トップ > 製品検索結果一覧 > Code No. D346-3 Anti-5-methylcytidine (m5C) mAb

Code No. D346-3

Anti-5-methylcytidine (m5C) mAb

価格(税別)

¥60,000

在庫

10以上

(2017/07/25 01:30時点)

包装

100 µg/100 µL

Anti-5-methylcytidine (m5C) mAb (Clone: FMC-9) reacts with 5-methylcytidine (m5C or m5Cyd), 5-methyl-2’-deoxycytidine (m5dC or m5dCyd) and 5-methylcytosine (5mC, 5-mC or m5Cyt).
データ
  • Immunoprecipitation

    MeDIP was performed using mouse genomic DNA fragments containing synthesized DNAs. IP'd samples were analyzed by real-time qPCR using primers specific to synthetic DNA spike-in controls or Loci 1-3.

  • Immunocytochemistry

  • Immunohistochemistry

  • Immunohistochemistry

  • Dot Blotting

クローナリティー Monoclonal クローン FMC-9
アイソタイプ
(免疫動物)
Mouse IgG2aλ
使用法
IP
Can be used.  
IC
5 µg/mL (for cytoplasmic staining), 1 µg/mL (for nuclear staining, HCl-treated)  
IH
10 µg/mL (For nuclear staining, heat treatment or HCl treatment is necessary.)  
ELISA
Inhibition ELISA (Info. from the licenser. Not tested by MBL.)  
DB
Can be used.  
Other(Immuno-Nothern blotting)
Can be used.  (PMID: 26606401
免疫原(抗原) KLH-conjugated 5-methylcytidine (KLH-m5C)
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 m5Cyd
背景 5-Methylcytosine (5mC), a modified nucleobase derived from cytosine, is present in both DNA and RNA molecules. 5mC in DNA is an essential epigenetic mark associated with gene silencing and genome stability during embryonic development. 5mC in ribonucleoside, called 5-methylcytidine (m5C), is involved in structural stability, aminoacylation and codon recognition of tRNA. In rRNA, m5C affects translational fidelity and tRNA recognition. m5C-modified nucleobase also have been identified in mRNA and other non-coding RNA but the biological functions are still unclear. m5C is detected not only in tissues and cells but also in urine. It is reported that the levels of m5C change in urine or tissues from patients with various diseases such as bladder, breast and colon cancers.
関連製品 D345-3 Anti-1-methyladenosine (m1A) mAb
PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb
MI-11-3 Anti-Bromodeoxyuridine mAb
M076-3 Mouse IgG2a (isotype control)
5270-100 MethylHunter MBD1-based Methylated DNA Enrichment Kit
5275-100 MethylHunter MBD1-based Methylated DNA Enrichment Kit 2
PM085 Anti-ZBTB24 pAb
PM086 Anti-ZBTB24 (Human) pAb
D347-3 Anti-Pseudouridine mAb
M227-3 Anti-Digoxigenin (DIG) mAb
M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb
M229-3 Anti-MECP2 mAb
M228-3 Anti-FITC mAb
RN019M Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
RN131P Anti-N6-methyladenosine (m6A) pAb
RN016M Anti-7-methylguanosine (m7G)-Cap mAb
RN017M Anti-7-methylguanosine (m7G) mAb
5270-200 MethylHunter MBD1-based Methylated DNA Enrichment Kit
5275-200 MethylHunter MBD1-based Methylated DNA Enrichment Kit 2
D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb
M202-3 Anti-Np95 (Uhrf1) mAb
RN127PW Anti-NSUN2 (Human) pAb
PM098 Anti-Inosine pAb
使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Dot Blotting

  1. Garvilles RG et al. Dual Functions of the RFTS Domain of Dnmt1 in Replication-Coupled DNA Methylation and in Protection of the Genome from Aberrant Methylation. PLoS One 10, e0137509 (2015)(PMID:26383849)

Other(Immuno-Nothern blotting)

  1. Mishima E et al. Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. PLoS One 10, e0143756 (2015)(PMID:26606401)
参考文献
  1. Seki Y et al. Extensive and orderly reprogramming of genome-wide chromatin modifications associated with specification and early development of germ cells in mice. Dev Biol. 278, 440-58 (2005)(PMID: 15680362
  2. Sakai Y et al. Expression of DNA methyltransferase (Dnmt1) in testicular germ cells during development of mouse embryo. Cell Struct Funct. 26, 685-91 (2001)(PMID: 11942627
  3. Mizugaki M et al. Preparation of a monoclonal antibody specific for 5-methyl-2'-deoxycytidine and its application for the detection of DNA methylation levels in human peripheral blood cells. Biol Pharm Bull. 19, 1537-40 (1996)(PMID: 8996634
製品
カテゴリー
分野
RNA-RNPネットワーク
エピジェネティクス
ブランド
使用文献プレゼント対象製品
この製品に関するお問い合わせをする

※在庫につきましては2017年07月25日 01時30分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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