抗体 製品詳細情報

Code No. D153-3
Anti-GFP (Green Fluorescent Protein) mAb
価格(税別) ¥63,800
包装 100 µL (1 mg/mL)
在庫 10以上
（2024/04/20 00:05時点）
クローナリティー Monoclonal
クローン RQ2
アイソタイプ
(免疫動物)		 Rat IgG2aκ
使用法 IP　0.5-2 µg/sample  
IC　2 µg/mL  
免疫原(抗原) GFP purified from GFP expressed 293T cells by affinity chromatographic technique using mouse anti-GFP
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
背景 Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. Anti-GFP (Green Fluorescent Protein) mAb (clone RQ2) can detect GFP fusion protein on Immunoprecipitation and immunocytochemistry.
関連製品 D153-8 Anti-GFP (Green Fluorescent Protein) mAb-Agarose
D153-A48 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor™ 488
D153-A59 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor™ 594
D153-A64 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor™ 647
M048-3 Anti-GFP (Green Fluorescent Protein) mAb
598 Anti-GFP (Green Fluorescent Protein) pAb
598-7 Anti-GFP pAb-HRP-DirecT
D153-10 Anti-GFP (Green Fluorescent Protein) mAb-Magnetic Agarose
D153-11 Anti-GFP (Green Fluorescent Protein) mAb-Magnetic Beads
D153-6 Anti-GFP mAb-Biotin
PM073 Anti-Renilla GFP pAb
製品カテゴリー ツール:エピトープタグ 蛍光タンパク
データ


使用文献

Western Blotting

  1. Zhou H et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 7, 10151 (2016)(PMID:26743940)
  2. Nishimura R et al. Solo and Keratin Filaments Regulate Epithelial Tubule Morphology. Cell Struct Funct. 43, 95-105 (2018)(PMID:29709890)
  3. Li M et al. p53 cooperates with SIRT6 to regulate cardiolipin de novo biosynthesis. Cell Death Dis. 9, 941 (2018)(PMID:30237540)

Co-IP

  1. Qi Q et al. Involvement of the N-terminal B-box domain of Arabidopsis BBX32 protein in interaction with soybean BBX62 protein. J Biol Chem. 287, 31482-93 (2012)(PMID:22801428)

Immunoprecipitation

  1. Obuse C et al. A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol. 6, 1135-41 (2004)(PMID:15502821)
  2. Sakurai T et al. Membrane microdomain switching: a regulatory mechanism of amyloid precursor protein processing. J Cell Biol. 183, 339-52 (2008)(PMID:18936252)
  3. Sugiyama T et al. Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast. Nucleic Acids Res. 41, 6674-86 (2013)(PMID:23658229)
  4. Zhou H et al. Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. Nat Commun. 7, 10151 (2016)(PMID:26743940)
  5. Luteijn RD et al. Cowpox virus protein CPXV012 eludes CTLs by blocking ATP binding to TAP. J Immunol. 193, 1578-89 (2014)(PMID:25024387)
  6. Hatakeyama H et al. Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4-trafficking activities. J Biol Chem. 294, 1161-1172 (2019)(PMID:30482843)

Immunocytochemistry

  1. Kunishima S et al. ACTN1 mutations cause congenital macrothrombocytopenia. Am J Hum Genet. 92, 431-8 (2013)(PMID:23434115)
  2. Yamanaka T et al. Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci Rep. 10, 7610 (2020)(PMID:32376919)

Immunohistochemistry

  1. Matsuoka S et al. gone early, a novel germline factor, ensures the proper size of the stem cell precursor pool in the Drosophila ovary. PLoS One. 9, e113423 (2014)(PMID:25420147)
  2. Okazaki H et al. Calcineurin knockout mice show a selective loss of small spines. Neurosci Lett. 671, 99-102 (2018)(PMID:29427598)
  3. Urata Y et al. Spatio-temporal neural stem cell behavior leads to both perfect and imperfect structural brain regeneration in adult newts. Biol Open 7, bio033142 (2018)(PMID:29903864)

※在庫につきましては2024年04月20日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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