トップ > 製品検索結果一覧 > Code No. M047-3 Anti-Myc-tag mAb

Code No. M047-3

Anti-Myc-tag mAb

価格(税別)

¥48,000

在庫

10以上

(2019/07/22 00:05時点)

包装

200 µg/100 µL

データ
  • Western Blotting

クローナリティー Monoclonal クローン PL14
アイソタイプ
(免疫動物)
Mouse IgG1 κ
使用法
WB
1 µg/mL  
IP
5 µg/sample  
IC
  
RIP*
reported.  (PMID: 23064114 / 26738979
ChIP*
reported.  (PMID: 19763945
免疫原(抗原) 6myc-tag fusion protein
性状 2 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
背景 Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
関連製品 M047-7 Anti-Myc-tag mAb-HRP-DirecT
M047-8 Anti-Myc-tag mAb-Agarose
M047-11 Anti-Myc-tag mAb-Magnetic Beads
M047-10 Anti-Myc-tag mAb-Magnetic Agarose
M047-A48 Anti-Myc-tag mAb-Alexa Fluor® 488
M047-A59 Anti-Myc-tag mAb-Alexa Fluor® 594
M047-A64 Anti-Myc-tag mAb-Alexa Fluor® 647
M192-3 Anti-Myc-tag mAb
M192-3S Anti-Myc-tag mAb
M192-7 Anti-Myc-tag mAb-HRP-DirecT
562 Anti-Myc-tag pAb
562-5 Anti-Myc-tag pAb
使用文献
使用文献募集中!本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Ren J et al. Methylation of ribosomal protein S10 by protein-arginine methyltransferase 5 regulates ribosome biogenesis. J Biol Chem. 285,12695-705 (2010)(PMID:20159986)
  2. Sun L et al. Substrate phosphorylation and feedback regulation in JFK-promoted p53 destabilization. J Biol Chem. 286, 4226-35 (2011)(PMID:21127074)
  3. Sato T et al. Identification of 14-3-3 proteins as a target of ATL31 ubiquitin ligase, a regulator of the C/N response in Arabidopsis. Plant J. 68,137-46 (2011)(PMID:21668537)
  4. Fang G et al. Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion. J Cell Sci. 127, 1631-9 (2014)(PMID:24554434)
  5. Chen X et al. Coronavirus membrane-associated papain-like proteases induce autophagy through interacting with Beclin1 to negatively regulate antiviral innate immunity. Protein Cell. 5, 912-27 (2014)(PMID:25311841)
  6. Shin C et al. MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses. Sci Rep. 7, 46097 (2017)(PMID:28378844)
  7. Huang Y et al. RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21. Oncotarget. 8, 83075-83087 (2017)(PMID:29137325)
  8. Yang S et al. USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. Nat Commun. 9, 1285 (2018)(PMID:29599486)
  9. Chen Y et al. Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth. Nat Commun. 9, 2464 (2018)(PMID:29942010)
  10. Yang L et al. Reconstituting Arabidopsis CRY2 Signaling Pathway in Mammalian Cells Reveals Regulation of Transcription by Direct Binding of CRY2 to DNA. Cell Rep. 24, 585-593.e4(2018)(PMID:30021157)
  11. Dansako H et al. High‐level expression of STING restricts susceptibility to HBV by mediating type III IFN induction. FASEB BioAdvances 2018, 1-14 (2018)
  12. Fujibayashi E et al. Isolation of cancer cells with augmented spheroid-forming capability using a novel tool equipped with removable filter. Oncotarget 9, 33931-33946 (2018)(PMID:30338036)
  13. Cui CP et al. Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation. Nat Commun. 9, 4648 (2018)(PMID:30405104)
  14. Yamamoto T et al. DUSP10 constrains innate IL-33-mediated cytokine production in ST2hi memory-type pathogenic Th2 cells. Nat Commun. 9, 4231 (2018)(PMID:30315197)
  15. Ariumi Y et al. DNA repair protein Rad18 restricts LINE-1 mobility. Sci Rep. 8, 15894 (2018)(PMID:30367120)
  16. Gao T et al. PKCζ Phosphorylates SIRT6 to Mediate Fatty Acid β-Oxidation in Colon Cancer Cells. Neoplasia. 21, 61-73 (2019) (PMID:30504065)
  17. Cui CP et al. Author Correction: Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation. Nat Commun. 10, 173 (2019)(PMID:30622263)
  18. Wu R et al. A novel m6A reader Prrc2a controls oligodendroglial specification and myelination. Cell Res. 29, 23-41 (2019) (PMID:30514900)
  19. Fan J et al. CKIP-1 limits foam cell formation and inhibits atherosclerosis by promoting degradation of Oct-1 by REGγ. Nat Commun. 10, 425 (2019)(PMID:30683852)

ChIP

  1. Bermejo R et al. ChIP-on-chip analysis of DNA topoisomerases. Methods Mol Biol. 582, 103-18 (2009)(PMID:19763945)

Immunoprecipitation

  1. Ren J et al. Methylation of ribosomal protein S10 by protein-arginine methyltransferase 5 regulates ribosome biogenesis. J Biol Chem. 285,12695-705 (2010)(PMID:20159986)
  2. Arii J et al. Non-muscle myosin IIA is a functional entry receptor for herpes simplex virus-1. Nature 467, 859-62 (2010)(PMID:20944748)
  3. Sun L et al. Substrate phosphorylation and feedback regulation in JFK-promoted p53 destabilization. J Biol Chem. 286, 4226-35 (2011)(PMID:21127074)
  4. Hirano Y et al. Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain. EMBO J. 30, 2734-47 (2011)(PMID:21642953)
  5. Fang G et al. Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion. J Cell Sci. 127, 1631-9 (2014)(PMID:24554434)
  6. Chen X et al. Coronavirus membrane-associated papain-like proteases induce autophagy through interacting with Beclin1 to negatively regulate antiviral innate immunity. Protein Cell. 5, 912-27 (2014)(PMID:25311841)
  7. Huang Y et al. RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21. Oncotarget. 8, 83075-83087 (2017)(PMID:29137325)
  8. Chen Y et al. Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth. Nat Commun. 9, 2464 (2018)(PMID:29942010)
  9. Yang L et al. Reconstituting Arabidopsis CRY2 Signaling Pathway in Mammalian Cells Reveals Regulation of Transcription by Direct Binding of CRY2 to DNA. Cell Rep. 24, 585-593.e4(2018)(PMID:30021157)
  10. Cui CP et al. Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation. Nat Commun. 9, 4648 (2018)(PMID:30405104)
  11. Yamamoto T et al. DUSP10 constrains innate IL-33-mediated cytokine production in ST2hi memory-type pathogenic Th2 cells. Nat Commun. 9, 4231 (2018)(PMID:30315197)
  12. Ariumi Y et al. DNA repair protein Rad18 restricts LINE-1 mobility. Sci Rep. 8, 15894 (2018)(PMID:30367120)
  13. Cui CP et al. Author Correction: Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation. Nat Commun. 10, 173 (2019)(PMID:30622263)

Immunocytochemistry

  1. Senju Y et al. Essential role of PACSIN2/syndapin-II in caveolae membrane sculpting. J Cell Sci. 124, 2032-40 (2011)(PMID:21610094)
  2. Fang G et al. Centlein mediates an interaction between C-Nap1 and Cep68 to maintain centrosome cohesion. J Cell Sci. 127, 1631-9 (2014)(PMID:24554434)
  3. Shin C et al. MKRN2 is a novel ubiquitin E3 ligase for the p65 subunit of NF-κB and negatively regulates inflammatory responses. Sci Rep. 7, 46097 (2017)(PMID:28378844)

RNP Immunoprecipitation

  1. Tani H et al. Identification of hundreds of novel UPF1 target transcripts by direct determination of whole transcriptome stability. RNA Biol. 9, 1370-9 (2012)(PMID:23064114)
  2. Arimoto-Matsuzaki K  et al. TIA1 oxidation inhibits stress granule assembly and sensitizes cells to stress-induced apoptosis. Nat Commun. 7, 10252 (2016)(PMID:26738979)
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※在庫につきましては2019年07月22日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について:
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について:
*: 論文で報告されております(MBLでは未確認)。詳しくはデータシートをご覧ください。
**: 導入元からの情報です(MBLでは評価中もしくは未確認)。
※保存温度の表記について: RT: 室温
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