抗体 製品詳細情報

Code No. D346-3
Anti-5-methylcytidine (m5C) mAb
Anti-5-methylcytidine (m5C) mAb (Clone: FMC-9) reacts with 5-methylcytidine (m5C or m5Cyd), 5-methyl-2’-deoxycytidine (m5dC or m5dCyd) and 5-methylcytosine (5mC, 5-mC or m5Cyt).
価格(税別) ¥66,000
包装 100 µL (1 mg/mL)
在庫 1-9
（2024/04/19 00:05時点）
クローナリティー Monoclonal
クローン FMC-9
アイソタイプ
(免疫動物)		 Mouse IgG2aλ
使用法 IP　Can be used.  
IC　5 µg/mL (for cytoplasmic staining), 1 µg/mL (for nuclear staining, HCl-treated)  
IH　10 µg/mL (For nuclear staining, heat treatment or HCl treatment is necessary.)  
ELISA　Inhibition ELISA (Info. from the licenser. Not tested by MBL.)  
DB　Can be used.  
Other(Immuno-Nothern blotting)　Can be used.  （PMID: 26606401）
免疫原(抗原) KLH-conjugated 5-methylcytidine (KLH-m5C)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
別称 m5Cyd
背景 5-Methylcytosine (5mC), a modified nucleobase derived from cytosine, is present in both DNA and RNA molecules. 5mC in DNA is an essential epigenetic mark associated with gene silencing and genome stability during embryonic development. 5mC in ribonucleoside, called 5-methylcytidine (m5C), is involved in structural stability, aminoacylation and codon recognition of tRNA. In rRNA, m5C affects translational fidelity and tRNA recognition. m5C-modified nucleobase also have been identified in mRNA and other non-coding RNA but the biological functions are still unclear. m5C is detected not only in tissues and cells but also in urine. It is reported that the levels of m5C change in urine or tissues from patients with various diseases such as bladder, breast and colon cancers.
関連製品 D345-3 Anti-1-methyladenosine (m1A) mAb
PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb
MI-11-3 Anti-Bromodeoxyuridine mAb
M076-3 Mouse IgG2a (isotype control)
PM085 Anti-ZBTB24 pAb
PM086 Anti-ZBTB24 (Human) pAb
D347-3 Anti-Pseudouridine mAb
M227-3 Anti-Digoxigenin (DIG) mAb
M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb
M228-3 Anti-FITC mAb
RN019M Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
RN016M Anti-7-methylguanosine (m7G)-Cap mAb
RN017M Anti-7-methylguanosine (m7G) mAb
D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb
M202-3 Anti-Np95 (Uhrf1) mAb
RN127PW Anti-NSUN2 (Human) pAb
PM098 Anti-Inosine pAb
製品カテゴリー 分野:RNA-RNPネットワーク エピジェネティクス
データ





使用文献

Dot Blotting

  1. Garvilles RG et al. Dual Functions of the RFTS Domain of Dnmt1 in Replication-Coupled DNA Methylation and in Protection of the Genome from Aberrant Methylation. PLoS One 10, e0137509 (2015)(PMID:26383849)

Immunofluorescence

  1. Totsuka T et al. Production of mouse androgenetic embryos using spindle perturbation. Sci Rep. 10, 6556 (2020)(PMID:32300113)

Other(Immuno-Nothern blotting)

  1. Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
参考文献
  1. Seki Y et al. Extensive and orderly reprogramming of genome-wide chromatin modifications associated with specification and early development of germ cells in mice. Dev Biol. 278, 440-58 (2005)（PMID: 15680362）
  2. Sakai Y et al. Expression of DNA methyltransferase (Dnmt1) in testicular germ cells during development of mouse embryo. Cell Struct Funct. 26, 685-91 (2001)（PMID: 11942627）
  3. Mizugaki M et al. Preparation of a monoclonal antibody specific for 5-methyl-2'-deoxycytidine and its application for the detection of DNA methylation levels in human peripheral blood cells. Biol Pharm Bull. 19, 1537-40 (1996)（PMID: 8996634）

※在庫につきましては2024年04月19日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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