen EP et al. The Mef2A transcription factor coordinately regulates a costamere gene program in cardiac muscle. J Biol Chem. 286, 29644-53 (2011)(PMID:21724844)
  8. Hojayev B et al. FHL2 binds calcineurin and represses pathological cardiac growth. Mol Cell Biol. 32, 4025-34 (2012)(PMID:22851699)
  9. Li SY et al. Four-and-a-Half LIM Domains Protein 2 Is a Coactivator of Wnt Signaling in Diabetic Kidney Disease. J Am Soc Nephrol. 26, 3072-84 (2015)(PMID:25855776)
  10. Rachmin I et al. FHL2 switches MITF from activator to repressor of Erbin expression during cardiac hypertrophy. Int J Cardiol. 195, 85-94 (2015)(PMID:26025865)
  11. Yan Q et al. KLF8 promotes tumorigenesis, invasion and metastasis of colorectal cancer cells by transcriptional activation of FHL2. Oncotarget 6, 25402-17 (2015)(PMID:26320172)
  12. Jin H et al. Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells. Oncogene 35, 5106-18 (2016)(PMID:26973248)
  13. Wu M et al. FOXK1 interaction with FHL2 promotes proliferation, invasion and metastasis in colorectal cancer. Oncogenesis. 5, e271 (2016)(PMID:27892920)
  14. Verset L et al. ADAM-17/FHL2 colocalisation suggests interaction and role of these proteins in colorectal cancer. Tumour Biol. 39, 1010428317695024 (2017)(PMID:28349819)
  15. Zhou SG et al. FHL2 participates in renal interstitial fibrosis by altering the phenotype of renal tubular epithelial cells via regulating the β-βcatenin pathway. Eur Rev Med Pharmacol Sci. 22, 2734-2741 (2018)(PMID:29771425)

ChIP

  1. Labalette C et al. The LIM-only protein FHL2 regulates cyclin D1 expression and cell proliferation. J Biol Chem. 283, 15201-8 (2008)(PMID:18378678)
  2. Neuman NA et al. The four-and-a-half LIM domain protein 2 regulates vascular smooth muscle phenotype and vascular tone. J Biol Chem. 284, 13202-12 (2009)(PMID:19265191)
  3. Rachmin I et al. FHL2 switches MITF from activator to repressor of Erbin expression during cardiac hypertrophy. Int J Cardiol. 195, 85-94 (2015)(PMID:26025865)

Immunoprecipitation

  1. Sun J et al. FHL2/SLIM3 decreases cardiomyocyte survival by inhibitory interaction with sphingosine kinase-1. Circ Res. 99, 468-76 (2006)(PMID:16888242)
  2. Rachmin I et al. FHL2 switches MITF from activator to repressor of Erbin expression during cardiac hypertrophy. Int J Cardiol. 195, 85-94 (2015)(PMID:26025865)

Immunocytochemistry

  1. Li SY et al. Four-and-a-Half LIM Domains Protein 2 Is a Coactivator of Wnt Signaling in Diabetic Kidney Disease. J Am Soc Nephrol. 26, 3072-84 (2015)(PMID:25855776)
  2. Yan Q et al. KLF8 promotes tumorigenesis, invasion and metastasis of colorectal cancer cells by transcriptional activation of FHL2. Oncotarget 6, 25402-17 (2015)(PMID:26320172)
  3. Jin H et al. Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells. Oncogene 35, 5106-18 (2016)(PMID:26973248)
  4. Wu M et al. FOXK1 interaction with FHL2 promotes proliferation, invasion and metastasis in colorectal cancer. Oncogenesis. 5, e271 (2016)(PMID:27892920)

Immunohistochemistry

  1. Wang J et al. Suppression of FHL2 expression induces cell differentiation and inhibits gastric and colon carcinogenesis. Gastroenterology 132, 1066-76 (2007)(PMID:17383428)
  2. Ewen EP et al. The Mef2A transcription factor coordinately regulates a costamere gene program in cardiac muscle. J Biol Chem. 286, 29644-53 (2011)(PMID:21724844)
  3. Hojayev B et al. FHL2 binds calcineurin and represses pathological cardiac growth. Mol Cell Biol. 32, 4025-34 (2012)(PMID:22851699)
  4. Li SY et al. Four-and-a-Half LIM Domains Protein 2 Is a Coactivator of Wnt Signaling in Diabetic Kidney Disease. J Am Soc Nephrol. 26, 3072-84 (2015)(PMID:25855776)
  5. Yan Q et al. KLF8 promotes tumorigenesis, invasion and metastasis of colorectal cancer cells by transcriptional activation of FHL2. Oncotarget 6, 25402-17 (2015)(PMID:26320172)
  6. Jin H et al. Scaffold protein FHL2 facilitates MDM2-mediated degradation of IER3 to regulate proliferation of cervical cancer cells. Oncogene 35, 5106-18 (2016)(PMID:26973248)
  7. Wu M et al. FOXK1 interaction with FHL2 promotes proliferation, invasion and metastasis in colorectal cancer. Oncogenesis. 5, e271 (2016)(PMID:27892920)
  8. Zhou SG et al. FHL2 participates in renal interstitial fibrosis by altering the phenotype of renal tubular epithelial cells via regulating the β-βcatenin pathway. Eur Rev Med Pharmacol Sci. 22, 2734-2741 (2018)(PMID:29771425)

※在庫につきましては2024年04月25日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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