トップ > 製品検索結果一覧 > Code No. PM045 Anti-p62 (SQSTM1) pAb

Code No. PM045

Anti-p62 (SQSTM1) pAb

価格(税別)

¥38,000

在庫

10以上

(2017/07/25 01:30時点)

包装

100 µL

データ
  • Western Blotting

  • Immunoprecipitation

  • Immunocytochemistry

  • Immunohistochemistry

クローナリティー Polyclonal クローン Polyclonal
アイソタイプ
(免疫動物)
Rabbit Ig (aff.)
使用法
WB
1:1,000  
IP
2 µL/300 µL of cell extract from 1x107 cells  
IC
1:500  
IH
1:1,000 (Heat treatment is necessary for paraffin embedded sections.)  
免疫原(抗原) Recombinant Human p62 (120-440 a.a.)
交差性
[Gene ID]

Human[8878], Mouse[18412], Rat[113894], Hamster[100768352]

性状 PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 sequestosome 1, p60, p62, A170, OSIL, PDB3, ZIP3, p62B/Osi, A170, STAP, OSF-6/Osi, ZIP
背景 p62/SQSTM1 interacts with various molecular groups such as RIP, TRAF6, ERK, aPKCs, and poly-ubiquitin through PB1 domain, Zn finger domain, and UBA domain. This protein directly interacts with LC3, which is localized on autophagosome membrane, and is degradated by autophagic-lysosome pathway. p62 regulates ubiquitin-positive protein aggregates caused by autophagy deficiency.
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M186-3 Anti-LC3 mAb
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M217-3 Anti-Phospho-p62 (SQSTM1) (Ser351) mAb
M217-3MS Anti-Phospho-p62 (SQSTM1) (Ser351) mAb for My select sampler set
M224-3 Anti-KEAP1 mAb
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CY-7055 CycLex® Total p62 ELISA Kit
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CY-7057 CycLex® Phospho-p62 Ser403 ELISA Kit
使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Waguri S, Komatsu M. Biochemical and morphological detection of inclusion bodies in autophagy-deficient mice. Methods Enzymol. 453, 181-96 (2009)(PMID:19216907)
  2. Otomo T et al. Inhibition of autophagosome formation restores mitochondrial function in mucolipidosis II and III skin fibroblasts. Mol Genet Metab. 98, 393-9 (2009)(PMID:19656701)
  3. Ogata T et al. Fasting-related autophagic response in slow- and fast-twitch skeletal muscle. Biochem Biophys Res Commun. 394, 136-40 (2010)(PMID:20184860)
  4. Itakura E, Mizushima N. p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J Cell Biol. 192, 17-27 (2011)(PMID:21220506)
  5. Lee EJ, Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy 7 689-95  (2011)(PMID:21460635)
  6. Higaki K et al. Chemical chaperone therapy: chaperone effect on mutant enzyme and cellular pathophysiology in β-galactosidase deficiency. Hum Mutat. 32, 843-52 (2011)(PMID:21520340)
  7. Myeku N, Figueiredo-Pereira ME. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem. 286, 22426-40 (2011)(PMID:21536669)
  8. Takamura A et al. Lysosomal accumulation of Trk protein in brain of GM1 -gangliosidosis mouse and its restoration by chemical chaperone. J Neurochem. 118, 399-406 (2011)(PMID:21574998)
  9. Huang Y et al. PML-RARα enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway. Autophagy 7, 1132-44 (2011)(PMID:21673516)
  10. Osawa Y et al. L-tryptophan-mediated enhancement of susceptibility to nonalcoholic fatty liver disease is dependent on the mammalian target of rapamycin. J Biol Chem. 286, 34800-8 (2011)(PMID:21841000)
  11. Frankel LB et al. microRNA-101 is a potent inhibitor of autophagy. EMBO J. 30, 4628-41 (2011)(PMID:21915098)
  12. Takaesu G et al. TGFβ-activated kinase 1 (TAK1)-binding proteins (TAB) 2 and 3 negatively regulate autophagy. J Biochem. 151, 157-66 (2012)(PMID:21976705)
  13. Matsumoto G et al. Serine 403 Phosphorylation of p62/SQSTM1 Regulates Selective Autophagic Clearance of Ubiquitinated Proteins. Mol Cell 44, 279-89 (2011)(PMID:22017874)
  14. Berliocchi L et al. Autophagy impairment in a mouse model of neuropathic pain. Mol Pain 7, 83 (2011)(PMID:22023914)
  15. Rovetta F et al. ER signaling regulation drives the switch between autophagy and apoptosis in NRK-52E cells exposed to cisplatin. Exp Cell Res. 318, 238-50 (2012)(PMID:22146761)
  16. Wu SY et al. Ras-related tumorigenesis is suppressed by BNIP3-mediated autophagy through inhibition of cell proliferation. Neoplasia 13, 1171-82 (2011)(PMID:22241963)
  17. Takasaka N et al. Autophagy induction by SIRT6 through attenuation of insulin-like growth factor signaling is involved in the regulation of human bronchial epithelial cell senescence. J Immunol. 192, 958-68 (2014)(PMID:24367027 )
  18. Hasegawa J et al. Autophagosome-lysosome fusion in neurons requires INPP5E, a protein associated with Joubert syndrome. EMBO J. 35, 1853-67 (2016)(PMID:27340123)
  19. Takayama K et al. Dissection of ubiquitinated protein degradation by basal autophagy. FEBS Lett. 591, 1199-1211 (2017)(PMID:28369861)

Immunocytochemistry

  1. Dupont N et al. Shigella phagocytic vacuolar membrane remnants participate in the cellular response to pathogen invasion and are regulated by autophagy. Cell Host Microbe. 6, 137-49 (2009)(PMID:19683680)
  2. Itakura E, Mizushima N. p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J Cell Biol. 192, 17-27 (2011)(PMID:21220506)
  3. Higaki K et al. Chemical chaperone therapy: chaperone effect on mutant enzyme and cellular pathophysiology in β-galactosidase deficiency. Hum Mutat. 32, 843-52 (2011)(PMID:21520340)
  4. Myeku N, Figueiredo-Pereira ME. Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62. J Biol Chem. 286, 22426-40 (2011)(PMID:21536669)
  5. Huang C et al. Preconditioning involves selective mitophagy mediated by Parkin and p62/SQSTM1. PLoS One 6, e20975 (2011)(PMID:21687634)
  6. Seto S et al. Coronin-1a inhibits autophagosome formation around Mycobacterium tuberculosis-containing phagosomes and assists mycobacterial survival in macrophages. Cell Microbiol. 14, 710-27 (2012)(PMID:22256790)
  7. Sano O et al. Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition. FEBS Lett. 590, 1576-85 (2016)(PMID:27135648)
  8. Takayama K et al. Dissection of ubiquitinated protein degradation by basal autophagy. FEBS Lett. 591, 1199-1211 (2017)(PMID:28369861)

Immunohistochemistry

  1. Waguri S, Komatsu M. Biochemical and morphological detection of inclusion bodies in autophagy-deficient mice. Methods Enzymol. 453, 181-96 (2009)(PMID:19216907)
  2. Urbanczyk A et al. PKCζ-interacting protein ZIP3 is generated by intronic polyadenylation, and is expressed in the brain and retina of the rat. Biochem J. 433, 43-50 (2011)(PMID:20979579)
  3. Chen H et al. Titration of mitochondrial fusion rescues Mff-deficient cardiomyopathy. J Cell Biol. 211, 795-805 (2015)(PMID:26598616 )
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※在庫につきましては2017年07月25日 01時30分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。