トップ > 製品検索結果一覧 > Code No. M044-3 Anti-XIAP (MIHA/ILP-a) mAb

Code No.　M044-3

Anti-XIAP (MIHA/ILP-a) mAb

価格（税別）

¥52,800

在庫

10以上

（2024/04/24 00:05時点）

包装

100 µL (1 mg/mL)

This antibody recognizes C-terminal region of XIAP (352-449 aa) and detects 55 kDa of XIAP on Western Blot using total cell lysate from human or mouse cell line, for example, Jurkat, Raji, NIH/3T3, etc.
データ

  • Western Blotting
クローナリティー Monoclonal クローン 2F1
アイソタイプ
（免疫動物） 		Mouse IgG1
使用法
WB
1 µg/mL  
IH
reported.  （PMID: 15576477 / 18056177 / 11489828）
免疫原(抗原) Recombinant Human full length XIAP
交差性
[Gene ID]

Human[331], Mouse[11798], Rat(w)[63879]
性状 1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度 -20°C 標識 Unlabeled メーカー MBL
別称 X-linked inhibitor of apoptosis, API3, ILP1, XLP2, BIRC4, IAP-3, hIAP3, hIAP-3
背景 Caspase, related to ICE and to CED-3, plays central roles as effectors of apoptosis. Ablation of caspase activity is attained by p35 from baculovirus and CrmA from cowpox, which appear to be suicide inactivators, strongly inhibiting caspase activity. Overexpression of there caspase inhibitors in insect, nematoda and mammalian cells results in resistance to apoptotic stimuli, demonstrating that components of the apoptotic pathway are highly conserved throughout evolution, and leading to the speculation that mammalian functional equivalents of these protease inhibitors may exist. The inhibitor of apoptosis proteins (IAPs) are a family of anti-apoptotic proteins that are conserved across species. Four IAPs have been identified in mammal; NAIP, cIAP1/HIAP2/hMIHB, cIAP2/HIAP1/hMIHC, XIAP/hILP. A prototype of the human IAPs is the XIAP, with a 1.5 kb coding region corresponding to a 55 kDa protein. XIAP can directly inhibit two members of the cell death protease family, caspase-3 and -7.
関連製品 M097-3 Anti-Caspase-3 (Human) mAb
M087-3 Anti-Caspase-3 (Human) mAb
M053-3 Anti-Caspase-7 mAb
M054-3 Anti-Caspase-9 mAb
使用文献
 使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。

Western Blotting

  1. Silke J, et al., Unlike Diablo/smac, Grim promotes global ubiquitination and specific degradation of X chromosome-linked inhibitor of apoptosis (XIAP) and neither cause apoptosis. J Biol Chem. 279, 4313-21. 2004(PMID:14570909)
  2. Tanaka H et al. 3-Phosphoinositide-dependent protein kinase-1-mediated IkappaB kinase beta (IkkB) phosphorylation activates NF-kappaB signaling. J Biol Chem. 280, 40965-73 (2005)(PMID:16207722)
  3. Silke J et al. Determination of cell survival by RING-mediated regulation of inhibitor of apoptosis (IAP) protein abundance. PNAS. 102, 16182-7 (2005)(PMID:16263936)
  4. Marsden VS et al. Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death. Blood. 107, 1872-7 (2006)(PMID:16291596)
  5. Cillessen SA et al. Inhibition of the intrinsic apoptosis pathway downstream of caspase-9 activation causes chemotherapy resistance in diffuse large B-cell lymphoma. Clin Cancer Res. 1, 7012-21 (2007)(PMID:18056177)
  6. Smith MI et al. Skeletal muscle differentiation evokes endogenous XIAP to restrict the apoptotic pathway. PLoS One 4, e5097 (2009)(PMID:19333375)
  7. Verzola D et al. Uric acid promotes apoptosis in human proximal tubule cells by oxidative stress and the activation of NADPH oxidase NOX 4. PLoS One 9, e115210 (2014)(PMID:25514209)
  8. Ishikawa C et al. Mitotic kinase PBK/TOPK as a therapeutic target for adult T‑cell leukemia/lymphoma. Int J Oncol. 53, 801-814 (2018)(PMID:29901068)
  9. Vince JE et al. The Mitochondrial Apoptotic Effectors BAX/BAK Activate Caspase-3 and -7 to Trigger NLRP3 Inflammasome and Caspase-8 Driven IL-1β Activation. Cell Rep. 25, 2339-2353.e4 (2018) (PMID:30485804)
  10. Ishikawa C et al. Evaluation of Artesunate for the Treatment of Adult T-cell leukemia/lymphoma. Eur J Pharmacol. 872, 172953 (2020)(PMID:31996318)
  11. Lalaoui N et al. Targeting triple-negative breast cancers with the Smac-mimetic birinapant. Cell Death Differ. 27, 2768-2780 (2020)(PMID:32341449)

Immunohistochemistry

  1. Ferreira CG et al. Assessment of IAP (inhibitor of apoptosis) proteins as predictors of response to chemotherapy in advanced non-small-cell lung cancer patients. Ann Oncol. 12, 799-805 (2001)(PMID:11484955)
  2. Ferreira CG et al. Expression of X-linked inhibitor of apoptosis as a novel prognostic marker in radically resected non-small cell lung cancer patients. Clin Cancer Res.7, 2468-74 (2001)(PMID:11489828)
  3. Bilim V et al. Role of XIAP in the malignant phenotype of transitional cell cancer (TCC) and therapeutic activity of XIAP antisense oligonucleotides against multidrug-resistant TCC in vitro. Int J Cancer. 103, 29-37 (2003)(PMID:12455050)
  4. Muris JJ et al. Immunohistochemical profiling of caspase signaling pathways predicts clinical response to chemotherapy in primary nodal diffuse large B-cell lymphomas. Blood 105, 2916-23 (2005)(PMID:15576477)
  5. Cillessen SA et al. Inhibition of the intrinsic apoptosis pathway downstream of caspase-9 activation causes chemotherapy resistance in diffuse large B-cell lymphoma. Clin Cancer Res. 1, 7012-21 (2007)(PMID:18056177)
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カテゴリー
分野
アポトーシス
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※在庫につきましては2024年04月24日 00時05分時点における在庫数を表示してあります。
※価格の赤色表示につきましては、キャンペーン価格の表示となっております。
※このサイトからは直接注文はできません。ご注意ください。
※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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