抗体 製品詳細情報

Code No. 598 プリントプリント
Anti-GFP (Green Fluorescent Protein) pAb
This antibody reacts with GFP, EBFP, SEBFP, ECFP, SECFP, EGFP, SEGFP, cpSEGFP, EYFP, Venus, cpVenus, R-pericam, and Sapphire.
価格(税別) ¥48,000
包装 100 µL
在庫 10以上
(2019/08/22 00:05時点)
クローナリティー Polyclonal
クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit IgG
使用法 WB 1:1,000-1:5,000  
IP 1 µL/sample  
IC 1:500  
IH 1:1,000-1:2,000  
ChIP* reported.  (PMID: 20345601)
Other(Immuno-EM) reported.  (PMID: 21949375 / 28771534)
免疫原(抗原) Recombinant GFP
性状 PBS/50% glycerol, pH 7.2
保存温度 -20°C
標識 Unlabeled
メーカー MBL
背景 Since the detection of intracellular Aequorea Victria Green Fluorescent Protein (GFP) requires only irradiation by UV or blue light, it provides an excellent means for monitoring gene expression and protein localization in living cells. The polyclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.
関連製品 598-7 Anti-GFP pAb-HRP-DirecT
M048-3 Anti-GFP (Green Fluorescent Protein) mAb
D153-3 Anti-GFP (Green Fluorescent Protein) mAb
D153-8 Anti-GFP (Green Fluorescent Protein) mAb-Agarose
D153-10 Anti-GFP (Green Fluorescent Protein) mAb-Magnetic Agarose
D153-A48 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor® 488
D153-A59 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor® 594
D153-A64 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor® 647
D153-11 Anti-GFP (Green Fluorescent Protein) mAb-Magnetic Beads
PM073 Anti-Renilla GFP pAb
製品カテゴリー ツール:エピトープタグ 蛍光タンパク
ブランド:使用文献・データ募集プレゼント対象製品
データ





使用文献
  1. Wang X et al. Girdin/GIV regulates collective cancer cell migration by controlling cell adhesion and cytoskeletal organization. Cancer Sci. 109, 3643-3656 (2018)

Western Blotting

  1. Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482)
  2. Maiuolo J et al. Selective activation of the transcription factor ATF6 mediates endoplasmic reticulum proliferation triggered by a membrane protein. PNAS. 108, 7832-7 (2011)(PMID:21521793)
  3. Watanabe H et al. Mouse sperm undergo GPI-anchored protein release associated with lipid raft reorganization and acrosome reaction to acquire fertility. J Cell Sci. 124, 2573-81 (2011)(PMID:21750187)
  4. Nakayama H et al. Human antigen R-mediated mRNA stabilization is required for ultraviolet B-induced autoinduction of amphiregulin in keratinocytes. J Biol Chem. 288, 10338-48 (2013)(PMID:23430747)
  5. Suzuki T et al. Development and disintegration of tapetum-specific lipid-accumulating organelles, elaioplasts and tapetosomes, in Arabidopsis thaliana and Brassica napus. Plant Sci. 207, 25-36 (2013)(PMID:23602096)
  6. Kawahara H et al. Tumor endothelial cell-specific drug delivery system using apelin-conjugated liposomes. PLoS One. 8, e65499 (2013)(PMID:23799018)
  7. Lee H et al. Zinc-finger antiviral protein mediates retinoic acid inducible gene I-like receptor-independent antiviral response to murine leukemia virus. PNAS. 110, 12379-84 (2013)(PMID:23836649)
  8. Ogawa Y et al. Elavl3 is essential for the maintenance of Purkinje neuron axons. Sci Rep. 8, 2722 (2018)(PMID:29426875)
  9. Okabe T et al. BiFC-based visualisation system reveals cell fusion morphology and heterokaryon incompatibility in the filamentous fungus Aspergillus oryzae. Sci Rep. 8, 2922 (2018)(PMID:29440689)
  10. Ito S et al. CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells. Oncotarget 9, 17645-17655 (2018)(PMID:29707137)
  11. Nabeshima R et al. Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters. Elife 7, e3643 (2018)(PMID:30070635)
  12. Wang H et al. A potato STRUBBELIG-RECEPTOR FAMILY member, StLRPK1, associates with StSERK3A/BAK1 and activates immunity. J Exp Bot. 69, 5573-5586 (2018)(PMID:30137408)
  13. Wang X et al. Girdin/GIV regulates collective cancer cell migration by controlling cell adhesion and cytoskeletal organization. Cancer Sci. 109, 3643-3656 (2018) (PMID:30194792)
  14. Duan GF et al. Signal peptide represses GluK1 surface and synaptic trafficking through binding to amino-terminal domain. Nat Commun. 9, 4879 (2018) (PMID:30451858)
  15. Kiuchi T et al. Two CCCH-type zinc finger domains in the Masc protein are dispensable for masculinization and dosage compensation in Bombyx mori. Insect Biochem Mol Biol. 104, 30-38 (2019)(PMID:30529581)
  16. Cao J et al. RACK1 Promotes Self-Renewal and Chemoresistance of Cancer Stem Cells in Human Hepatocellular Carcinoma through Stabilizing Nanog. Theranostics. 9, 811-828 (2019)(PMID:30809310)

ChIP

  1. Fukazawa J et al. The transcription factor RSG regulates negative feedback of NtGA20ox1 encoding GA 20-oxidase. Plant J. 62, 1035-45 (2010)(PMID:20345601)
  2. Ono S et al. EAT1 transcription factor, a non-cell-autonomous regulator of pollen production, activates meiotic small RNA biogenesis in rice anther tapetum. PLoS Genet. 14, e1007238 (2018)(PMID:29432414)
  3. Nabeshima R et al. Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters. Elife 7, e3643 (2018)(PMID:30070635)

Immunoprecipitation

  1. Suzuki M et al. MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization. Development. 137, 2329-39 (2010)(PMID:20534674)
  2. Abudureyimu S et al. Essential Role of Linx/Islr2 in the Development of the Forebrain Anterior Commissure. Sci Rep. 8, 7292 (2018)(PMID:29739947)
  3. Yang L et al. Reconstituting Arabidopsis CRY2 Signaling Pathway in Mammalian Cells Reveals Regulation of Transcription by Direct Binding of CRY2 to DNA. Cell Rep. 24, 585-593.e4(2018)(PMID:30021157)
  4. Nabeshima R et al. Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters. Elife 7, e3643 (2018)(PMID:30070635)
  5. Hisamoto N et al. Phosphatidylserine exposure mediated by ABC transporter activates the integrin signaling pathway promoting axon regeneration. Nat Commun. 9, 3099 (2018)(PMID:30082731)
  6. Matsumoto A et al. Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer. Methods Mol Biol. 1840, 307-319 (2018)(PMID:30141053)
  7. Wang X et al. Girdin/GIV regulates collective cancer cell migration by controlling cell adhesion and cytoskeletal organization. Cancer Sci. 109, 3643-3656 (2018) (PMID:30194792)
  8. Duan GF et al. Signal peptide represses GluK1 surface and synaptic trafficking through binding to amino-terminal domain. Nat Commun. 9, 4879 (2018) (PMID:30451858)
  9. Hatakeyama H et al. Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4-trafficking activities. J Biol Chem. 294, 1161-1172 (2019) (PMID:30482843)

Flow Cytometry

  1. Muraoka N et al. Role of cyclooxygenase-2-mediated prostaglandin E2-prostaglandin E receptor 4 signaling in cardiac reprogramming. Nat Commun. 10, 674 (2019)(PMID:30787297)

Immunocytochemistry

  1. Jeyasekharan AD et al. DNA damage regulates the mobility of Brca2 within the nucleoplasm of living cells. PNAS. 107, 21937-42 (2010)(PMID:21098284)
  2. Werner M et al. 2'-O-ribose methylation of cap2 in human: function and evolution in a horizontally mobile family. Nucleic Acids Res. 39, 4756-68 (2011)(PMID:21310715)
  3. Kigoshi Y et al. Ubiquitin ligase activity of Cul3-KLHL7 protein is attenuated by autosomal dominant retinitis pigmentosa causative mutation. J Biol Chem. 286, 33613-21 (2011)(PMID:21828050)
  4. Yun J et al. Neuronal Per Arnt Sim (PAS) domain protein 4 (NPAS4) regulates neurite outgrowth and phosphorylation of synapsin I. J Biol Chem. 288, 2655-64 (2013)(PMID:23172225)
  5. Arimoto-Matsuzaki K et al. MyD88 NEDDylation negatively regulates MyD88-dependent NF-κB signaling through antagonizing its ubiquitination. Nat Commun. 7, 10252 (2016)(PMID:26738979)
  6. Oguchi ME et al. TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One. 12, e017488 (2017)(PMID:28384198)
  7. Tomoshige S et al. Cytoskeleton-related regulation of primary cilia shortening mediated by melanin-concentrating hormone receptor 1. Gen Comp Endocrinol. 253, 44-52 (2017)(PMID:28842217)
  8. Kader MA et al. Molecular basis of the microtubule-regulating activity of microtubule crosslinking factor 1. PLoS One 12, e0182641 (2017)(PMID:28787032)
  9. Kim J et al. KLHL7 promotes TUT1 ubiquitination associated with nucleolar integrity: Implications for retinitis pigmentosa. Biochem Biophys Res Commun. 494, 220-226 (2017)(PMID:29032201)
  10. Slomnicki LP et al. Ribosomal stress and Tp53-mediated neuronal apoptosis in response to capsid protein of the Zika virus. Sci Rep. 7, 16652 (2017)(PMID:29192272)
  11. Shimozaki, K., Involvement of Nuclear Receptor REV-ERBβ in Formation of Neurites and Proliferation of Cultured Adult Neural Stem Cells. Cell Mol Neurobiol. 38, 1051-1065 (2018)(PMID:29397477)
  12. Tsuchiya M et al. Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Nat Commun. 9, 2049 (2018)(PMID:29799007)
  13. Kondo T et al. Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys. Cell Rep. 24, 2191-2195.e4. (2018)(PMID:30134178)

Immunohistochemistry

  1. Suzuki M et al. MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization. Development. 137, 2329-39 (2010)(PMID:20534674)
  2. Miyatsuka T et al. Neurogenin3 inhibits proliferation in endocrine progenitors by inducing Cdkn1a. PNAS. 108, 185-90 (2011)(PMID:21173230)
  3. London A et al. Neuroprotection and progenitor cell renewal in the injured adult murine retina requires healing monocyte-derived macrophages. J Exp Med. 208, 23-39 (2011)(PMID:21220455)
  4. Yokomizo T et al. Three-dimensional imaging of whole midgestation murine embryos shows an intravascular localization for all hematopoietic clusters. Blood 117, 6132-4 (2011)(PMID:21505195)
  5. Wang Y et al. Girdin is an intrinsic regulator of neuroblast chain migration in the rostral migratory stream of the postnatal brain. J Neurosci. 31, 8109-22 (2011)(PMID:21632933)
  6. Sekine K et al. The outermost region of the developing cortical plate is crucial for both the switch of the radial migration mode and the Dab1-dependent "inside-out" lamination in the neocortex. J Neurosci. 31, 9426-39 (2011)(PMID:21697392)
  7. Guo L et al. Stat3-coordinated Lin-28-let-7-HMGA2 and miR-200-ZEB1 circuits initiate and maintain oncostatin M-driven epithelial-mesenchymal transition. Oncogene 32, 5272-82 (2013)(PMID:23318420)
  8. Kaneko A et al. Damage-induced neuronal endopeptidase (DINE) enhances axonal regeneration potential of retinal ganglion cells after optic nerve injury. Cell Death Dis. 8, e2847 (2017)(PMID:28569783)
  9. Takara K et al. Lysophosphatidic Acid Receptor 4 Activation Augments Drug Delivery in Tumors by Tightening Endothelial Cell-Cell Contact. Cell Rep. 20, 2072-2086 (2017)(PMID:28854359)
  10. Yamasaki T et al. Age-dependent motor dysfunction due to neuron-specific disruption of stress-activated protein kinase MKK7. Sci Rep. 7, 7348 (2017)(PMID:28779160)
  11. Miura Y, Kanaho Y. ACAP3, the GTPase-activating protein specific to the small GTPase Arf6, regulates neuronal migration in the developing cerebral cortex. Biochem Biophys Res Commun. 493, 1089-1094 (2017)(PMID:28919417)
  12. Matsuda K et al. Granule cells control recovery from classical conditioned fear responses in the zebrafish cerebellum. Sci Rep. 7, 11865 (2017)(PMID:28928404)
  13. Deczkowska A et al. Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner. Nat Commun. 8, 717 (2017)(PMID:28959042)
  14. Morito N et al. Transcription factor MafB may play an important role in secondary hyperparathyroidism. Kidney Int. 93, 54-68 (2017)(PMID:28964572)
  15. Hasegawa Y et al. Luciferase shRNA Presents off-Target Effects on Voltage-Gated Ion Channels in Mouse Hippocampal Pyramidal Neurons. eNeuro. 4, e0186-17.2017 1–16 (2017)(PMID:29034317)
  16. Mori T et al. SOX10 Expression as Well as BRAF and GNAQ/11 Mutations Distinguish Pigmented Ciliary Epithelium Neoplasms From Uveal Melanomas. Invest Ophthalmol Vis Sci. 58, 5445-5451 (2017)(PMID:29059311)
  17. Ogawa Y et al. Elavl3 is essential for the maintenance of Purkinje neuron axons. Sci Rep. 8, 2722 (2018)(PMID:29426875)
  18. Nakatomi M et al. Nestin expression is differently regulated between odontoblasts and the subodontoblastic layer in mice. Histochem Cell Biol. 149, 383-391 (2018)(PMID:29445893)
  19. Kendall GC et al. AX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis. Elife 7, e33800 (2018)(PMID:29869612)
  20. Ojima N et al. Genetic dissection of stress-induced reproductive arrest in Drosophila melanogaster females. PLoS Genet. 14, e1007434 (2018)(PMID:29889831)
  21. Nabeshima R et al. Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters. Elife 7, e3643 (2018)(PMID:30070635)
  22. Sugiyama Y et al. Intravenous Administration of Bone Marrow-Derived Mesenchymal Stem Cell, but not Adipose Tissue-Derived Stem Cell, Ameliorated the Neonatal Hypoxic-Ischemic Brain Injury by Changing Cerebral Inflammatory State in Rat. Front Neurol. 9, 757 (2018)(PMID:30254603)
  23. Bin Saifullah MA et al. Cell type-specific activation of mitogen-activated protein kinase in D1 receptor-expressing neurons of the nucleus accumbens potentiates stimulus-reward learning in mice. Sci Rep. 8, 14413 (2018)(PMID:30258218)
  24. Plein A et al. Erythro-myeloid progenitors contribute endothelial cells to blood vessels. Nature 562, 223-228 (2018)(PMID:30258231)
  25. Tanaka J et al. Generation of orthotopically functional salivary gland from embryonic stem cells. Nat Commun. 9, 4216 (2018)(PMID:30310071)
  26. Shimauchi-Ohtaki H et al. The dynamics of revascularization after white matter infarction monitored in Flt1-tdsRed and Flk1-GFP mice. Neurosci Lett. 692, 70-76 (2019)(PMID:30389418)
  27. Shirahase T et al. Ethanol Preference and Drinking Behavior Are Controlled by RNA Editing in the Nucleus Accumbens. Front Behav Neurosci. 12, 331 (2019)(PMID:30697154)
  28. Rosenzweig N et al. PD-1/PD-L1 checkpoint blockade harnesses monocyte-derived macrophages to combat cognitive impairment in a tauopathy mouse model. Nat Commun. 10, 465 (2019) (PMID:30692527)

Immunofluorescence

  1. Watanabe K et al. The Cep57-pericentrin module organizes PCM expansion and centriole engagement. Nat Commun. 10, 931 (2019)(PMID:30804344)

Other(Immuno-EM)

  1. Nori S et al. Grafted human-induced pluripotent stem-cell–derived neurospheres promote motor functional recovery after spinal cord injury in mice. PNAS 108, 16825-30 (2011)(PMID:21949375)
  2. Suzuki H et al. Neural stem cell mediated recovery is enhanced by Chondroitinase ABC pretreatment in chronic cervical spinal cord injury. PLoS One 12, e0182339 (2017)(PMID:28771534)
  3. Arii J et al. ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity. Nat Commun. 9, 3379 (2018)(PMID:30139939)

※在庫につきましては2019年08月22日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation,
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization

ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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