トップ > 製品検索結果一覧 > Code No. D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb

Code No. D289-3

Anti-Np95 (Uhrf1) (Mouse) mAb

価格(税別)

¥48,000

在庫

10以上

(2019/08/22 00:05時点)

包装

100 µg/100 µL

データ
  • Western Blotting

  • Immunocytochemistry

クローナリティー Monoclonal クローン Th-10a
アイソタイプ
(免疫動物)
Rat IgG2aκ
使用法
WB
1 µg/mL  
IP*
reported.  (PMID: 8634372
FCM*
reported.  (PMID: 8634372
IC
2 µg/mL  
IH*
reported.  (PMID: 8634372
免疫原(抗原) Mouse thymic lymphoma
交差性
[Gene ID]

Human(-), Mouse[18140], Rat(-), Hamster(-)

性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 ICBP90, RNF106, AL022808
背景 DNA methylation inheritance is the process of copying pre-existing methylation patterns onto newly replicated DNA strand after DNA replication. Dnmt1 is the maintenance DNA methyltransferase, which methylates hemimethylated CpGs that appear after DNA repairs and replications. The mouse Np95, also known as Uhrf1, is a nuclear protein, which contains an ubiquitin-like domain, a tandem Tudor domain, a PHD (Plant Homeodomain) finger, an SRA (SET-and RING-associated) domain, and a RING (Really Interesting New Gene) finger domain. Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.
関連製品 M202-3 Anti-Np95 (Uhrf1) mAb
MK-15-3 Anti-Rb (Human) mAb
MH-12-3 Anti-PCNA (Proliferating Cell Nuclear Antigen) mAb
M081-3 Rat IgG2a (isotype control)
M207-3 Anti-MitoPLD (Pld6) mAb
PM044 Anti-PIWIL2 (MILI) (Mouse) pAb
PM043 Anti-PIWIL2 (MILI) (Mouse) pAb
RN1005 RIP-Assay Kit for microRNA
PM085 Anti-ZBTB24 pAb
PM086 Anti-ZBTB24 (Human) pAb
D346-3 Anti-5-methylcytidine (m5C) mAb
使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Fujimori A et al. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. Mamm Genome. 9, 1032-5 (1998)(PMID:9880673)
  2. Bonapace IM et al. Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. J Cell Biol. 157, 909-14 (2002)(PMID:12058012)

Immunoprecipitation

  1. Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995)(PMID:8634372)

Flow Cytometry

  1. Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995)(PMID:8634372)

Immunocytochemistry

  1. Uemura T et al. Temporal and spatial localization of novel nuclear protein NP95 in mitotic and meiotic cells. Cell Struct Funct. 25, 149-59 (2000)(PMID:10984098)
  2. Bonapace IM et al. Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. J Cell Biol. 157, 909-14 (2002)(PMID:12058012)
  3. Sharif J et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature. 450, 908-12 (2007)(PMID:17994007)
  4. Ohno R et al. A replication-dependent passive mechanism modulates DNA demethylation in mouse primordial germ cells. Development 140, 2892-903 (2013)(PMID:23760957)

Immunohistochemistry

  1. Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995)(PMID:8634372)
  2. Fujimori A et al. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. Mamm Genome. 9, 1032-5 (1998)(PMID:9880673)
  3. Maenohara S et al. Role of UHRF1 in de novo DNA methylation in oocytes and maintenance methylation in preimplantation embryos. PLoS Genet. 13, e1007042 (2017) 
     (PMID:28976982)
製品
カテゴリー
分野
エピジェネティクス
がん
この製品に関するお問い合わせをする

※在庫につきましては2019年08月22日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。