Anti-PIWIL2 (MILI) (Mouse) pAb

価格（税別）

¥52,800

在庫

10以上

（2024/04/19 00:05時点）

包装

100 µL

使用文献

データシート

SDS

データ	Western Blotting
クローナリティー	Polyclonal	クローン	Polyclonal
アイソタイプ（免疫動物）	Rabbit Ig (aff.)
使用法	WB 1:1,000 IP 1 µL/sample IH* reported. （PMID: 29018194） RIP* reported. （PMID: 18381894 / 19735482 / 20439430）
免疫原(抗原)	synthetic peptide VRKDREEPRSSLPDPS corresponding to Mouse PIWIL2 (107-122 a.a.)
交差性 [Gene ID]	Mouse[57746]
性状	PBS/50% glycerol, pH 7.2
保存温度	-20°C	標識	Unlabeled	メーカー	MBL
背景	The Piwi/Argonaute protein family plays important roles in stem-cell self-renewal, RNA silencing and translational regulation in various organisms. The family members are defined by conserved both PAZ (Piwi Argonaut and Zwille) and Piwi domains participate in RNA binding. Piwi-interacting RNA (piRNA) is a class of small RNA molecules that is expressed uniquely in mammalian testes and forms RNA-protein complexes with Piwi proteins. These small regulatory RNAs have been implicated in spermatogenesis, repression of retrotransposon transposition in germline cells, epigenetic regulation and positive regulation of translation and mRNA stability. MIWI (PIWIL1) and MILI (PIWIL2) are mouse homologues of Piwi. MILI is expressed from primordial germ cells (PGC) in embryo, whereas MIWI is detected later from the mid-pachytene stage to early round spermatids. Their expression overlaps in the mid-pachytene and early round spermatid stage. The expression patterns and knockout phenotypes of mammalian Piwi members indicated MILI is an essential factor for meiotic differentiation during spermatogenesis.
関連製品	RN003M Anti-EIF2C2 (AGO2) (Human) mAb RN1005 RIP-Assay Kit for microRNA D128-3 Anti-Phospho-STAT3 (Tyr708) mAb M207-3 Anti-MitoPLD (Pld6) mAb D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb D356-3 Anti-Jmjd1c (Mouse) mAb M202-3 Anti-Np95 (Uhrf1) mAb RN125PW Anti-HENMT1 pAb D357-3 Anti-Ace (CD143) (Mouse) mAb D363-3 Anti-Ly6k (Mouse) mAb D365-3 Anti-PIWIL2 (MILI) (Mouse) mAb
使用文献	使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。 Western Blotting Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482) Kuramochi-Miyagawa S et al. MVH in piRNA processing and gene silencing of retrotransposons. Genes Dev. 24, 887-92 (2010)(PMID:20439430) Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194) Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670) Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019) (PMID:30590800) Immunoprecipitation Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482) Shiromoto Y et al. GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells. RNA 19, 803-10 (2013)(PMID:23611983) Immunohistochemistry Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194) Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670) Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019)(PMID:30590800) RNP Immunoprecipitation Kuramochi-Miyagawa S et al. DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. Genes Dev. 22, 908-17 (2008)(PMID:18381894) Kojima K et al. Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells. Genes Cells. 14, 1155-65 (2009)(PMID:19735482) Kuramochi-Miyagawa S et al. MVH in piRNA processing and gene silencing of retrotransposons. Genes Dev. 24, 887-92 (2010)(PMID:20439430) Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 8, 819 (2017)(PMID:29018194) Ding D et al. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 9, 127 (2017)(PMID:29317670) Nishimura T et al. PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep. 19, e44957 (2018)(PMID:29444933) Ding D et al. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 47, 2594-2608 (2019)(PMID:30590800)
製品カテゴリー	分野幹細胞研究 RNA-RNPネットワークエピジェネティクスがん

※在庫につきましては2024年04月19日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
※本サイトの掲載製品は、品質改良のため予告なく仕様などの変更、または販売中止となる場合がございます。あらかじめご了承ください。