Anti-PCBP2 pAb

価格（税別）

¥66,000

在庫

1-9

（2024/04/19 17:00時点）

包装

200 µL (1 mg/mL)

使用文献

データシート

SDS

データ	Western Blotting Immunoprecipitation RNP Immunoprecipitation
クローナリティー	Polyclonal	クローン	Polyclonal
アイソタイプ（免疫動物）	Rabbit Ig (aff.)
使用法	WB 1 µg/mL IP 5 µg/500 µL of cell extract from 5 x 10⁶ cells IC* reported. （PMID: 26990993） RIP 15 µg/500 µL of cell extract from 1.5 x 10⁷ cells Other(Immunodepletion) reported. （PMID: 22438951） Other(eCLIP) reported. （PMID: 27018577）
免疫原(抗原)	KLH conjugated synthetic peptide, corresponding to internal region of human PCBP2
交差性 [Gene ID]	Human[5094](PMID: 22438951), Mouse[18521], Rat[363005]
性状	1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度	-20°C	標識	Unlabeled	メーカー	MBL
別称	poly(rC) binding protein 2, HNRPE2, hnRNP-E2
背景	Poly(rC)-binding proteins (PCBPs) are cellular RNA-binding proteins, characterized by their triple KH structure and by their poly(C)-binding specificity. The 3 KH domains are not only involved in nucleic acid binding but also mediate protein-protein interactions within the RNP complexes. It binds specifically to polypyrimidine tracts within the 3' UTR of target mRNAs through KH domains. PCBPs are expressed as 4 isoforms in cells. PCBP1 and PCBP2 are predominantly expressed in the nucleus and can shuttle from the nucleus to the cytoplasm, while PCBP3 and PCBP4 are expressed to a less extent in the cytoplasm. The most characterized gene regulated by PCBP1 and PCBP2 is the a-globin gene, and the accumulated α-globin mRNA is known to account for approximately 95% of total cellular mRNA during terminal erythroid differentiation. Specific binding of PCBPs to 3′ UTR of mRNA is associated with the stabilization of a-globin mRNA. Microarray analysis of human mRNAs present in a specific subset of PCBP2-RNP complexes also showed a potential involvement of PCBP2 in transcription factor expression via association with their mRNA; further, the enrichment of PCBP2 mRNA in PCBP2-RNP complexes suggested that PCBP2 expression may be subject to posttranscriptional autoregulation.
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使用文献	使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。 Western Blotting Neff AT et al. Global analysis reveals multiple pathways for unique regulation of mRNA decay in induced pluripotent stem cells. Genome Res. 1457-67 (2012)(PMID:22534399) Yabe-Wada T et al. PCBP2 Post-Transcriptionally Regulates Sortilin Expression by Binding to a C-rich Element in Its 3' UTR. FEBS Open Bio. 10, 407-413 (2020)(PMID:31961070) Immunocytochemistry Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993) Markmiller S et al. Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules. Cell. 172, 590-604.e13 (2018)(PMID:29373831) RNP Immunoprecipitation Palusa S et al. The 3' untranslated region of the rabies virus glycoprotein mRNA specifically interacts with cellular PCBP2 protein and promotes transcript stability. PLoS One. 7, e33561 (2012)(PMID:22438951) Yabe-Wada T et al. PCBP2 Post-Transcriptionally Regulates Sortilin Expression by Binding to a C-rich Element in Its 3' UTR. FEBS Open Bio. 10, 407-413 (2020)(PMID:31961070) Other(Immunodepletion) Palusa S et al. The 3' untranslated region of the rabies virus glycoprotein mRNA specifically interacts with cellular PCBP2 protein and promotes transcript stability. PLoS One. 7, e33561 (2012)(PMID:22438951) Other(eCLIP) Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993) Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
製品カテゴリー	分野 RNA-RNPネットワークブランド RiboCluster Profiler™

※在庫につきましては2024年04月19日 17時00分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
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※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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