Anti-Np95 (Uhrf1) (Mouse) mAb

価格（税別）

¥52,800

在庫

10以上

（2024/04/20 00:05時点）

包装

100 µL (1 mg/mL)

使用文献

データシート

SDS

データ	Western Blotting Immunocytochemistry
クローナリティー	Monoclonal	クローン	Th-10a
アイソタイプ（免疫動物）	Rat IgG2aκ
使用法	WB 1 µg/mL IP* reported. （PMID: 8634372） FCM* reported. （PMID: 8634372） IC 2 µg/mL IH* reported. （PMID: 8634372）
免疫原(抗原)	Mouse thymic lymphoma
交差性 [Gene ID]	Human(-), Mouse[18140], Rat(-), Hamster(-)
性状	1 mg/mL in PBS/50% glycerol, pH 7.2
保存温度	-20°C	標識	Unlabeled	メーカー	MBL
別称	ICBP90, RNF106, AL022808
背景	DNA methylation inheritance is the process of copying pre-existing methylation patterns onto newly replicated DNA strand after DNA replication. Dnmt1 is the maintenance DNA methyltransferase, which methylates hemimethylated CpGs that appear after DNA repairs and replications. The mouse Np95, also known as Uhrf1, is a nuclear protein, which contains an ubiquitin-like domain, a tandem Tudor domain, a PHD (Plant Homeodomain) finger, an SRA (SET-and RING-associated) domain, and a RING (Really Interesting New Gene) finger domain. Np95 recognizes hemimethylated CpGs via the SRA domain, and recruits Dnmt1 to the sites to transfer the methylation patterns onto the newly synthesized DNA strand. Therefore, the interaction between Np95 and Dnmt1 is necessary for the inheritance of DNA methylation.
関連製品	M202-3 Anti-Np95 (Uhrf1) mAb MK-15-3 Anti-Rb (Human) mAb MH-12-3 Anti-PCNA (Proliferating Cell Nuclear Antigen) mAb M081-3 Rat IgG2a (isotype control) M207-3 Anti-MitoPLD (Pld6) mAb PM044 Anti-PIWIL2 (MILI) (Mouse) pAb RN1005 RIP-Assay Kit for microRNA PM085 Anti-ZBTB24 pAb PM086 Anti-ZBTB24 (Human) pAb D346-3 Anti-5-methylcytidine (m⁵C) mAb
使用文献	使用文献募集中！本製品を使って論文を発表されましたら是非お知らせください。 Western Blotting Fujimori A et al. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. Mamm Genome. 9, 1032-5 (1998)(PMID:9880673) Bonapace IM et al. Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. J Cell Biol. 157, 909-14 (2002)(PMID:12058012) Immunoprecipitation Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995)(PMID:8634372) Flow Cytometry Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995)(PMID:8634372) Immunocytochemistry Uemura T et al. Temporal and spatial localization of novel nuclear protein NP95 in mitotic and meiotic cells. Cell Struct Funct. 25, 149-59 (2000)(PMID:10984098) Bonapace IM et al. Np95 is regulated by E1A during mitotic reactivation of terminally differentiated cells and is essential for S phase entry. J Cell Biol. 157, 909-14 (2002)(PMID:12058012) Sharif J et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA. Nature. 450, 908-12 (2007)(PMID:17994007) Ohno R et al. A replication-dependent passive mechanism modulates DNA demethylation in mouse primordial germ cells. Development 140, 2892-903 (2013)(PMID:23760957) Immunohistochemistry Muto M et al. The characterization of the monoclonal antibody Th-10a, specific for a nuclear protein appearing in the S phase of the cell cycle in normal thymocytes and its unregulated expression in lymphoma cell lines. Cell Prolif. 28, 645-57 (1995)(PMID:8634372) Fujimori A et al. Cloning and mapping of Np95 gene which encodes a novel nuclear protein associated with cell proliferation. Mamm Genome. 9, 1032-5 (1998)(PMID:9880673) Maenohara S et al. Role of UHRF1 in de novo DNA methylation in oocytes and maintenance methylation in preimplantation embryos. PLoS Genet. 13, e1007042 (2017) (PMID:28976982)
製品カテゴリー	分野エピジェネティクスがん

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※使用法の表記について：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation,
FCM: Flow Cytometry NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation,
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
※使用法・交差性の表記について：
*: 論文で報告されております（MBLでは未確認）。詳しくはデータシートをご覧ください。
**: 導入元からの情報です（MBLでは評価中もしくは未確認）。
※保存温度の表記について： RT: 室温
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